Invitrogen

LYVE1 Monoclonal Antibody (ALY7), eFluor™ 660, eBioscience™

Product Image Gallery

LYVE1 Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Immunohistochemistry of cryosections of mouse intestine at 10 µg/mL of Anti-Mouse Lyve-1 eFluor® 660.

LYVE1 Antibody in Immunocytochemistry (ICC/IF) — Fig. 5 FGFR3 deficiency in LECs aggravates acquired HO formation via upregulating BMPR1a-pSmad1/5 signaling. a - f Representative confocal images of repaired Achilles tendon immunostained with FGFR3 ( a ), BMPR1a ( c ) or pSmad1/5 ( e ) (white), LYVE1 (green), and DAPI (blue) in Col2 tomato (left) and FGFR3 f/f -Col2 tomato mice (right) at 8 weeks after surgery and relative quantitative analysis ( b , d , f ). n = 4 per group. Dashed line boxes indicate tdTomato-labeled LYVE1 + LECs (higher magnification with split channels, right). Scale bars, 100 mum (left); 20 mum (right). g Protein levels of FGFR3, BMPR1a, pSmad1/5, and Smad1/5 in mouse LEC cell line treated with FGFR3 -targeted siRNA (KD) or control siRNA (CON). n = 3 per group. h - m Representative muCT ( h ), SOFG staining ( j ), and OC immunohistochemistry ( l ) images of ectopic bone in the Achilles tendon of FGFR3 Col2 ( n = 4), FGFR3 Col2 ;BMPR1a f/+ ( n = 5), FGFR3 Col2 ;BMPR1a f/f ( n = 5), FGFR3 Prox1 ( n = 4), FGFR3 Prox1 ;BMPR1a f/+ ( n = 10), and FGFR3 Prox1 ;BMPR1a f/f mice ( n = 6) at 8 weeks after surgery and relative quantitative analysis ( i , k , m ). Scale bars, 1 mm for muCT; 200 mum for SOFG and OC IHC. n - q Representative confocal images of Achilles tendon immunostained with LYVE1 (green) and DAPI (blue) ( n ) in FGFR3 Col2 ( n = 4), FGFR3 Col2 ;BMPR1a f/+ ( n = 5), FGFR3 Col2 ;BMPR1a f/f ( n = 5), FGFR3 Prox1 ( n = 4), FGFR3 Prox1 ;BMPR1a f/+ ( n = 5), and FGFR3 Prox1 ;BMPR1a f/f mice ( n = 6) and

LYVE1 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC) — Figure 7. Long-term effect of topical dexamethasone treatment during the first 8 d of HSV-1 infection on corneal innervation and vasculature. Mouse corneas were infected with 10 3 PFU HSV-1 or left U) as controls. Starting at 2 h p.i., mice were topically treated with DEX or VEH onto their corneas for 8 d p.i. before tissue collection at 30 d p.i. (A) Representative confocal images show corneal nerves (green: beta-III tubulin staining) and blood and lymphatic vessels (red: CD31 staining; blue: LYVE-1 staining, top discontinuous white lines depict the limbal margins) at 30 d p.i. (top: merge of beta-III tubulin/CD3/LYVE-1 costaining, bottom: grayscale display of beta-III tubulin staining only). White arrows depict intact fine bundles of subbasal nerves in the UI group that are preserved in the infected group treated with DEX. (B) Analysis of corneal innervation (left) and vascularization (center and right) expressed as the percentage threshold area positive for beta-III tubulin signal per field of view means +- sem , percentage threshold area positive for CD31 signal per field of view means +- sem , and percentage threshold area positive for LYVE-1 signal per field of view means +- sem, respectively ( n = 6 for UI and n = 16-17 for infected groups for beta-III tubulin, n = 11 for UI and n = 16-23 for uninfected groups for CD31, and n = 9 for UI and n = 11-16 per infected groups for LYVE-1 from 3-4 independent experiments). (C) Bars show Cochet-Bonnet

LYVE1 Antibody in Immunohistochemistry (IHC) — Figure 7 Z-projection through a stack of confocal micrographs of E14.5 fetal liver section (A) Representative image shows CD31 + pan-endothelium (red), Lyve-1 + sinusoidal endothelium (green) and Hoechst counter-stained nuclei (blue). (B) Representative image shows CD31 + pan-endothelium (red), 3 + cells (white) and Sca-1 + cells (green); Yellow arrows mark 3 - Sca-1 + HSCs. Scale bar: 20 mum.

