Invitrogen

LXR beta Monoclonal Antibody (K8917)

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LXR beta Antibody in Western Blot (WB) — Western blot was performed using anti-LXR beta Monoclonal Antibody (K8917) (Product # 418400) and a 55 kDa band corresponding to LXR beta (NR1H2) was observed across the cell lines tested along with an uncharacterized band at ~ 70 kDa. Modified whole cell extracts (1% SDS) (30 µg lysate) of A549 (Lane 1), PC-3 (Lane 2) and THP-1 (Lane 3) were electrophoresed using Novex® NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (2 µg/mL) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Product Details

418400

Applications	Tested Dilution	Publications
Western Blot (WB)	2 µg/mL	-
ELISA (ELISA)	0.1 µg/mL	-
Immunoprecipitation (IP)	Assay-dependent	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Clone	K8917
Immunogen	Produced in BALB/c mouse ascites after inoculation with hybridoma of mouse myeloma cells (NS-1) and spleen cells derived from a BALB/c mouse immunized with Baculovirus-expressed recombinant human LXR-beta (2-86 aa). View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Ammonium sulfate precipitation
Storage buffer	physiological saline
Contains	0.1% sodium azide
Storage conditions	Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
RRID	AB_2532201

Product Specific Information

This antibody specifically recognizes human LXR beta but does not recognize human LXR alpha. This antibody does not cross react with rat LXR beta. Not yet tested in other species.

Target Information

The liver X receptors, LXRA and LXRB, form a subfamily of the nuclear receptor superfamily and are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. The inducible LXRA is highly expressed in liver, adrenal gland, intestine, adipose tissue, macrophages, lung, and kidney, whereas LXRB is ubiquitously expressed. Ligand-activated LXRs form obligate heterodimers with retinoid X receptors and regulate expression of target genes containing LXR response elements.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: liver X nuclear receptor beta; Liver X receptor beta; LX receptor beta; nuclear orphan receptor LXR-beta; Nuclear receptor NER; Nuclear receptor subfamily 1 group H member 2; nuclear receptor subfamily 1, group H, member 2; Oxysterols receptor LXR-beta; steroid hormone-nuclear receptor NER; Ubiquitously-expressed nuclear receptor

Gene Aliases: LXR-b; LXRB; NER; NER-I; NR1H2; RIP15; UNR

UniProt ID: (Human) E7EWA6

Entrez Gene ID: (Human) 7376

Function(s)