Invitrogen

Laminin Monoclonal Antibody (A5)

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Antibody Testing Data (4)

Laminin Antibody in Immunocytochemistry (ICC/IF)

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Laminin Antibody (MA1-06100) in ICC/IF

Immunofluorescence analysis of laminin was performed using 70% confluent log phase A431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Laminin Monoclonal Antibody (Product # MA1-06100) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rat IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of... View More

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Laminin Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Immunohistochemistry analysis of Laminin beta 2 gamma 1 in frozen sections from pig striated skeletal muscle (methanol fixed) using a Laminin beta-2/gamma-1 Monoclonal Antibody (A5) (Product # MA1-06100), showing the localization of laminin in the connective tissue and in basement membranes surrounding the myofibrils.

Product Details

MA1-06100

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 15 publications 15 publications

Immunohistochemistry (Frozen) (IHC (F))

1:25-1:200

Immunocytochemistry (ICC/IF)

5 µg/mL

View 1 publication 1 publication

ELISA (ELISA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Pig

Published species

Human, Mouse, Rat, Rhesus monkey

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

Immunogen

Laminin preparation from the EHS mouse tumor.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_559896

Product Specific Information

MA1-06100 detects laminin in human and mouse samples.

MA1-06100 has sucessfully been used in immunocytochemistry and immunohistochemistry procedures.

The MA1-06100 immunogen is a laminin preparation from the EHS mouse tumor.

Target Information

Laminin antibodies are widely used to label blood vessels and basement membranes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A 50 kdyne contusion spinal cord injury with or without the drug SS-31 was not associated with major changes in muscle mass or gene expression 14 d after injury in young male mice.

Physiological reports

Graham ZA,DeBerry JJ,Cardozo CP,Bamman MM

MA1-06100 was used in Immunohistochemistry to determine if mitochondrial-targeting peptide SS-31 could prevent muscle loss after spinal cord injury.

Mon Feb 01 00:00:00 EST 2021

Skeletal muscle transcriptional networks linked to type I myofiber grouping in Parkinson's disease.

Journal of applied physiology (Bethesda, Md. : 1985)

Lavin KM,Sealfon SC,McDonald MN,Roberts BM,Wilk K,Nair VD,Ge Y,Lakshman Kumar P,Windham ST,Bamman MM

MA1-06100 was used in Immunohistochemistry to provide insight into potential mechanistic roles of skeletal muscle in signaling to promote its survival in PD.

Sat Feb 01 00:00:00 EST 2020

Cell-Based Strain Remodeling of a Nonfibrous Matrix as an Organizing Principle for Vasculogenesis.

Cell reports

Rüdiger D,Kick K,Goychuk A,Vollmar AM,Frey E,Zahler S

MA1-06100 was used in Immunocytochemistry to identify a mechanism that allows cells to form networks by mechanically reorganizing and stiffening their extracellular matrix, independent of chemical guidance cues.

Tue Aug 11 00:00:00 EDT 2020

Disease-modifying bioactivity of intravenous cardiosphere-derived cells and exosomes in mdx mice.

JCI insight

Rogers RG,Fournier M,Sanchez L,Ibrahim AG,Aminzadeh MA,Lewis MI,Marbán E

MA1-06100 was used in Immunohistochemistry to demonstrate regenerative responses of cardiac and skeletal muscle tissue to intravenous cardiosphere-derived cells and exosomes in mdx mice.

Thu Apr 04 00:00:00 EDT 2019

The role of pericytic laminin in blood brain barrier integrity maintenance.

Scientific reports

Gautam J,Zhang X,Yao Y

MA106100 was used in western blot to examine the function of pericyte-derived laminin in vascular integrity

Thu Nov 03 00:00:00 EDT 2016

Anti-DNA autoantibodies initiate experimental lupus nephritis by binding directly to the glomerular basement membrane in mice.

Kidney international

Krishnan MR,Wang C,Marion TN

MA1-06100 was used in ELISA to test if direct binding of anti-DNA antibody to the glomerular basement membrane important in experimental lupus nephritis

Sun Jul 01 00:00:00 EDT 2012

Deletion of muscle GRP94 impairs both muscle and body growth by inhibiting local IGF production.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Barton ER,Park S,James JK,Makarewich CA,Philippou A,Eletto D,Lei H,Brisson B,Ostrovsky O,Li Z,Argon Y

MA1-06100 was used in immunohistochemistry to study the role of local IGF production in skeletal muscle and body growth using GRP94 knockout mice

Sat Sep 01 00:00:00 EDT 2012

Blood-brain barrier disruption in CCL2 transgenic mice during pertussis toxin-induced brain inflammation.

Fluids and barriers of the CNS

Schellenberg AE,Buist R,Del Bigio MR,Toft-Hansen H,Khorooshi R,Owens T,Peeling J

MA1-06100 was used in immunohistochemistry to study the effect of transgenic overexpression of CCL2 on blood-brain barrier integrity during pertussis toxin-induced brain inflammation

Mon Apr 30 00:00:00 EDT 2012

Omega 3 fatty acids supplementation has an ameliorative effect in experimental ulcerative colitis despite increased colonic neutrophil infiltration.

Revista espanola de enfermedades digestivas : organo oficial de la Sociedad Espanola de Patologia Digestiva

Varnalidis I,Ioannidis O,Karamanavi E,Ampas Z,Poutahidis T,Taitzoglou I,Paraskevas G,Botsios D

MA1-06100 was used in immunohistochemistry to study the efficacy of omega 3 fatty acids in the treatment of experimental ulcerative colitis

Sat Oct 01 00:00:00 EDT 2011

Decellularized rhesus monkey kidney as a three-dimensional scaffold for renal tissue engineering.

