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|Tested species reactivity||Bacteria|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A whole cell preparation of Legionella pneumophila; ATCC #33152.|
|Storage buffer||PBS, pH 7.2|
|Contains||0.1% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 3 publications below|
PA1-7227 detects Legionella pneumophila.
PA1-7227 has been successfully used in immunofluorescence procedures.
The PA1-7227 immunogen is a whole cell preparation of Legionella pneumophila; ATCC #33152.
Recommended short term storage of less than 6 months at 2-8°C. For extended storage, aliquot and freeze.
The causative agent of what is commonly called Legionnaire's Disease, Legionella pneumophila causes acute pneumonia. The disease can also present itself as an nonpneumonic, influenza-like illness called Pontiac Fever. Legionella is found in aquatic environments where it"e;s growth is supported by amoebae. In human infections Legionella acts as an intracellular parasite and multiplies in alveolar macrophages. The genus Legionella contains 39 recognized species. The immunological diversity of Legionella is due to the existence of a variety of serogroups. Legionella pneumophila has the most with a total of 14 serogroups.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A new method to determine in vivo interactomes reveals binding of the Legionella pneumophila effector PieE to multiple rab GTPases.
PA1-7227 was used in immunocytochemistry to identify PieE as a multidomain Legionella-containing vacuole protein with promiscuous Rab GTPase-binding capacity
|Mousnier A,Schroeder GN,Stoneham CA,So EC,Garnett JA,Yu L,Matthews SJ,Choudhary JS,Hartland EL,Frankel G||mBio (5:null)||2014|
|Not Applicable||Not Cited||
LtpD is a novel Legionella pneumophila effector that binds phosphatidylinositol 3-phosphate and inositol monophosphatase IMPA1.
PA1-7227 was used in immunocytochemistry to study the role of the Legionella pneumophila effector LtpD as a phophoinositide-binding protein involved in bacterial replication
|Harding CR,Mattheis C,Mousnier A,Oates CV,Hartland EL,Frankel G,Schroeder GN||Infection and immunity (81:4261)||2013|
|Not Applicable||Not Cited||
The Dot/Icm effector SdhA is necessary for virulence of Legionella pneumophila in Galleria mellonella and A/J mice.
PA1-7227 was used in immunocytochemistry to study the requirement of Legionella pneumophila SdhA for virulence in Galleria mellonella and mice
|Harding CR,Stoneham CA,Schuelein R,Newton H,Oates CV,Hartland EL,Schroeder GN,Frankel G||Infection and immunity (81:2598)||2013|