Proteintech

Lamin A/C Polyclonal Antibody

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

Lamin A/C Antibody in Immunocytochemistry (ICC/IF) — This antibody specifically recognizes the heavy chain of Rabbit IgG. It can't detect the The heavy chain of other species. This antibody can be used as the secondary antibody which is specific to the heavy chain of Rabbit IgG.

Lamin A/C Antibody in Western Blot (WB) — Mouse heart tissue were subjected to SDS PAGE followed by western blot with 10298-1-AP (lamin-A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Lamin A/C Antibody in Flow Cytometry (Flow) — 1X10^6 HEK-293T cells were stained with 0.2ug Lamin A/C antibody (10298-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

Lamin A/C Antibody in Immunoprecipitation (IP) — IP result of anti-lamin-A (IP:10298-1-AP, 3ug; Detection:10298-1-AP 1:1000) with A375 cells lysate 800ug.

Product Details

10298-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:6,000	View 33 publications 33 publications
Immunocytochemistry (ICC/IF)	1:50-1:500	-
Flow Cytometry (Flow)	0.2 µg/1x10^6 cells	-
Immunoprecipitation (IP)	0.5-4.0 µg	-
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Avian, Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Lamin A/C Fusion Protein Ag0408 (26-198 aa encoded by BC003162) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.45 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.1% sodium azide
Storage conditions	-20°C

Product Specific Information

This antibody can recognize 4 isoforms of Lamin A/C.

Immunogen sequence: ITRLQEKED LQELNDRLAV YIDRVRSLET ENAGLRLRIT ESEEVVSREV SGIKAAYEAE LGDARKTLDS VAKERARLQL ELSKVREEFK ELKARNTKKE GDLIAAQARL KDLEALLNSK EAALSTALSE KRTLEGELHD LRGQVAKLEA ALGEAKKQLQ DEMLRRVDAE NRLQ (26-198 aa encoded by BC003162)

Target Information

Lamins are a class of intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope. These proteins are found in many different cell types in three different forms (A, B, and C). Lamins A and C are alternatively spliced versions of the LMNA gene. The LMNA gene has been linked to many disorders of the muscular system, nervous system, and the fat distributions systems including: Emery-Dreifuss muscular dystrophy, Dunnigan-type familial partial lipodystrophy (FPLD), limb-girdle muscular dystrophy (LGMD1B), dilated cardiomyopathy (CMD1A), axonal neuropathy (Charcot-Marie-Tooth disease; CMT2B1), and mandibuloacral dysplasia (MAD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

DExD/H-Box Helicase 36 Signaling via Myeloid Differentiation Primary Response Gene 88 Contributes to NF-κB Activation to Type 2 Porcine Reproductive and Respiratory Syndrome Virus Infection.

Frontiers in immunology

Jing H,Zhou Y,Fang L,Ding Z,Wang D,Ke W,Chen H,Xiao S

Wed Nov 20 00:00:00 EST 2019

Lamin A mutation impairs interaction with nucleoporin NUP155 and disrupts nucleocytoplasmic transport in atrial fibrillation.

Human mutation

Han M,Zhao M,Cheng C,Huang Y,Han S,Li W,Tu X,Luo X,Yu X,Liu Y,Chen Q,Ren X,Wang QK,Ke T

Fri Mar 01 00:00:00 EST 2019

HSPA12A Is a Novel Player in Nonalcoholic Steatohepatitis via Promoting Nuclear PKM2-Mediated M1 Macrophage Polarization.

Diabetes

Kong Q,Li N,Cheng H,Zhang X,Cao X,Qi T,Dai L,Zhang Z,Chen X,Li C,Li Y,Xue B,Fang L,Liu L,Ding Z

Fri Feb 01 00:00:00 EST 2019

Baicalein protects human vitiligo melanocytes from oxidative stress through activation of NF-E2-related factor2 (Nrf2) signaling pathway.

