|Tested species reactivity||Human, Mouse, Non-human primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (311 YRWLQAQKDKMFSQNDTR 328) of Leucine rich alpha 2 glycoprotein 1 (LRG1) protein.|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||2.0-4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
LRG1 is a 347 amino acid containing leucine-rich secreted protein, belonging to leucine-rich repeat (LRR) family of proteins and contains eight LRR (leucine rich) repeats, a galactosamine and four glucosamine oligosaccharides attached and has two intrachain disulfide bonds. LRG1, a regulatory protein, is usually found in plasma and is expressed during granulocyte differentiation. Different functions of LRG1 include protein-protein interaction, signal transduction, cell adhesion and development. LRG1 expression is detected in neutrophil fraction of bone marrow and peripheral blood and in the bone marrow mononuclear cell fraction. LRG1 expression is usually upregulated in early DMSO-induced granulocyte differentiation of human promyelocytic leukemia HL-60 cells and also in GCSF-treated human cells derived from a patient with myeloproliferative disorder. Thus LRG1 acts as a novel marker for early neutrophilic granulocyte differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.