LYVE1 Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow) — Fig. 1 Brain tumors induce dorsal meningeal lymphangiogenesis. a, b Left, Representative meningeal LYVE-1 staining 1 week after subdural injection ( a ) and 2 weeks after striatal injection ( b ) of GL261 or B16 cells into WT mice (SSS superior sagittal sinus, TS transverse sinus). Right, Quantification of the diameter ( n = 12) and percentage area ( n = 10) of LYVE-1 + MLVs around the TS. Scale bars, 500 um in wide-fields; 100 um in insets. c Schematic diagram of tamoxifen administration and tissue analysis schedule in Prox1-Cre ERT2+ ; R26-tdTomato + mice. d Representative FACS plots and gating scheme of CD31 + LYVE-1 + tdTomato + MLECs isolated from normal Prox1-Cre ERT2 - ; R26-tdTomato + and Prox1-Cre ERT2+ ; R26-tdTomato + mice 3 weeks after tamoxifen induction. e Images of Prox1, LYVE-1 staining and tdTomato signals in the TS of meninges from Prox1-Cre ERT2 - ; R26-tdTomato + and Prox1-Cre ERT2+ ; R26-tdTomato + mice 3 weeks after tamoxifen induction. Scale bars, 20 um. f LYVE-1 staining of MLVs around the TS in Prox1-Cre ERT2+ ; R26-tdTomato + mice 2 weeks after subdural injection of GL261 or B16 cells. Scale bars, 100 um in wide-fields; 50 um in insets. g Co-localization analysis of tdTomato and LYVE-1 in the insets shown in f . Data are presented as means +- SEM; each symbol represents an individual mouse. ** P < 0.01, *** P < 0.001; two-way ANOVA ( a , b ). Data are from at least three independent experiments ( a - g ).

LYVE1 Antibody in Flow Cytometry (Flow) — Fig. 7 CX3CR1 + pvM numbers increase after induced ischemic stroke. a Maximal intensity projections (sagittal view) (2660 um) of lightsheet acquisitions on cleared brains, stained for Lyve1 (red), control and after stroke (P14 = 14 days after induction of ischemic stroke) (2 individual experiments, n = 7 animals) (Supplementary Movies 5 and 6 ). b Quantification of Lyve1 in the area containing the whole stroke lesion within the dorsal cortex situated just above the hippocampus, imaged by lightsheet microscopy. * represents P -value <=0.05 (Ordinary one-way Anova test). c Representative flow cytometry plots from Cx3cr1 GFP brain parenchyma 14 days after induced stroke (P14), pre-gated on living, single and showing Lyve1 + F4/80 + cells and CX3CR1 + /CX3CR1 - gating for these cells Lyve1 + F4/80 + and subsequently in blue the CD45 expression of the Lyve1 + F4/80 + CX3CR1 - , in red the CD45 expression of the Lyve1 + F4/80 + CX3CR1 + and FMO in gray. Microglia cells were excluded since they are Lyve1 - . Average percentages for the different subpopulations are shown in the plots ( n = 4). d Graphs representing the Lyve1 + F4/80 + cell % and Lyve1 + F4/80 + CX3CR1 + cell % showing an increase of these populations at P3, P8, and P14 after stroke by flow cytometry and Lyve1 + F4/80 + CX3CR1 - cell % showing a decrease of this population at P3, P8, and P14 after stroke. Data are presented as mean value +- SD. Asterisk (*) represents P -value <=0.05, ** P -value <=0.01, *** P -value

