|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human Laminin(1). Monoclonal antibody was obtained by fusing the mouse myeloma cell-line P3U1 with spleen cells of BALB/c mouse after immunization with human Laminin (|
|Purification||Ion-exchange chromatography, Ammonium sulfate precipitation|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunofluorescence (IF)||See 1 publications below|
Extracellular matrix (ECM) surrounds and separates most tissue compartments, determines tissue architecture and dynamically interacts with its surrounding cells. The basement membrane is a part of the ECM and is composed of collagens, proteoglycans, elastin and several glycoproteins of which laminin is the most abundant. Laminins are large heterotrimeric, noncollagenous glycoproteins composed of alpha, beta, and gamma chains Dystroglycan is a major laminin receptor in muscle and epithelia, and nidogen is a high-affinity laminin-binding protein important for basement-membrane assembly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Human||Not Cited||Visualization of spindle behavior using confocal microscopy.||Marcus AI||Methods in molecular medicine (137:125)||2007|