Tissue engineering. Part A

Nakayama KH,Batchelder CA,Lee CI,Tarantal AF

MA1-06100 was used in immunohistochemistry to study the use of decellularized rhesus monkey kidney as a three-dimensional scaffold in renal tissue engineering

Thu Jul 01 00:00:00 EDT 2010

Diaphragm displays early and progressive functional deficits in dysferlin-deficient mice.

Muscle & nerve

Barton ER,Wang BJ,Brisson BK,Sweeney HL

MA1-06100 was used in immunohistochemistry to study the potential use of diaphragm muscle for measuring functional deficits in mice deficient in dysferlin and as a model for therapeutic interventions

Thu Jul 01 00:00:00 EDT 2010

Regulation of muscle mass by follistatin and activins.

Molecular endocrinology (Baltimore, Md.)

Lee SJ,Lee YS,Zimmers TA,Soleimani A,Matzuk MM,Tsuchida K,Cohn RD,Barton ER

MA1-06100 was used in immunohistochemistry to study the role of follistatin and activins in regulating muscle mass

Fri Oct 01 00:00:00 EDT 2010

Coupling of neural activity to blood flow in olfactory glomeruli is mediated by astrocytic pathways.

Neuron

Petzold GC,Albeanu DF,Sato TF,Murthy VN

MA1-06100 was used in immunohistochemistry to study the role of astrocytic pathways in coupling neural activity to blood flow in olfactory glomeruli

Thu Jun 26 00:00:00 EDT 2008

Expression profiling reveals heightened apoptosis and supports fiber size economy in the murine muscles of mastication.

Physiological genomics

Evans M,Morine K,Kulkarni C,Barton ER

MA1-06100 was used in immunohistochemistry to compare the expression profiles of masticatory and limb muscles in female mice

Wed Sep 17 00:00:00 EDT 2008

Misty somites, a maternal effect gene identified by transposon-mediated insertional mutagenesis in zebrafish that is essential for the somite boundary maintenance.

Developmental biology

Kotani T,Kawakami K

MA1-06100 was used in immunohistochemistry to study the role of the maternal effect gene misty somites in the maintenance of the somite boundary in zebrafish

Tue Apr 15 00:00:00 EDT 2008

Altered renal morphology in transgenic mice with cholecystokinin overexpression.

Transgenic research

Aunapuu M,Roosaar P,Järveots T,Kurrikoff K,Kõks S,Vasar E,Arend A

MA1-06100 was used in immunohistochemistry to study the renal morphology of transgenic mice overexpressing cholecystokinin

Mon Dec 01 00:00:00 EST 2008

Expression and distribution of tissue transglutaminase in normal and injured rat cornea.

Current eye research

Zhang W,Shiraishi A,Suzuki A,Zheng X,Kodama T,Ohashi Y

MA1-06100 was used in immunohistochemistry to study the expression of tissue transglutaminase and ECM proteins during corneal wound healing

Thu Jan 01 00:00:00 EST 2004

Globose basal cells are required for reconstitution of olfactory epithelium after methyl bromide lesion.

The Journal of comparative neurology

Jang W,Youngentob SL,Schwob JE

MA1-06100 was used in immunohistochemistry to study the requirement for globose basal cells for the regeneration of olfactory epithelium following damage by methyl bromide treatment

Mon May 19 00:00:00 EDT 2003

Bioinformatics

Protein Aliases: basement membrane protein; dystrophia muscularis; LAMB1; basement membrane protein; Laminin A chain; Laminin B1 chain; laminin B1 subunit 1; Laminin B2 chain; laminin beta-1 chain; Laminin M chain; laminin S; Laminin subunit alpha-1; Laminin subunit alpha-2; Laminin subunit beta-1; Laminin subunit gamma-1; laminin, alpha 1; laminin, alpha 2; laminin, beta 1; laminin, beta 2; laminin, gamma 1 (formerly LAMB2); Laminin-1 subunit alpha; Laminin-1 subunit beta; Laminin-1 subunit gamma; Laminin-10 subunit beta; Laminin-10 subunit gamma; Laminin-11 subunit gamma; Laminin-12 subunit alpha; Laminin-12 subunit beta; Laminin-2 subunit alpha; Laminin-2 subunit beta; Laminin-2 subunit gamma; Laminin-3 subunit alpha; Laminin-3 subunit gamma; Laminin-4 subunit alpha; Laminin-4 subunit gamma; Laminin-6 subunit beta; Laminin-6 subunit gamma; Laminin-7 subunit gamma; Laminin-8 subunit beta; Laminin-8 subunit gamma; Laminin-9 subunit gamma; Merosin heavy chain; S-LAM alpha; S-LAM gamma; S-laminin subunit alpha; S-laminin subunit gamma

Gene Aliases: 5830440B04; AA408497; C77966; C80098; C81607; CLM; D130003D08Rik; dy; LAMA; Lama-1; LAMA1; LAMA2; Lamb-1; LAMB1; Lamb1-1; LAMB2; LAMC1; LAMM; LIS5; mer; merosin; mKIAA4087; PTBHS; S-LAM-alpha

UniProt ID: (Human) P25391, (Human) P24043, (Human) P07942, (Human) P11047, (Mouse) P19137, (Mouse) Q60675, (Mouse) P02469

Entrez Gene ID: (Human) 284217, (Pig) 100625589, (Human) 3908, (Pig) 100154420, (Human) 3912, (Pig) 396707, (Human) 3915, (Pig) 100514785, (Mouse) 16772, (Mouse) 16773, (Mouse) 16777, (Mouse) 226519