|Tested species reactivity||Bovine, Fish, Human, Mouse, Rat|
|Published species reactivity||Bovine, Mouse, Human|
|Host / Isotype||Rat / IgG2a|
|Immunogen||High molecular mass material derived from the Engelbreth-Holm-Swarm (EHS) tumor matrix containing laminin, entactin and HSPG.|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:100-1:500|
|Immunohistochemistry (Paraffin) (IHC (P))||1:25-1:200|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:100-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 5 publications below|
MA1-06821 detects large heparan sulphate proteoglycan in human, mouse, bovine, cod, wolffish, and rat samples.
MA1-06821 has sucessfully been used in Western blotting, immunocytochemistry, immunoprecipitation, and immunohistochemistry procedures. By Western blot it detects a >400 kDa band representing large HSPG.
The MA1-06821 immunogen is a high molecular mass material derived from the Engelbreth-Holm-Swarm (EHS) tumor matrix containing laminin, entactin and HSPG.
Proteoglycans are macromolecules consisting of a variety of core proteins with covalently attached one or several polysaccharide chains of the glycosaminoglycan type (heparan sulphate, heparin, chondroitin sulphate, dermatan sulphate or keratan sulphate). At least two forms of basement membrane heparan sulphate proteoglycan (HSPG) have been identified. One with a large core protein (>400 kD) and one with a small core protein (~30 kD). The large HSPG is probably the most abundant basement membrane proteoglycan. It is located predominantly in the lamina lucida, where it forms clustered aggregates and interacts with other basement membrane components to form the matrix. In addition, it also plays a critical role in attachment of cells to the basal membrane via integrin receptors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Liver aging and pseudocapillarization in a Werner syndrome mouse model.
MA1-06821 was used in immunohistochemistry to study the accelerated age-related hepatic changes observed in a murine model of Werner syndrome
|Cogger VC,Svistounov D,Warren A,Zykova S,Melvin RG,Solon-Biet SM,O'Reilly JN,McMahon AC,Ballard JW,De Cabo R,Le Couteur DG,Lebel M||The journals of gerontology. Series A, Biological sciences and medical sciences (69:1076)||2014|
LARGE glycans on dystroglycan function as a tunable matrix scaffold to prevent dystrophy.
MA1-06821 was used in immunohistochemistry to study the role of dystroglycan LARGE glycans in the normal functioning of skeletal muscle
|Goddeeris MM,Wu B,Venzke D,Yoshida-Moriguchi T,Saito F,Matsumura K,Moore SA,Campbell KP||Nature (503:136)||2013|
DOT1L regulates dystrophin expression and is critical for cardiac function.
MA1-06821 was used in immunohistochemistry to study the role of DOT1L in regulating dystrophin expression and cardiac function
|Nguyen AT,Xiao B,Neppl RL,Kallin EM,Li J,Chen T,Wang DZ,Xiao X,Zhang Y||Genes and development (25:263)||2011|
The effect of bovine endosteum-derived particles on the proliferation of human mesenchymal stem cells.
MA1-06821 was used in immunohistochemistry to evaluate the usefulness of bovine endosteum-derived particles in stem cell culture
|Nigro J,White JF,Ramshaw JA,Haylock DN,Nilsson SK,Werkmeister JA||Biomaterials (31:5689)||2010|
Synthesis of syndecan-1 by skeletal muscle cells is an early response to infection with Trichinella spiralis but is not essential for nurse cell development.
MA1-06821 was used in immunohistochemistry to study the synthesis of syndecan-1 by skeletal muscle cells in response to Trichinella spiralis infection
|Beiting DP,Park PW,Appleton JA||Infection and immunity (74:1941)||2006|