|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 133 and 332 of Human Lactate Dehydrogenase|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
PA5-27406 targets Lactate Dehydrogenase in IF, IHC (P), and WB applications and shows reactivity with Human samples.
The PA5-27406 immunogen is recombinant fragment corresponding to a region within amino acids 133 and 332 of Human Lactate Dehydrogenase.
The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Breast cancer stem cells rely on fermentative glycolysis and are sensitive to 2-deoxyglucose treatment.
PA5-27406 was used in western blot to identify the metabolic phenotype associated with breast cancer stem cells
|Ciavardelli D,Rossi C,Barcaroli D,Volpe S,Consalvo A,Zucchelli M,De Cola A,Scavo E,Carollo R,D'Agostino D,Forlì F,D'Aguanno S,Todaro M,Stassi G,Di Ilio C,De Laurenzi V,Urbani A||Cell death and disease (5:null)||2014|
p63 isoforms regulate metabolism of cancer stem cells.
PA5-27406 was used in western blot to study the modulation of colon cancer stem cell metabolism by p63
|D'Aguanno S,Barcaroli D,Rossi C,Zucchelli M,Ciavardelli D,Cortese C,De Cola A,Volpe S,D'Agostino D,Todaro M,Stassi G,Di Ilio C,Urbani A,De Laurenzi V||Journal of proteome research (13:2120)||2014|