Free radical biology & medicine

Ma J,Li S,Zhu L,Guo S,Yi X,Cui T,He Y,Chang Y,Liu B,Li C,Jian Z

Sat Dec 01 00:00:00 EST 2018

Paracrine HGF/c-MET enhances the stem cell-like potential and glycolysis of pancreatic cancer cells via activation of YAP/HIF-1α.

Experimental cell research

Yan B,Jiang Z,Cheng L,Chen K,Zhou C,Sun L,Qian W,Li J,Cao J,Xu Q,Ma Q,Lei J

Mon Oct 01 00:00:00 EDT 2018

Matrix stiffness mediates stemness characteristics via activating the Yes-associated protein in colorectal cancer cells.

Journal of cellular biochemistry

Tan F,Huang Y,Pei Q,Liu H,Pei H,Zhu H

Fri Sep 14 00:00:00 EDT 2018

Calycosin alleviates allergic contact dermatitis by repairing epithelial tight junctions via down-regulating HIF-1α.

Journal of cellular and molecular medicine

Jia Z,Wang X,Wang X,Wei P,Li L,Wu P,Hong M

Sat Sep 01 00:00:00 EDT 2018

Role of c-Abl-GSK3β Signaling in MPP+-Induced Autophagy-Lysosomal Dysfunction.

Toxicological sciences : an official journal of the Society of Toxicology

Ren Y,Chen J,Wu X,Gui C,Mao K,Zou F,Li W

Sat Sep 01 00:00:00 EDT 2018

SKP2 Promotes Hepatocellular Carcinoma Progression Through Nuclear AMPK-SKP2-CARM1 Signaling Transcriptionally Regulating Nutrient-Deprived Autophagy Induction.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Wei X,Li X,Yan W,Zhang X,Sun Y,Zhang F

Fri Aug 17 00:00:00 EDT 2018

Hypoxia-Induced Mitogenic Factor Promotes Cardiac Hypertrophy via Calcium-Dependent and Hypoxia-Inducible Factor-1α Mechanisms.

Hypertension (Dallas, Tex. : 1979)

Kumar S,Wang G,Liu W,Ding W,Dong M,Zheng N,Ye H,Liu J

Wed Aug 01 00:00:00 EDT 2018

SET1A-Mediated Mono-Methylation at K342 Regulates YAP Activation by Blocking Its Nuclear Export and Promotes Tumorigenesis.

Cancer cell

Fang L,Teng H,Wang Y,Liao G,Weng L,Li Y,Wang X,Jin J,Jiao C,Chen L,Peng X,Chen J,Yang Y,Fang H,Han D,Li C,Jin X,Zhang S,Liu Z,Liu M,Wei Q,Liao L,Ge X,Zhao B,Zhou D,Qin HL,Zhou J,Wang P

Mon Jul 09 00:00:00 EDT 2018

Functional interplay between liver X receptor and AMP-activated protein kinase α inhibits atherosclerosis in apolipoprotein E-deficient mice - a new anti-atherogenic strategy.

British journal of pharmacology

Ma C,Zhang W,Yang X,Liu Y,Liu L,Feng K,Zhang X,Yang S,Sun L,Yu M,Yang J,Li X,Hu W,Miao RQ,Zhu Y,Li L,Han J,Chen Y,Duan Y

Tue May 01 00:00:00 EDT 2018

Ubiquitination Regulates the Proteasomal Degradation and Nuclear Translocation of the Fat Mass and Obesity-Associated (FTO) Protein.

Journal of molecular biology

Zhu T,Yong XLH,Xia D,Widagdo J,Anggono V

Fri Feb 02 00:00:00 EST 2018

PADI2-Mediated Citrullination Promotes Prostate Cancer Progression.

Cancer research

Wang L,Song G,Zhang X,Feng T,Pan J,Chen W,Yang M,Bai X,Pang Y,Yu J,Han J,Han B

Wed Nov 01 00:00:00 EDT 2017

Cluster of Differentiation 36 Deficiency Aggravates Macrophage Infiltration and Hepatic Inflammation by Upregulating Monocyte Chemotactic Protein-1 Expression of Hepatocytes Through Histone Deacetylase 2-Dependent Pathway.

