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Invitrogen
Ly-49A/D Monoclonal Antibody (eBio12A8 (12A8)), PE, eBioscience™
FIGURE: 1 / 2
Ly-49A/D Antibody (12-5783-81) in Flow
Staining of C57BL/6 splenocytes with Anti-Mouse NK1.1 APC (Product # 17-5941-82) and 0.06 µg of Rat IgG2a kappa Isotype Control PE (Product # 12-4321-80) (left) or 0.06 µg of Anti-Mouse Ly-49A/D PE (right). Total viable cells were used for analysis.
Product Details
12-5783-81
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
eBio12A8 (12A8)
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_763599
Product Specific Information
Description: The eBio12A8 antibody reacts with Ly-49A and Ly-49D. The Ly-49 family of NK-cell receptors, members of the C-type lectin superfamily, are disulfide-linked type-II transmembrane protein homodimers with extracellular carbohydrate-recognition domains which bind to MHC class I alloantigens. The Ly-49 family members are expressed independently, such that an individual NK or T cell may display more than one class of Ly-49 receptor homodimers. Ly-49D is expressed on subsets of natural killer cells in C57BL/6, C3H/He, and SJL mice, but not DBA/2, AKR, CBA/J, or BALB/c mice. The Ly-49D antigen has not been detected on NK-1.1+ T cells. The Ly-49D receptor has been implicated in mediation of NK cell cytolysis. Ly-49D is an activating receptor while Ly-49A is a inhibitory receptor.
eBio12A8 does not react with BALB/c, DBA/2, AKR, or CBA/J Ly-D49D.
Applications Reported: This eBio12A8 (12A8) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBio12A8 (12A8) antibody has been tested by flow cytometric analysis of mouse spleen cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The Ly-49 family of NK-cell receptors, members of the C-type lectin superfamily, are disulfide-linked type-II transmembrane protein homodimers with extracellular carbohydrate-recognition domains which bind to MHC class I alloantigens. The Ly-49 family members are expressed independently, such that an individual NK or T cell may display more than one class of Ly-49 receptor homodimers. Ly-49D is expressed on subsets of natural killer cells in C57BL/6, C3H/He, and SJL mice, but not DBA/2, AKR, CBA/J, or BALB/c mice. The Ly-49D antigen has not been detected on NK-1.1+ T cells. The Ly-49D receptor has been implicated in mediation of NK cell cytolysis. Ly-49D is an activating receptor while Ly-49A is a inhibitory receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: class I MHC receptor KLRA22; Killer cell lectin-like receptor 4; killer cell lectin-like receptor subfamily A member 32; killer cell lectin-like receptor subfamily A member 33; ly-49a; Ly-49d; lymphocyte antigen 49a; Lymphocyte antigen 49d; natural killer cell receptor Ly49A; novel killer cell lectin-like receptor subfamily A protein; t lymphocyte antigen A1; T-cell surface glycoprotein Ly-49D; T-cell surface glycoprotein YE1/48
Gene Aliases: A1; CH29-493D4.3; Chok; Klra22; Klra32; Klra33; Klra4; Ly-49d; Ly49-d; Ly49a; Ly49d; Ly49o<129>; ly49r<129>; Ly49v
UniProt ID: (Mouse) Q60651
Entrez Gene ID: (Mouse) 16627, (Mouse) 16635
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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