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Immunofluorescent analysis of Lamin A/C (green) in untreated U2-OS cells (A) or HeLa cells (B). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 0.3% BSA for 15 minutes at room temperature. Cells were then probed with a mouse monoclonal antibody recognizing Lamin A/C (Product # MA3-1000), at a dilution of 1:100 for at least 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody (Product# 35503) at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product# 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.
|Tested species reactivity||Bovine, Human, Pig|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Porcine lamin preparation.|
|Storage buffer||tissue culture supernatant|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:100|
|Western Blot (WB)||1:100 - 1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA3-1000 detects lamin A/C from human, bovine, porcine and canine samples.
MA3-1000 has been successfully used in Western blot, immunofluorescence and immunohistochemistry procedures. By Western blot, this antibody detects ~ 70 and 65 kDa proteins corresponding to lamin A and C, respectively, from HeLa cell extract. This antibody can also be used for immunofluorescence and immunohistochemistry for subcellular localization experiments. MA3-1000 has been widely used for detection of lamin A/C control in siRNA experiments.
The MA3-1000 antigen is porcine lamin preparation.
Lamins are a class of intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope. These proteins are found in many different cell types in three different forms (A, B, and C). Lamins A and C are alternatively spliced versions of the LMNA gene. The LMNA gene has been linked to many disorders of the muscular system, nervous system, and the fat distributions systems including: Emery-Dreifuss muscular dystrophy, Dunnigan-type familial partial lipodystrophy (FPLD), limb-girdle muscular dystrophy (LGMD1B), dilated cardiomyopathy (CMD1A), axonal neuropathy (Charcot-Marie-Tooth disease; CMT2B1), and mandibuloacral dysplasia (MAD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Perinuclear localization of the HIV-1 regulatory protein Vpr is important for induction of G2-arrest.
MA3-1000 was used in immunocytochemistry to study the role of the HIV regulatory protein Vpr in G2 arrest
|Sörgel S,Fraedrich K,Votteler J,Thomas M,Stamminger T,Schubert U||Virology (432:444)||2012|
Identification of RANBP16 and RANBP17 as novel interaction partners for the bHLH transcription factor E12.
MA3-1000 was used in western blot to investigate the effect of RANBP 16 and 17 on bHLH transcription factor E12 function
|Lee JH,Zhou S,Smas CM||Journal of cellular biochemistry (111:195)||2010|
Myosin Vb localises to nucleoli and associates with the RNA polymerase I transcription complex.
MA3-1000 was used in western blot to study the localization and functions of myosin Va and Vb
|Lindsay AJ,McCaffrey MW||Cell motility and the cytoskeleton (66:1057)||2009|
70 kDa lamin; lamin A/C-like 1; lamin-A/C; Prelamin-A/C; renal carcinoma antigen NY-REN-32
BOS_2775; CDCD1; CDDC; CMD1A; CMT2B1; EMD2; FPL; FPLD; FPLD2; HGPS; IDC; LDP1; LFP; LGMD1B; LMN1; LMNA; LMNC; LMNL1; PRO1