|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues K(577) E I Q W K T H E V F D A K S K S A(594) of the extracellular domain of rat OB-R.|
|Purification||Ammonium sulfate precipitation|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:2,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-053 detects leptin (OB-R) from human, mouse, and rat tissues.
PA1-053 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~100 kDa and ~125 kDa protein representing the short and long forms of OB-R from mouse brain extract. This antibody also detects two smaller, unrelated proteins of ~31 kDa and 35 kDa which are unable to be competed with the peptide in mouse liver extract.
The PA1-053 immunogen is a synthetic peptide corresponding to residues K(577) E I Q W K T H E V F D A K S K S A(594) of the extracellular domain of rat OB-R. This sequence is completely conserved in mouse OB-R. PA1-053 immunizing peptide (Cat. # PEP-014) is available for use in neutralization and control experiments.
The leptin (OB-R) receptor is a member of the cytokine receptor superfamily which plays an important role in mammalian body weight homeostasis and energy balance. Ligand-induced activation of the OB-R by the adipose tissue-secreted hormone, leptin, appears to activate downstream signaling events through the Janus kinase (JAK)/signal transducer and activator of transcription (STAT) pathway. Leptin can homo-dimerize OB-R extracellular domains while downstream signaling events are initiated upon homo-dimerization of OB-R intracellular domains. Several isoforms of the OB-R which differ in length and signaling capabilities have been reported. RNA transcripts encoding the OB-R long form have been found in the choroid plexus and in the hypothalamus which has been proposed as a control center for satiety and energy expenditure.
Mutations in either the mouse OB-R (db) or leptin (ob) genes have been shown to result in early-onset obesity. Metabolic abnormalities attributable to these genotypes include hypercorticoidemia, hyperinsulinemia and insulin resistance, hyperglycemia, altered CNS activity, reduced metabolic rate of brown adipose tissue, and a large increase in white adipose tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Dysregulation of leptin signaling in Alzheimer disease: evidence for neuronal leptin resistance.
PA1-053 was used in immunohistochemistry to study the involvement of neuronal leptin resistance in Alzheimer's disease
|Bonda DJ,Stone JG,Torres SL,Siedlak SL,Perry G,Kryscio R,Jicha G,Casadesus G,Smith MA,Zhu X,Lee HG||Journal of neurochemistry (128:162)||2014|
Expression of leptin and its receptor in the murine ovary: possible role in the regulation of oocyte maturation.
PA1-053 was used in immunohistochemistry to investigate the distribution of leptin and its receptor in whole tissue sections of the mouse ovary as well as the corresponding effect on mouse oocyte maturation and changes in oocyte cAMP levels
|Ryan NK,Woodhouse CM,Van der Hoek KH,Gilchrist RB,Armstrong DT,Norman RJ||Biology of reproduction (66:1548)||2002|
Leptin reduces pathology and improves memory in a transgenic mouse model of Alzheimer's disease.
PA1-053 was used in western blot to investigate the effect of leptin treatment on histopathology and memory capability in Alzheimer disease model
|Greco SJ,Bryan KJ,Sarkar S,Zhu X,Smith MA,Ashford JW,Johnston JM,Tezapsidis N,Casadesus G||Journal of Alzheimer's disease : JAD (19:1155)||2010|
ObRa and ObRe are differentially expressed in adipose tissue in aged food-restricted rats: effects on circulating soluble leptin receptor levels.
PA1-053 was used in western blot to investigate the expression of ObRa and ObRe in adipose tissue in aged food-restricted .
|Gallardo N,Arribas C,Villar M,Ros M,Carrascosa JM,Martínez C,Andrés A||Endocrinology (146:4934)||2005|
Estrogen and raloxifene modulate leptin and its receptor in hypothalamus and adipose tissue from ovariectomized rats.
PA1-053 was used in western blot to demonstrate the effect of central and peripheral Ob-Rb expression on estrogen levels.
|Meli R,Pacilio M,Raso GM,Esposito E,Coppola A,Nasti A,Di Carlo C,Nappi C,Di Carlo R||Endocrinology (145:3115)||2004|
Leptin distribution and metabolism in the pregnant rat: transplacental leptin passage increases in late gestation but is reduced by excess glucocorticoids.
PA1-053 was used in western blot to study the transplacental passage of maternal leptin and its status during late pregnancy.
|Smith JT,Waddell BJ||Endocrinology (144:3024)||2003|
Metabolic responses to leptin in obese db/db mice are strain dependent.
PA1-053 was used in western blot to demonstrate that metabolic responses to leptin in obesedb/db mice are strain dependent
|Harris RB,Mitchell TD,Yan X,Simpson JS,Redmann SM||American journal of physiology. Regulatory, integrative and comparative physiology (281:R115)||2001|
A leptin dose-response study in obese (ob/ob) and lean (+/?) mice.
PA1-053 was used in western blot to study the different leptin sensitivity in obese (ob/ob) and lean (+/?) mice
|Harris RB,Zhou J,Redmann SM,Smagin GN,Smith SR,Rodgers E,Zachwieja JJ||Endocrinology (139:8)||1998|
Anti-leptin receptor antibody mimics the stimulation of lipolysis induced by leptin in isolated mouse fat pads.
PA1-053 was used in blocking/activating experiment to study the effect of anti-leptin receptor antibody in isolated mouse fat pads
|Kawaji N,Yoshida A,Motoyashiki T,Morita T,Ueki H||Journal of lipid research (42:1671)||2001|