|Tested species reactivity||Human, Mouse, Sheep, Rabbit, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Laminin isolated from mouse EHS tumor.|
|Contains||0.05% sodium azide|
|Storage Conditions||-20°C or -80°C if preferred|
|Tested Applications||Dilution *|
|Functional Assay (FN)||Assay-Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:500-1:2000|
|Immunohistochemistry (Paraffin) (IHC (P))||1:500-1:2000|
|Western Blot (WB)||1:100-1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
The antibody functionally inhibits Laminin in mouse and rat.
It binds to Laminin and inhibits most, if not all, of its cell adhesion and growth promotive properties.
Immunostaining is enhanced by antigen retrieval with pepsin, especially paraffin tissue.Suggested positive control: basement membranes (blood vessels of any tissue, tumors, nerve).
Laminin antibodies are widely used to label blood vessels and basement membranes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A semi-interpenetrating network of polyacrylamide and recombinant basement membrane allows pluripotent cell culture in a soft, ligand-rich microenvironment.
PA1-16730 was used in immunocytochemistry to develop a method to generate semi-interpenetrating networks of polyacrylamide and recombinant basement membrane to culture human embryonic stem cells
|Price AJ,Huang EY,Sebastiano V,Dunn AR||Biomaterials (121:179)||2017|