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|Tested species reactivity||Bovine, Dog, Human, Mouse, Sheep, Rabbit, Rat|
|Published species reactivity||Bovine, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Purified rat liver lamin.|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||1:100-1:200|
|Immunohistochemistry (Frozen) (IHC (F))||1:100-1:200|
|Western Blot (WB)||1:100-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
MA1-06103 detects lamin B in human, mouse, rat, bovine, ovine, rabbit, zebrafish, and canine samples.
MA1-06103 has sucessfully been used in immunocytochemistry, immunohistochemistry, ELISA, and flow cytometry procedures.
The MA1-06103 immunogen is purified rat liver lamins.
Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Comparative 2D-DIGE proteomic analysis of bovine mammary epithelial cells during lactation reveals protein signatures for lactation persistency and milk yield.
MA1-06103 was used in western blot to use 2D-DIGE and mass spectrometry to identify the protein changes in mammary epithelial cells
|Janjanam J,Singh S,Jena MK,Varshney N,Kola S,Kumar S,Kaushik JK,Grover S,Dang AK,Mukesh M,Prakash BS,Mohanty AK||PloS one (9:null)||2014|
|Not Applicable||Not Cited||
Receptor tyrosine kinase ErbB2 translocates into mitochondria and regulates cellular metabolism.
MA1-06103 was used in western blot to study the function of mitochondrial ErbB2
|Ding Y,Liu Z,Desai S,Zhao Y,Liu H,Pannell LK,Yi H,Wright ER,Owen LB,Dean-Colomb W,Fodstad O,Lu J,LeDoux SP,Wilson GL,Tan M||Nature communications (3:null)||2012|
ADLD; lamin B1; Lamin-B1; LMN; LMN2; LMNB; MGC111419
ADLD; BOS_7828; LMN; LMN2; LMNB; LMNB1