|Tested species reactivity||Human, Mouse|
|Published species reactivity||Rat, Mouse, Not Applicable, Rhesus monkey|
|Host / Isotype||Rat / IgG2a, kappa|
|Immunogen||Murine EHS laminin preparation|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||2 µg/mL|
|Immunohistochemistry (Paraffin) (IHC (P))||2 µg/mL|
|Immunoprecipitation (IP)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-14649 targets Laminin B2/gamma1 in IF, IHC (P, F), and IP applications and shows reactivity with Human and mouse samples.
The MA5-14649 immunogen is mouse EHS laminin preparation.
Laminins are large heterotrimeric, noncollagenous glycoproteins composed of alpha, beta, and gamma chains. They are ubiquitously present in basement membrane (BM) along with entactin/nidogen (EN), collagen type IV (CIV), and large heparan sulfate proteoglycan (HSPG), which interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Deletion of muscle GRP94 impairs both muscle and body growth by inhibiting local IGF production.
MA5-14649 was used in immunohistochemistry to study the role of local IGF production in skeletal muscle and body growth using GRP94 knockout mice
|Barton ER,Park S,James JK,Makarewich CA,Philippou A,Eletto D,Lei H,Brisson B,Ostrovsky O,Li Z,Argon Y||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (26:3691)||2012|
Blood-brain barrier disruption in CCL2 transgenic mice during pertussis toxin-induced brain inflammation.
MA5-14649 was used in immunohistochemistry to study the effect of transgenic overexpression of CCL2 on blood-brain barrier integrity during pertussis toxin-induced brain inflammation
|Schellenberg AE,Buist R,Del Bigio MR,Toft-Hansen H,Khorooshi R,Owens T,Peeling J||Fluids and barriers of the CNS (9:null)||2012|
Omega 3 fatty acids supplementation has an ameliorative effect in experimental ulcerative colitis despite increased colonic neutrophil infiltration.
MA5-14649 was used in immunohistochemistry to study the efficacy of omega 3 fatty acids in the treatment of experimental ulcerative colitis
|Varnalidis I,Ioannidis O,Karamanavi E,Ampas Z,Poutahidis T,Taitzoglou I,Paraskevas G,Botsios D||Revista espanola de enfermedades digestivas : organo oficial de la Sociedad Espanola de Patologia Digestiva (103:511)||2011|
Regulation of muscle mass by follistatin and activins.
MA5-14649 was used in immunohistochemistry to study the role of follistatin and activins in regulating muscle mass
|Lee SJ,Lee YS,Zimmers TA,Soleimani A,Matzuk MM,Tsuchida K,Cohn RD,Barton ER||Molecular endocrinology (Baltimore, Md.) (24:1998)||2010|
Decellularized rhesus monkey kidney as a three-dimensional scaffold for renal tissue engineering.
MA5-14649 was used in immunohistochemistry to study the use of decellularized rhesus monkey kidney as a three-dimensional scaffold in renal tissue engineering
|Nakayama KH,Batchelder CA,Lee CI,Tarantal AF||Tissue engineering. Part A (16:2207)||2010|
Altered renal morphology in transgenic mice with cholecystokinin overexpression.
MA5-14649 was used in immunohistochemistry to study the renal morphology of transgenic mice overexpressing cholecystokinin
|Aunapuu M,Roosaar P,Järveots T,Kurrikoff K,Kõks S,Vasar E,Arend A||Transgenic research (17:1079)||2008|
Expression profiling reveals heightened apoptosis and supports fiber size economy in the murine muscles of mastication.
MA5-14649 was used in immunohistochemistry to compare the expression profiles of masticatory and limb muscles in female mice
|Evans M,Morine K,Kulkarni C,Barton ER||Physiological genomics (35:86)||2008|
Coupling of neural activity to blood flow in olfactory glomeruli is mediated by astrocytic pathways.
MA5-14649 was used in immunohistochemistry to study the role of astrocytic pathways in coupling neural activity to blood flow in olfactory glomeruli
|Petzold GC,Albeanu DF,Sato TF,Murthy VN||Neuron (58:897)||2008|
Misty somites, a maternal effect gene identified by transposon-mediated insertional mutagenesis in zebrafish that is essential for the somite boundary maintenance.
MA5-14649 was used in immunohistochemistry to study the role of the maternal effect gene misty somites in the maintenance of the somite boundary in zebrafish
|Kotani T,Kawakami K||Developmental biology (316:383)||2008|
Melatonin protects against epirubicin-induced cardiotoxicity.
MA5-14649 was used in immunohistochemistry to study the role of melatonin in protecting against epirubicin-induced cardiotoxicity
|Guven A,Yavuz O,Cam M,Ercan F,Bukan N,Comunoglu C||Acta histochemica (109:52)||2007|
Investigation of the effects of heparin and low molecular weight heparin on E-cadherin and laminin expression in rat pregnancy by immunohistochemistry.
MA5-14649 was used in immunohistochemistry to investigate E-cadherin and laminin expression in rat placental and decidual tissues
|Erden O,Imir A,Guvenal T,Muslehiddinoglu A,Arici S,Cetin M,Cetin A||Human reproduction (Oxford, England) (21:3014)||2006|
Expression and distribution of tissue transglutaminase in normal and injured rat cornea.
MA5-14649 was used in immunohistochemistry to study the expression of tissue transglutaminase and ECM proteins during corneal wound healing
|Zhang W,Shiraishi A,Suzuki A,Zheng X,Kodama T,Ohashi Y||Current eye research (28:37)||2004|
Globose basal cells are required for reconstitution of olfactory epithelium after methyl bromide lesion.
MA5-14649 was used in immunohistochemistry to study the requirement for globose basal cells for the regeneration of olfactory epithelium following damage by methyl bromide treatment
|Jang W,Youngentob SL,Schwob JE||The Journal of comparative neurology (460:123)||2003|
|Not Applicable||Not Cited||
Anti-DNA autoantibodies initiate experimental lupus nephritis by binding directly to the glomerular basement membrane in mice.
MA5-14649 was used in ELISA to test if direct binding of anti-DNA antibody to the glomerular basement membrane important in experimental lupus nephritis
|Krishnan MR,Wang C,Marion TN||Kidney international (82:184)||2012|