Antioxidants & redox signaling

Zhong S,Zhao L,Wang Y,Zhang C,Liu J,Wang P,Zhou W,Yang P,Varghese Z,Moorhead JF,Chen Y,Ruan XZ

Tue Aug 01 00:00:00 EDT 2017

ERK1/2-mediated Cytoplasmic Accumulation of hnRNPK Antagonizes TRAIL-induced Apoptosis through Upregulation of XIAP in H1299 Cells.

Biomedical and environmental sciences : BES

Huang WS,Xu FM,Zeng QZ,Liu XH,Gao XJ,Liu LX

Sat Jul 01 00:00:00 EDT 2017

CRB3 downregulation confers breast cancer stem cell traits through TAZ/β-catenin.

Oncogenesis

Li P,Wang Y,Mao X,Jiang Y,Liu J,Li J,Wang J,Wang R,She J,Zhang J,Yang J,Liu Y,Liu P

Mon Apr 24 00:00:00 EDT 2017

Nuclear retention of the lncRNA SNHG1 by doxorubicin attenuates hnRNPC-p53 protein interactions.

EMBO reports

Shen Y,Liu S,Fan J,Jin Y,Tian B,Zheng X,Fu H

Sat Apr 01 00:00:00 EDT 2017

Regulation of hepatic lipogenesis by the zinc finger protein Zbtb20.

Nature communications

Liu G,Zhou L,Zhang H,Chen R,Zhang Y,Li L,Lu JY,Jiang H,Liu D,Qi S,Jiang YM,Yin K,Xie Z,Shi Y,Liu Y,Cao X,Chen YX,Zou D,Zhang WJ

Wed Mar 22 00:00:00 EDT 2017

TRAIL facilitates cytokine expression and macrophage migration during hypoxia/reoxygenation via ER stress-dependent NF-κB pathway.

Molecular immunology

Jiang Y,Chen X,Fan M,Li H,Zhu W,Chen X,Cao C,Xu R,Wang Y,Ma Y

Wed Feb 01 00:00:00 EST 2017

TRIM52: A nuclear TRIM protein that positively regulates the nuclear factor-kappa B signaling pathway.

Molecular immunology

Fan W,Liu T,Li X,Zhou Y,Wu M,Cui X,Chen H,Qian P

Wed Feb 01 00:00:00 EST 2017

Highly expressed lncRNA CCND2-AS1 promotes glioma cell proliferation through Wnt/β-catenin signaling.

Biochemical and biophysical research communications

Zhang H,Wei DL,Wan L,Yan SF,Sun YH

Sun Jan 22 00:00:00 EST 2017

CRB3 regulates contact inhibition by activating the Hippo pathway in mammary epithelial cells.

Cell death & disease

Mao X,Li P,Wang Y,Liang Z,Liu J,Li J,Jiang Y,Bao G,Li L,Zhu B,Ren Y,Zhao X,Zhang J,Liu Y,Yang J,Liu P

Thu Jan 12 00:00:00 EST 2017

Biallelic SUN5 Mutations Cause Autosomal-Recessive Acephalic Spermatozoa Syndrome.

American journal of human genetics

Zhu F,Wang F,Yang X,Zhang J,Wu H,Zhang Z,Zhang Z,He X,Zhou P,Wei Z,Gecz J,Cao Y

Thu Oct 06 00:00:00 EDT 2016

Dicer interacts with SIRT7 and regulates H3K18 deacetylation in response to DNA damaging agents.

Nucleic acids research

Zhang PY,Li G,Deng ZJ,Liu LY,Chen L,Tang JZ,Wang YQ,Cao ST,Fang YX,Wen F,Xu Y,Chen X,Shi KQ,Li WF,Xie C,Tang KF

Thu May 05 00:00:00 EDT 2016

Set7 facilitates hepatitis C virus replication via enzymatic activity-dependent attenuation of the IFN-related pathway.

