Immunofluorescent analysis of Lamin B2 in HeLa cells showing staining in the nuclear envelope using a Lamin B2 monoclonal antibody (Product # MA5-17274) at a dilution of 1:1000 (red). Cells were fixed in 2% paraformaldehyde for 30 min. Green: alpha Tubulin antibody at a dilution of 1:1000. Blue: Hoechst 33342.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Recombinant fragment contains a sequence corresponding to a region within amino acids 277 and 600 of Lamin B2|
|Storage buffer||PBS, pH 7|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-17274 targets Lamin B2 in ICC, IF, IF, IHC (P), and WB applications and shows reactivity with Human samples.
The MA5-17274 immunogen is recombinant fragment contains a sequence corresponding to a region within amino acids 277 and 600 of Lamin B2.
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.