Product Details

50-0443-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 16 publications 16 publications

Immunohistochemistry (Frozen) (IHC (F))

1-10 µg/mL

Immunocytochemistry (ICC/IF)

1-10 µg/mL

View 4 publications 4 publications

Flow Cytometry (Flow)

Assay-Dependent

View 14 publications 14 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), eFluor™ 660, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

ALY7

Conjugate

eFluor™ 660

Excitation/Emission Max

651/668 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

RRID

AB_10597449

Product Specific Information

Description: The monoclonal antibody ALY7 recognizes mouse LYVE-1, a transmembrane glycoprotein with similarity to CD44. The extracellular domain contains a conserved hyaluronan binding domain also found in CD44. Expression is found on lymphatic and liver endothelial cells and some populations of macrophages. The lymphatic system is responsible for transporting proteins and cells (especially dendritic cells) to tissues throughout the body, thereby acting as immune surveyors. LYVE-1 is one characteristic protein, along with podoplanin, PROX-1, Tie-2 and VEGFR-3, that is expressed on lymphatic endothelial cells (LECS). The ligand for LYVE-1 is hyaluronan, a large mucopolysaccharide. Although LYVE-1 can bind hyaluronan in vitro, the site for ligand binding in vivo is masked by sialyated O-linked glycan chains. It is postulated that binding to ligand requires modification/unmasking to expose the binding site. The development and remodeling of the endothelium after injury is an area of extensive study. When transplanted, hematopoietic stem cells (HSCs) can give rise to LECs that integrate into the endothelium in normal and metastatic tissue.

Applications Reported: This ALY7 antibody has been reported for use in flow cytometric analysis and immunohistology staining of frozen tissue sections.

Applications Tested: This ALY7 antibody has been tested by flow cytometric analysis of transfected cells or IHC on mouse tissue. This can be used at 1-10 µg/mL for IHC. It is recommended to titer the antibody in the application of interest.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

LYVE1 has been identified as a major receptor for HA (extracellular matrix glycosaminoglycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE1 predicts a 322-residue type I integral membrane polypeptide 41% similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module, the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE1 molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE1 molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves. LYVE1 is a type I integral membrane glycoprotein. LYVE-1 is expressed primarily on lymphatic vessel endothelium and is likely to be involved in regulating the traffic of leucocytes and tumor cells to lymph nodes. The lymphatic vasculature forms a second circulatory system that drains extracellular fluid from the tissues and provides an exclusive environment in which immune cells can encounter and respond to foreign antigen. A number of molecules have been identified as markers for lymphatic endothelium which include LYVE1, PALE, VEGFR3, and podoplanin. Diseases associated with LYVE1 dysfunction includes Complete Androgen Insensitivity Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Immuno-localization of definitive hematopoietic stem cells in the vascular niche of mouse fetal liver.

STAR protocols

Biswas A,Singh SK,Kartha GM,Khurana S

Published figure using LYVE1 monoclonal antibody (Product # 50-0443-82) in Immunohistochemistry

Fri Dec 16 00:00:00 EST 2022

Deciphering the heterogeneity of the Lyve1⁺ perivascular macrophages in the mouse brain.

Nature communications

Siret C,van Lessen M,Bavais J,Jeong HW,Reddy Samawar SK,Kapupara K,Wang S,Simic M,de Fabritus L,Tchoghandjian A,Fallet M,Huang H,Sarrazin S,Sieweke MH,Stumm R,Sorokin L,Adams RH,Schulte-Merker S,Kiefer F,van de Pavert SA

Published figure using LYVE1 monoclonal antibody (Product # 50-0443-82) in Flow Cytometry

Wed Nov 30 00:00:00 EST 2022

Deciphering the heterogeneity of the Lyve1⁺ perivascular macrophages in the mouse brain.

Nature communications

Published figure using LYVE1 monoclonal antibody (Product # 50-0443-82) in Flow Cytometry

Wed Nov 30 00:00:00 EST 2022

Hypoxia shapes the immune landscape in lung injury and promotes the persistence of inflammation.