Journal of immunology (Baltimore, Md. : 1950)

Han T,Wan Y,Wang J,Zhao P,Yuan Y,Wang L,She Y,Broering R,Lu M,Ye L,Zhu Y

Sun Mar 15 00:00:00 EDT 2015

Novel function of PITH domain-containing 1 as an activator of internal ribosomal entry site to enhance RUNX1 expression and promote megakaryocyte differentiation.

Cellular and molecular life sciences : CMLS

Lu B,Sun X,Chen Y,Jin Q,Liang Q,Liu S,Li Y,Zhou Y,Li W,Huang Z

Sun Feb 01 00:00:00 EST 2015

miR-206 modulates lipopolysaccharide-mediated inflammatory cytokine production in human astrocytes.

Cellular signalling

Duan X,Zohaib A,Li Y,Zhu B,Ye J,Wan S,Xu Q,Song Y,Chen H,Cao S

Thu Jan 01 00:00:00 EST 2015

Protective effect of astaxanthin on liver fibrosis through modulation of TGF-β1 expression and autophagy.

Mediators of inflammation

Shen M,Chen K,Lu J,Cheng P,Xu L,Dai W,Wang F,He L,Zhang Y,Chengfen W,Li J,Yang J,Zhu R,Zhang H,Zheng Y,Zhou Y,Guo C

Thu Dec 18 00:00:00 EST 2014

UDP-glucose dehydrogenase modulates proteoglycan synthesis in articular chondrocytes: its possible involvement and regulation in osteoarthritis.

Arthritis research & therapy

Wen Y,Li J,Wang L,Tie K,Magdalou J,Chen L,Wang H

Wed Dec 03 00:00:00 EST 2014

FHL2-driven molecular network mediated Septin2 knockdown inducing apoptosis in mesangial cell.

Proteomics

Lu Y,Cai G,Cui S,Geng W,Chen D,Wen J,Zhang Y,Zhang F,Xie Y,Fu B,Chen X

Sat Nov 01 00:00:00 EDT 2014

Novel function of the chromosome 7 open reading frame 41 gene to promote leukemic megakaryocyte differentiation by modulating TPA-induced signaling.

Blood cancer journal

Sun X,Lu B,Hu B,Xiao W,Li W,Huang Z

Fri Mar 28 00:00:00 EDT 2014

Proteomic analysis of protein complexes in human SH-SY5Y neuroblastoma cells by using blue-native gel electrophoresis: an increase in lamin A/C associated with heat shock protein 90 in response to 6-hydroxydopamine-induced oxidative stress.

Experimental gerontology

Nakamura M,Morisawa H,Imajoh-Ohmi S,Takamura C,Fukuda H,Toda T

Fri Nov 05 00:00:00 EDT 2010

Proteomic analysis of primary duck hepatocytes infected with duck hepatitis B virus.

Proteome science

Zhao Y,Ben H,Qu S,Zhou X,Yan L,Xu B,Zhou S,Lou Q,Ye R,Zhou T,Yang P,Qu D

Mon Jun 07 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: 70 kDa lamin; lamin A; Lamin A+C mutant; lamin a-c; lamin A/C-like 1; Lamin AC; lamin C; lamin C2; Lamin-A/C; mutant 453W; Mutant lamin A/C; Prelamin-A/C; progerin mutant; Renal carcinoma antigen NY-REN-32; RP11-54H19.1

Gene Aliases: CDCD1; CDDC; CMD1A; CMT2B1; Dhe; EMD2; FPL; FPLD; FPLD2; HGPS; IDC; LDP1; LFP; LGMD1B; LMN1; LMNA; LMNC; LMNL1; PRO1

UniProt ID: (Human) P02545, (Mouse) P48678

Entrez Gene ID: (Human) 4000, (Mouse) 16905, (Rat) 60374

Function(s)