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Invitrogen
Lefty1/Lefty2 Recombinant Rabbit Monoclonal Antibody (ARC2149)
FIGURE: 1 / 3
Lefty1/Lefty2 Antibody (MA5-38044) in IHC (P)
Immunohistochemistry analysis of Lefty1/Lefty2 in paraffin-embedded human colon carcinoma. Samples were incubated with Lefty1/Lefty2 Monoclonal antibody (Product # MA5-38044) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Product Details
MA5-38044
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
ARC2149
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 267-366 of human LEFTY1/2 (O75610)
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2897962
Product Specific Information
Positive Samples: HT-29, BxPC-3, Mouse brain, Rat brain
Immunogen sequence: EMYIDLQGMK WAENWVLEPP GFLAYECVGT CRQPPEALAF KWPFLGPRQC IASETDSLPM IVSIKEGGRT RPQVVSLPNM RVQKCSCASD GALVPRRLQP
Target Information
Left-right determination factor 1 and 2 (Lefty1/2) belong to the TGF-beta superfamily of proteins and are responsible for determination of left-right positioning of visceral organs during embryogenesis. Lefty1 acts as a regulator of Lefty2 and NODAL as the lack of the former resulted in bilateral expression of nodal, lefty2 and Pitx2 (a homeobox gene normally expressed on the left side and blocks the signal for leftness). Lefty2 may play a role in endometrial bleeding.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: endometrial bleeding associated factor; Endometrial bleeding-associated factor; Left-right determination factor 1; Left-right determination factor 2; Left-right determination factor A; Left-right determination factor B; left-right determination, factor A; left-right determination, factor B; Lefty protein; Protein lefty-1; Protein lefty-2; Protein lefty-A; Protein lefty-B; Stimulated by retinoic acid gene 3 protein; TGF-beta-4; Transforming growth factor beta-4
Gene Aliases: 6030463A22Rik; AI450052; AV214969; EBAF; Ebaf2; LEFTA; LEFTB; Lefty; lefty-1; LEFTY1; LEFTY2; LEFTYA; LEFTYB; PSEC0024; RGD1561867; Stra3; TGFB4; UNQ278/PRO317
UniProt ID: (Human) O75610, (Human) O00292, (Mouse) Q64280, (Mouse) Q8BMF7
Entrez Gene ID: (Human) 10637, (Human) 7044, (Rat) 498299, (Mouse) 13590, (Rat) 289316, (Mouse) 320202
negative regulation of transcription from RNA polymerase II promoter
heart morphogenesis
transforming growth factor beta receptor signaling pathway
determination of left/right symmetry
positive regulation of pathway-restricted SMAD protein phosphorylation
cell growth
regulation of apoptotic process
regulation of MAPK cascade
cell development
SMAD protein signal transduction
platelet degranulation
multicellular organism development
negative regulation of cell proliferation
anterior/posterior axis specification
response to retinoic acid
cell migration involved in gastrulation
negative regulation of nodal signaling pathway
negative regulation of nodal receptor complex assembly
growth
biological_process
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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