Immunofluorescence analysis of Lamin B1 was performed using 70% confluent log phase NIH/3T3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Lamin B1 Rabbit Polyclonal Antibody (PA519468) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Dog, Deer, Human, Mouse, Non-human primate, Rat, Xenopus|
|Published species reactivity||Human, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Mouse Lamin B1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||Assay-Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1 µg/ml|
|Western Blot (WB)||0.5 - 1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat mediated antigen retrieval recommended prior to tissue staining.
This antibody is predicted to react with chicken and zebrafish based on sequence homology.
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Alternative splicing results in transcript variants and a duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy .
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Farnesoid X receptor-dependent and -independent pathways mediate the transcriptional control of human fibroblast growth factor 19 by vitamin A.
PA5-19468 was used in western blot to analyze how transcription control of human fibroblast growth factor factor 19 by vitamin A is regulated by farnesoid X receptor independent and dependent pathways
|Jahn D,Sutor D,Dorbath D,Weiß J,Götze O,Schmitt J,Hermanns HM,Geier A||Biochimica et biophysica acta (1859:381)||2016|
Curcumin induces oxidation-dependent cell cycle arrest mediated by SIRT7 inhibition of rDNA transcription in human aortic smooth muscle cells.
PA5-19468 was used in western blot to determine if curcumin can diminish/prevent the development of cardiovascular pathologies
|Lewinska A,Wnuk M,Grabowska W,Zabek T,Semik E,Sikora E,Bielak-Zmijewska A||Toxicology letters (233:227)||2015|
|Not Applicable||Not Cited||
A novel role for histone deacetylase 6 in the regulation of the tolerogenic STAT3/IL-10 pathway in APCs.
PA5-19468 was used in western blot to study the effects of HDAC6 modulation using antigen presenting cells
|Cheng F,Lienlaf M,Wang HW,Perez-Villarroel P,Lee C,Woan K,Rock-Klotz J,Sahakian E,Woods D,Pinilla-Ibarz J,Kalin J,Tao J,Hancock W,Kozikowski A,Seto E,Villagra A,Sotomayor EM||Journal of immunology (Baltimore, Md. : 1950) (193:2850)||2014|
Acute pulmonary dose-responses to inhaled multi-walled carbon nanotubes.
PA5-19468 was used in immunohistochemistry to study the pulmonary toxicity of inhaled multi-walled carbon nanotubes in a murine model
|Porter DW,Hubbs AF,Chen BT,McKinney W,Mercer RR,Wolfarth MG,Battelli L,Wu N,Sriram K,Leonard S,Andrew M,Willard P,Tsuruoka S,Endo M,Tsukada T,Munekane F,Frazer DG,Castranova V||Nanotoxicology (7:1179)||2013|