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Immunocytochemistry analysis of Lamin B1 in U2OS cells. Cells fixed with 4% paraformaldehyde were permeabilized with 0.1% Triton X-100 (Product #28314) in PBS for 15 minutes at room temperature and blocked with 2% BSA in PBST (from Product # 37525) for 30 minutes at room temperature. Cells were treated with Peroxidase Suppressor (Product # 35000), probed with a Lamin B1 polyclonal antibody (Product # PA5-19468) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBST, and incubated with an HRP-conjugated goat anti-rabbit IgG (H+L) secondary antibody (Product # 31466) at a dilution of 1:1000 for 30 minutes at room temperature. Chromogenic detection was performed using Metal Enhanced DAB Substrate Kit (Product # 34065). Images were taken on a Zeiss Axio Observer microscope at 20X magnification (x1.6 Optovar ~ 32X).
|Tested species reactivity||Deer, Human, Mouse, Rat, Xenopus|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Mouse Lamin B1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1 µg/ml|
|Immunofluorescence (IF)||1 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||Assay-Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1 µg/ml|
|Western Blot (WB)||0.1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat mediated antigen retrieval recommended prior to tissue staining.
This antibody is predicted to react with chicken and zebrafish based on sequence homology.
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Alternative splicing results in transcript variants and a duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy .
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Curcumin induces oxidation-dependent cell cycle arrest mediated by SIRT7 inhibition of rDNA transcription in human aortic smooth muscle cells.
PA5-19468 was used in western blot to determine if curcumin can diminish/prevent the development of cardiovascular pathologies
|Lewinska A,Wnuk M,Grabowska W,Zabek T,Semik E,Sikora E,Bielak-Zmijewska A||Toxicology letters (233:227)||2015|
Acute pulmonary dose-responses to inhaled multi-walled carbon nanotubes.
PA5-19468 was used in immunohistochemistry to study the pulmonary toxicity of inhaled multi-walled carbon nanotubes in a murine model
|Porter DW,Hubbs AF,Chen BT,McKinney W,Mercer RR,Wolfarth MG,Battelli L,Wu N,Sriram K,Leonard S,Andrew M,Willard P,Tsuruoka S,Endo M,Tsukada T,Munekane F,Frazer DG,Castranova V||Nanotoxicology (7:1179)||2013|
ADLD; lamin B1; lamin-B1; LMN; LMN2; LMNB; MGC111419
ADLD; lamin-b; Lamin-L(I); LMN; LMN2; LMNB; LMNB1