Nature immunology

Mirchandani AS,Jenkins SJ,Bain CC,Sanchez-Garcia MA,Lawson H,Coelho P,Murphy F,Griffith DM,Zhang A,Morrison T,Ly T,Arienti S,Sadiku P,Watts ER,Dickinson RS,Reyes L,Cooper G,Clark S,Lewis D,Kelly V,Spanos C,Musgrave KM,Delaney L,Harper I,Scott J,Parkinson NJ,Rostron AJ,Baillie JK,Clohisey S,Pridans C,Campana L,Lewis PS,Simpson AJ,Dockrell DH,Schwarze J,Hirani N,Ratcliffe PJ,Pugh CW,Kranc K,Forbes SJ,Whyte MKB,Walmsley SR

50-0443-82 was used in Immunohistochemistry-immunofluorescence to investigate how hypoxia affects immune landscape in ARDS patients.

Wed Jun 01 00:00:00 EDT 2022

Extracellular ATP Limits Homeostatic T Cell Migration Within Lymph Nodes.

Frontiers in immunology

Kobayashi D,Sugiura Y,Umemoto E,Takeda A,Ueta H,Hayasaka H,Matsuzaki S,Katakai T,Suematsu M,Hamachi I,Yegutkin GG,Salmi M,Jalkanen S,Miyasaka M

50-0443-82 was used in Immunohistochemistry-immunofluorescence to indicate that paracortical eATP is produced by naive T cells in response to constitutively expressed chemokines, and that eATP negatively regulates CCR7-mediated lymphocyte migration within LNs via a specific subtype of ATP receptor, demonstrating its fine-tuning role in homeostatic cell migration within LNs.

Mon Feb 14 00:00:00 EST 2022

Extracellular ATP Limits Homeostatic T Cell Migration Within Lymph Nodes.

Frontiers in immunology

Kobayashi D,Sugiura Y,Umemoto E,Takeda A,Ueta H,Hayasaka H,Matsuzaki S,Katakai T,Suematsu M,Hamachi I,Yegutkin GG,Salmi M,Jalkanen S,Miyasaka M

Mon Feb 14 00:00:00 EST 2022

Nasal administration of mitochondria reverses chemotherapy-induced cognitive deficits.

Theranostics

Alexander JF,Seua AV,Arroyo LD,Ray PR,Wangzhou A,Heiβ-Lückemann L,Schedlowski M,Price TJ,Kavelaars A,Heijnen CJ

Published figure using LYVE1 monoclonal antibody (Product # 50-0443-82) in Immunocytochemistry

Tue Jul 27 00:00:00 EDT 2021

Targeting local lymphatics to ameliorate heterotopic ossification via FGFR3-BMPR1a pathway.

Nature communications

Zhang D,Huang J,Sun X,Chen H,Huang S,Yang J,Du X,Tan Q,Luo F,Zhang R,Zhou S,Jiang W,Ni Z,Wang Z,Jin M,Xu M,Li F,Chen L,Liu M,Su N,Luo X,Yin L,Zhu Y,Feng JQ,Chen D,Qi H,Chen L,Xie Y

Published figure using LYVE1 monoclonal antibody (Product # 50-0443-82) in Immunocytochemistry

Mon Jul 19 00:00:00 EDT 2021

Efficient Tissue Clearing and Multi-Organ Volumetric Imaging Enable Quantitative Visualization of Sparse Immune Cell Populations During Inflammation.

Frontiers in immunology

Hofmann J,Gadjalova I,Mishra R,Ruland J,Keppler SJ

Published figure using LYVE1 monoclonal antibody (Product # 50-0443-82) in Immunocytochemistry

Wed Jun 16 00:00:00 EDT 2021

Interleukin-11-expressing fibroblasts have a unique gene signature correlated with poor prognosis of colorectal cancer.

