Proteintech

Lamin A/C Polyclonal Antibody

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Lamin A/C Antibody in Immunofluorescence (IF) — This antibody specifically recognizes the heavy chain of Rabbit IgG. It can't detect the The heavy chain of other species. This antibody can be used as the secondary antibody which is specific to the heavy chain of Rabbit IgG.

Lamin A/C Antibody in Western Blot (WB) — Mouse heart tissue were subjected to SDS PAGE followed by western blot with 10298-1-AP (lamin-A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Lamin A/C Antibody in Flow Cytometry (Flow) — 1X10^6 HEK-293T cells were stained with 0.2ug Lamin A/C antibody (10298-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

Lamin A/C Antibody in Immunoprecipitation (IP) — IP result of anti-lamin-A (IP:10298-1-AP, 3ug; Detection:10298-1-AP 1:1000) with A375 cells lysate 800ug.

Product Details

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunocytochemistry (ICC)

1:50-1:500

Immunofluorescence (IF)

1:50-1:500

Immunoprecipitation (IP)

0.5-4.0 µg

Western Blot (WB)

1:1000-1:6000

View 33 publications 33 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species

Human, Mouse, Rat

Published species

Avian, Human, Mouse, Rat

Expression System

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Lamin A/C Fusion Protein Ag0408

Conjugate

Unconjugated

Form

Liquid

Concentration

0.45 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.1% sodium azide

Storage conditions

-20°C

Product Specific Information

This antibody can recognize 4 isoforms of Lamin A/C.

Target Information

Lamins are a class of intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope. These proteins are found in many different cell types in three different forms (A, B, and C). Lamins A and C are alternatively spliced versions of the LMNA gene. The LMNA gene has been linked to many disorders of the muscular system, nervous system, and the fat distributions systems including: Emery-Dreifuss muscular dystrophy, Dunnigan-type familial partial lipodystrophy (FPLD), limb-girdle muscular dystrophy (LGMD1B), dilated cardiomyopathy (CMD1A), axonal neuropathy (Charcot-Marie-Tooth disease; CMT2B1), and mandibuloacral dysplasia (MAD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 70 kDa lamin; lamin A; Lamin A+C mutant; lamin a-c; lamin A/C-like 1; Lamin AC; lamin C; lamin C2; Lamin-A/C; mutant 453W; Mutant lamin A/C; Prelamin-A/C; progerin mutant; Renal carcinoma antigen NY-REN-32; RP11-54H19.1

Gene Aliases: CDCD1; CDDC; CMD1A; CMT2B1; Dhe; EMD2; FPL; FPLD; FPLD2; HGPS; IDC; LDP1; LFP; LGMD1B; LMN1; LMNA; LMNC; LMNL1; PRO1

UniProt ID: (Human) P02545, (Mouse) P48678

Entrez Gene ID: (Human) 4000, (Mouse) 16905, (Rat) 60374

Molecular Function: cytoskeletal protein intermediate filament structural protein