|Tested species reactivity||Hamster, Human, Mouse, Pig|
|Published species reactivity||Mouse|
|Host / Isotype||Rat / IgG1, kappa|
|Immunogen||Murine EHS laminin preparation|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||2 µg/mL|
|Immunohistochemistry (Paraffin) (IHC (P))||2 µg/mL|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
MA5-14657 targets Laminin B1/Beta1 in IF, IHC (P, F), IP, and WB applications and shows reactivity with Hamster, Human, mouse, and Porcine samples.
The MA5-14657 immunogen is mouse EHS laminin preparation.
Laminins are large heterotrimeric, noncollagenous glycoproteins composed of alpha, beta, and gamma chains. They are ubiquitously present in basement membrane (BM) along with entactin/nidogen (EN), collagen type IV (CIV), and large heparan sulfate proteoglycan (HSPG), which interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Deletion of the laminin alpha4 chain leads to impaired microvessel maturation.
MA5-14657 was used in immunohistochemistry to study the essential role of laminin alpha4 chain in microvessel maturation
|Thyboll J,Kortesmaa J,Cao R,Soininen R,Wang L,Iivanainen A,Sorokin L,Risling M,Cao Y,Tryggvason K||Molecular and cellular biology (22:1194)||2002|