Nature communications

Nishina T,Deguchi Y,Ohshima D,Takeda W,Ohtsuka M,Shichino S,Ueha S,Yamazaki S,Kawauchi M,Nakamura E,Nishiyama C,Kojima Y,Adachi-Akahane S,Hasegawa M,Nakayama M,Oshima M,Yagita H,Shibuya K,Mikami T,Inohara N,Matsushima K,Tada N,Nakano H

Published figure using LYVE1 monoclonal antibody (Product # 50-0443-82) in Flow Cytometry

Fri Apr 16 00:00:00 EDT 2021

Integrins mediate placental extracellular vesicle trafficking to lung and liver in vivo.

Scientific reports

Nguyen SL,Ahn SH,Greenberg JW,Collaer BW,Agnew DW,Arora R,Petroff MG

Published figure using LYVE1 monoclonal antibody (Product # 50-0443-82) in Immunohistochemistry

Thu Feb 18 00:00:00 EST 2021

Anatomic evidence shows that lymphatic drainage exists in the pituitary to loop the cerebral lymphatic circulation.

Medical hypotheses

Elham E,Wumaier R,Wang C,Luo X,Chen T,Zhong N

Published figure using LYVE1 monoclonal antibody (Product # 50-0443-82) in Immunohistochemistry

Thu Oct 01 00:00:00 EDT 2020

Complex Non-sinus-associated Pachymeningeal Lymphatic Structures: Interrelationship With Blood Microvasculature.

Frontiers in physiology

Glinskii OV,Huxley VH,Xie L,Bunyak F,Palaniappan K,Glinsky VV

Published figure using LYVE1 monoclonal antibody (Product # 50-0443-82) in Immunofluorescence

Tue Sep 29 00:00:00 EDT 2020

Meningeal lymphatic dysfunction exacerbates traumatic brain injury pathogenesis.

Nature communications

Bolte AC,Dutta AB,Hurt ME,Smirnov I,Kovacs MA,McKee CA,Ennerfelt HE,Shapiro D,Nguyen BH,Frost EL,Lammert CR,Kipnis J,Lukens JR

Published figure using LYVE1 monoclonal antibody (Product # 50-0443-82) in Immunohistochemistry

Thu Sep 10 00:00:00 EDT 2020

FK506 induces lung lymphatic endothelial cell senescence and downregulates LYVE-1 expression, with associated decreased hyaluronan uptake.

Molecular medicine (Cambridge, Mass.)

Shrestha S,Cho W,Stump B,Imani J,Lamattina AM,Louis PH,Pazzanese J,Rosas IO,Visner G,Perrella MA,El-Chemaly S

Published figure using LYVE1 monoclonal antibody (Product # 50-0443-82) in Flow Cytometry

Fri Jul 31 00:00:00 EDT 2020

Alveolar regeneration through a Krt8+ transitional stem cell state that persists in human lung fibrosis.

Nature communications

Strunz M,Simon LM,Ansari M,Kathiriya JJ,Angelidis I,Mayr CH,Tsidiridis G,Lange M,Mattner LF,Yee M,Ogar P,Sengupta A,Kukhtevich I,Schneider R,Zhao Z,Voss C,Stoeger T,Neumann JHL,Hilgendorff A,Behr J,O'Reilly M,Lehmann M,Burgstaller G,Königshoff M,Chapman HA,Theis FJ,Schiller HB

50-0443 was used in Flow cytometry/Cell sorting to propose that in lung fibrosis, perturbed molecular checkpoints on the way to terminal differentiation can cause aberrant persistence of regenerative intermediate stem cell states.

Thu Jul 16 00:00:00 EDT 2020

Structural cells are key regulators of organ-specific immune responses.

Nature

Krausgruber T,Fortelny N,Fife-Gernedl V,Senekowitsch M,Schuster LC,Lercher A,Nemc A,Schmidl C,Rendeiro AF,Bergthaler A,Bock C

50-0443 was used in Flow cytometry/Cell sorting to investigate the regulation of and characterise the immune genes in epithelia and endothelia structural cells and fibroblasts using mouse specimens.

