|Immunohistochemistry (Frozen) (IHC (F))||1:100|
|Western Blot (WB)||1:100 - 1:2000|
|Western Blot (WB)||See 6 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Tested Species reactivity||Bovine, Human, Pig|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Porcine lamin preparation.|
|Storage buffer||tissue culture supernatant|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA3-1000 detects lamin A/C from human, bovine, porcine and canine samples.
MA3-1000 has been successfully used in Western blot, immunofluorescence and immunohistochemistry procedures. By Western blot, this antibody detects ~ 70 and 65 kDa proteins corresponding to lamin A and C, respectively, from HeLa cell extract. This antibody can also be used for immunofluorescence and immunohistochemistry for subcellular localization experiments. MA3-1000 has been widely used for detection of lamin A/C control in siRNA experiments.
The MA3-1000 antigen is porcine lamin preparation.
Lamins are a class of intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope. These proteins are found in many different cell types in three different forms (A, B, and C). Lamins A and C are alternatively spliced versions of the LMNA gene. The LMNA gene has been linked to many disorders of the muscular system, nervous system, and the fat distributions systems including: Emery-Dreifuss muscular dystrophy, Dunnigan-type familial partial lipodystrophy (FPLD), limb-girdle muscular dystrophy (LGMD1B), dilated cardiomyopathy (CMD1A), axonal neuropathy (Charcot-Marie-Tooth disease; CMT2B1), and mandibuloacral dysplasia (MAD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 70 kDa lamin; lamin A/C-like 1; lamin-A/C; Prelamin-A/C; renal carcinoma antigen NY-REN-32
Gene Aliases: BOS_2775; CDCD1; CDDC; CMD1A; CMT2B1; EMD2; FPL; FPLD; FPLD2; HGPS; IDC; LDP1; LFP; LGMD1B; LMN1; LMNA; LMNC; LMNL1; PRO1
UniProt ID: (Human) P02545
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