Invitrogen

Lass5 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

1 Published Figure

1 Reference

View all (8) Lass5 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Lass5 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 5

Lass5 Antibody (PA5-20570) in ICC/IF

Immunofluorescence of LASS5 in Human Brain cells with Lass5 Polyclonal Antibody (Product # PA5-20570) at 20 µg/mL.

Lass5 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Lass5 Antibody — Fig. 4. Dexamethasone-activated hepatic ceramide synthetic program was attenuated in Angptl4 -/- mice. WT and Angptl4 -/- mice were treated with PBS or dexamethasone for 7 days. ( A to C ) The concentrations of 16 different ceramide species in the liver (A), 5 different free fatty acids (FFAs) in plasma (B), and 4 ceramide species in plasma (C) of these mice were measured. n = 4 mice per group. ( D ) The expression of genes encoding enzymes involved in ceramide production was monitored using quantitative polymerase chain reaction (qPCR). The heat map shows the relative expression compared to that in the WT PBS group. Red shading on the heat map indicates higher expression, and green shading represents lower expression. The changes in fold induction are shown in fig. S1 . n = 16 mice per group. ( E ) Schematic representation of ceramide synthesis pathways. The genes and metabolites that were induced by dexamethasone in WT mice liver are shown in red. The metabolites that were reduced by dexamethasone are shown in green. ( F ) The abundance of Cers5 and Cers6 proteins was monitored using Western blot. The bar graph represents the average intensity of bands normalized to those for Gapdh. Error bars represent SEM. n = 3 to 4 mice per group. * P < 0.05. {TM}

Product Details

PA5-20570

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

2.5 µg/mL

Immunocytochemistry (ICC/IF)

20 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

An 18 amino acid peptide near the amino terminus of the human LASS5.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11152546

Product Specific Information

A suggested positive control is rat brain tissue lysate.

PA5-20570 can be used with blocking peptide PEP-0690.

Target Information

The LASS (longevity assurance homolog) family members represent a subgroup of the homeobox gene family and are highly conserved from yeasts to mammals. Six members of this family of proteins have been characterized (LASS1-6) and all are involved in ceramide synthesis during cell growth regulation and cancer differentiation. LASS5, also called Trh4, is a 392 amino acid endoplasmic reticulum, multi-pass membrane protein. Functioning as a dihydro-ceramide synthase, LASS5 is involved in the production of sphingolipids containing mainly one fatty acid donor (N-linked palmitoyl-ceramide) in a fumonisin B1-independent manner. It uses palmitoyl-CoA as an acyl donor and is involved in the synthesis of C14, C16 and C18-ceramide. LASS5 is the most abundantly expressed and predominant ceramide synthase isoform in lung epithelia. Recent studies show that LASS5 partially correct growth and apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The glucocorticoid-Angptl4-ceramide axis induces insulin resistance through PP2A and PKCζ.

Science signaling

Chen TC,Benjamin DI,Kuo T,Lee RA,Li ML,Mar DJ,Costello DE,Nomura DK,Wang JC

Published figure using Lass5 polyclonal antibody (Product # PA5-20570) in Western Blot

Tue Jul 25 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: Ceramide synthase 5; CerS5; LAG1 homolog, ceramide synthase 5; LAG1 longevity assurance homolog 5; longevity assurance homolog 5; Sphingoid base N-palmitoyltransferase CERS5; Sphingosine N-acyltransferase CERS5; TRAM homolog 4; Translocating chain-associating membrane protein homolog 4

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Gene Aliases: 2310081H14Rik; AU045339; AW544927; CERS5; LASS5; Trh4

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UniProt ID: (Human) B4DV54, (Mouse) Q9D6K9

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Entrez Gene ID: (Human) 91012, (Mouse) 71949, (Rat) 366984

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Function(s)

DNA binding sphingosine N-acyltransferase activity transferase activity

Process(es)

lipid metabolic process sphingolipid metabolic process sphingolipid biosynthetic process ceramide biosynthetic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Lass5 Polyclonal Antibody

Lass5 Antibody (PA5-20570) in ICC/IF

Product Details

PA5-20570

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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