Wed Jul 01 00:00:00 EDT 2020

Meningeal lymphatics clear erythrocytes that arise from subarachnoid hemorrhage.

Nature communications

Chen J,Wang L,Xu H,Xing L,Zhuang Z,Zheng Y,Li X,Wang C,Chen S,Guo Z,Liang Q,Wang Y

50-0443 was used in Immunohistochemistry-immunofluorescence to demonstrate that the meningeal lymphatics drain extravasated erythrocytes from CSF into CLNs after SAH, while suggesting that modulating this draining may offer therapeutic approaches to alleviate SAH severity.

Mon Jun 22 00:00:00 EDT 2020

Meningeal lymphatics clear erythrocytes that arise from subarachnoid hemorrhage.

Nature communications

Chen J,Wang L,Xu H,Xing L,Zhuang Z,Zheng Y,Li X,Wang C,Chen S,Guo Z,Liang Q,Wang Y

Mon Jun 22 00:00:00 EDT 2020

Tissue-resident macrophages in omentum promote metastatic spread of ovarian cancer.

The Journal of experimental medicine

Etzerodt A,Moulin M,Doktor TK,Delfini M,Mossadegh-Keller N,Bajenoff M,Sieweke MH,Moestrup SK,Auphan-Anezin N,Lawrence T

Published figure using LYVE1 monoclonal antibody (Product # 50-0443-82) in Flow Cytometry

Mon Apr 06 00:00:00 EDT 2020

Meningeal lymphatic vessels regulate brain tumor drainage and immunity.

Cell research

Hu X,Deng Q,Ma L,Li Q,Chen Y,Liao Y,Zhou F,Zhang C,Shao L,Feng J,He T,Ning W,Kong Y,Huo Y,He A,Liu B,Zhang J,Adams R,He Y,Tang F,Bian X,Luo J

Published figure using LYVE1 monoclonal antibody (Product # 50-0443-82) in Flow Cytometry

Sun Mar 01 00:00:00 EST 2020

Meningeal lymphatic vessels regulate brain tumor drainage and immunity.

Cell research

Hu X,Deng Q,Ma L,Li Q,Chen Y,Liao Y,Zhou F,Zhang C,Shao L,Feng J,He T,Ning W,Kong Y,Huo Y,He A,Liu B,Zhang J,Adams R,He Y,Tang F,Bian X,Luo J

Published figure using LYVE1 monoclonal antibody (Product # 50-0443-82) in Flow Cytometry

Sun Mar 01 00:00:00 EST 2020

Stellate Cells, Hepatocytes, and Endothelial Cells Imprint the Kupffer Cell Identity on Monocytes Colonizing the Liver Macrophage Niche.

Immunity

Bonnardel J,T'Jonck W,Gaublomme D,Browaeys R,Scott CL,Martens L,Vanneste B,De Prijck S,Nedospasov SA,Kremer A,Van Hamme E,Borghgraef P,Toussaint W,De Bleser P,Mannaerts I,Beschin A,van Grunsven LA,Lambrecht BN,Taghon T,Lippens S,Elewaut D,Saeys Y,Guilliams M

50-0443-82 was used in Flow Cytometry to show that the Kupffer cell niche is composed of stellate cells, hepatocytes, and endothelial cells that together imprint the liver-specific macrophage identity.

Tue Oct 15 00:00:00 EDT 2019

Neuroinflammation-induced lymphangiogenesis near the cribriform plate contributes to drainage of CNS-derived antigens and immune cells.

Nature communications

Hsu M,Rayasam A,Kijak JA,Choi YH,Harding JS,Marcus SA,Karpus WJ,Sandor M,Fabry Z

50-0443 was used in Immunohistochemistry to show that increased lymphangiogenesis near the cribriform plate can contribute to the management of neuroinflammation-induced fluid accumulation and immune surveillance.

Wed Jan 16 00:00:00 EST 2019

CNS lymphatic drainage and neuroinflammation are regulated by meningeal lymphatic vasculature.

Nature neuroscience

Louveau A,Herz J,Alme MN,Salvador AF,Dong MQ,Viar KE,Herod SG,Knopp J,Setliff JC,Lupi AL,Da Mesquita S,Frost EL,Gaultier A,Harris TH,Cao R,Hu S,Lukens JR,Smirnov I,Overall CC,Oliver G,Kipnis J

50-0443 was used in Immunohistochemistry to demonstrate that meningeal lymphatics fulfill two critical criteria: they assist in the drainage of cerebrospinal fluid components and enable immune cells to enter draining lymph nodes in a CCR7-dependent manner.

Mon Oct 01 00:00:00 EDT 2018

Hyaluronan Receptor LYVE-1-Expressing Macrophages Maintain Arterial Tone through Hyaluronan-Mediated Regulation of Smooth Muscle Cell Collagen.

Immunity

Lim HY,Lim SY,Tan CK,Thiam CH,Goh CC,Carbajo D,Chew SHS,See P,Chakarov S,Wang XN,Lim LH,Johnson LA,Lum J,Fong CY,Bongso A,Biswas A,Goh C,Evrard M,Yeo KP,Basu R,Wang JK,Tan Y,Jain R,Tikoo S,Choong C,Weninger W,Poidinger M,Stanley RE,Collin M,Tan NS,Ng LG,Jackson DG,Ginhoux F,Angeli V

50-0443-82 was used in Quantitative reverse transcription PCR to unveil a hitherto unknown homeostatic contribution of arterial LYVE-1+ macrophages through the control of collagen production by SMCs and has identified a function of LYVE-1 in leukocytes.

Tue Aug 21 00:00:00 EDT 2018

ADAM10-Mediated ICOS Ligand Shedding on B Cells Is Necessary for Proper T Cell ICOS Regulation and T Follicular Helper Responses.

Journal of immunology (Baltimore, Md. : 1950)

Lownik JC,Luker AJ,Damle SR,Cooley LF,El Sayed R,Hutloff A,Pitzalis C,Martin RK,El Shikh MEM,Conrad DH

50-0443 was used in Immunohistochemistry to demonstrate that a disintegrin and metalloproteinase (ADAM)10 is the primary physiological sheddase of ICOS ligand in mice and humans.

Sun Oct 01 00:00:00 EDT 2017

FGF-dependent metabolic control of vascular development.

Nature

Yu P,Wilhelm K,Dubrac A,Tung JK,Alves TC,Fang JS,Xie Y,Zhu J,Chen Z,De Smet F,Zhang J,Jin SW,Sun L,Sun H,Kibbey RG,Hirschi KK,Hay N,Carmeliet P,Chittenden TW,Eichmann A,Potente M,Simons M

50-0443 was used in Flow cytometry/Cell sorting to identify FGF receptor signalling as a critical regulator of vascular development.

Thu May 11 00:00:00 EDT 2017

Long-term consequences of topical dexamethasone treatment during acute corneal HSV-1 infection on the immune system.

Journal of leukocyte biology

Chucair-Elliott AJ,Carr MM,Carr DJJ

Published figure using LYVE1 monoclonal antibody (Product # 50-0443-82) in Immunofluorescence

Mon May 01 00:00:00 EDT 2017

Long-term consequences of topical dexamethasone treatment during acute corneal HSV-1 infection on the immune system.

Journal of leukocyte biology

Chucair-Elliott AJ,Carr MM,Carr DJJ

Published figure using LYVE1 monoclonal antibody (Product # 50-0443-82) in Immunofluorescence

Mon May 01 00:00:00 EDT 2017

Lymphatic Endothelial Cells Produce M-CSF, Causing Massive Bone Loss in Mice.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Wang W,Wang H,Zhou X,Li X,Sun W,Dellinger M,Boyce BF,Xing L

Published figure using LYVE1 monoclonal antibody (Product # 50-0443-82) in Flow Cytometry

Mon May 01 00:00:00 EDT 2017

Lymphatic Endothelial Cells Produce M-CSF, Causing Massive Bone Loss in Mice.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Wang W,Wang H,Zhou X,Li X,Sun W,Dellinger M,Boyce BF,Xing L

Published figure using LYVE1 monoclonal antibody (Product # 50-0443-82) in Flow Cytometry

Mon May 01 00:00:00 EDT 2017

Defective lymphatic valve development and chylothorax in mice with a lymphatic-specific deletion of Connexin43.

Developmental biology

Munger SJ,Davis MJ,Simon AM

Published figure using LYVE1 monoclonal antibody (Product # 50-0443-82) in Immunohistochemistry

Sun Jan 15 00:00:00 EST 2017

Diphtheria toxin-mediated ablation of lymphatic endothelial cells results in progressive lymphedema.

JCI insight

Gardenier JC,Hespe GE,Kataru RP,Savetsky IL,Torrisi JS,Nores GDG,Dayan JJ,Chang D,Zampell J,Martínez-Corral I,Ortega S,Mehrara BJ

Published figure using LYVE1 monoclonal antibody (Product # 50-0443-82) in Flow Cytometry

Thu Sep 22 00:00:00 EDT 2016

Segregated Foxc2, NFATc1 and Connexin expression at normal developing venous valves, and Connexin-specific differences in the valve phenotypes of Cx37, Cx43, and Cx47 knockout mice.

Developmental biology

Munger SJ,Geng X,Srinivasan RS,Witte MH,Paul DL,Simon AM

Published figure using LYVE1 monoclonal antibody (Product # 50-0443-82) in Immunofluorescence

Fri Apr 15 00:00:00 EDT 2016

Lymphatic endothelial cells efferent to inflamed joints produce iNOS and inhibit lymphatic vessel contraction and drainage in TNF-induced arthritis in mice.

Arthritis research & therapy

Liang Q,Ju Y,Chen Y,Wang W,Li J,Zhang L,Xu H,Wood RW,Schwarz EM,Boyce BF,Wang Y,Xing L

Published figure using LYVE1 monoclonal antibody (Product # 50-0443-82) in Flow Cytometry

Sat Mar 12 00:00:00 EST 2016

Nonvenous origin of dermal lymphatic vasculature.

Circulation research

Martinez-Corral I,Ulvmar MH,Stanczuk L,Tatin F,Kizhatil K,John SW,Alitalo K,Ortega S,Makinen T

50-0443 was used in Flow cytometry/Cell sorting to investigate the origin of murine lymphatic vasculature, showing a significant contribution from nonvenous cells.

Fri May 08 00:00:00 EDT 2015

Identification of targets of Prox1 during in vitro vascular differentiation from embryonic stem cells: functional roles of HoxD8 in lymphangiogenesis.

Journal of cell science

Harada K,Yamazaki T,Iwata C,Yoshimatsu Y,Sase H,Mishima K,Morishita Y,Hirashima M,Oike Y,Suda T,Miura N,Watabe T,Miyazono K

Sun Nov 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: cell surface retention sequence binding protein-1; Cell surface retention sequence-binding protein 1; CRSBP-1; extra cellular link domain-containing 1; Extracellular link domain-containing protein 1; lymphatic vessel endothelial HA receptor-1; lymphatic vessel endothelial HA recptor-1; Lymphatic vessel endothelial hyaluronic acid receptor 1; LYVE-1; sLyve 1; sLyve1; soluble LYVE 1; soluble LYVE1

Gene Aliases: 1200012G08Rik; Crsbp-1; Crsbp1; Lyve-1; Lyve1; Xlkd1

UniProt ID: (Mouse) Q8BHC0

Entrez Gene ID: (Mouse) 114332

Molecular Function: transmembrane signal receptor

Function(s)

glycosaminoglycan catabolic process transport cell adhesion signal transduction

Process(es)

transmembrane signaling receptor activity hyaluronic acid binding

LYVE1 Monoclonal Antibody (ALY7), eFluor™ 660, eBioscience™

Product Details

50-0443-82

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

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Miscellaneous PubMed (Misc)

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