Invitrogen

Ly-6A/E Monoclonal Antibody (D7)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

16 References

View all (24) Ly-6A/E (Sca-1) antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

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Ly-6A/E Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Lymphocyte antigen 6A-2/6E-1 was performed using 70% confluent log phase C2C12 cells differentiated to myotubes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Ly-6A/E Monoclonal Antibody (D7) (Product # MA1-70082) at 5 ug/ml in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Product # A11006), (1:1500), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membraneous localization. Panel e represents undifferentiated cells having low signal . Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Ly-6A/E Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of bone marrow staining using a Ly 6A/E monoclonal antibody (Product # MA1-70082) at a 0.5 µg/10^6 cells dilution. The isotypic control was a biotin rat IgG2a

Ly-6A/E Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using Anti-Ly-6A/E Monoclonal Antibody (D7) (Product # MA1-70082), shows increased expression of Sca1 only in C2C12 differentiated to myotube cells as compared to undifferentiated cells. {RE}

Product Details

MA1-70082

Applications	Tested Dilution	Publications
Western Blot (WB)	Assay-dependent	View 2 publications 2 publications
Immunocytochemistry (ICC/IF)	5 µg/mL	-
Flow Cytometry (Flow)	1 µg/1 million cells	View 14 publications 14 publications
Immunoprecipitation (IP)	Assay-dependent	-

Product Specifications
Species Reactivity	Mouse
Published species	Bovine, Mouse
Host/Isotype	Rat / IgG2a
Class	Monoclonal
Type	Antibody
Clone	D7
Immunogen	IL-2-dependent mouse T-cell line CTL-L.
Conjugate	Unconjugated Biotin PE
Form	Liquid
Concentration	0.5 mg/mL
Purification	Protein G
Storage buffer	PBS
Contains	0.09% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_1077516

Product Specific Information

MA1-70082 detects Ly 6A/E in mouse samples.

MA1-70082 has been successfully used in Western blot, flow cytometry and immunoprecipitation procedures.

The MA1-70082 immunogen is IL-2-dependent mouse T-cell line CTL-L.

Target Information

Stem Cell Antigen-1 (Sca-1) is a member of the lymphocyte antigen 6 (Ly-6) family. It is expressed on multipotent hematopoietic stem cells (HSC) and is used as a marker of HSC in mice. Sca-1 is used in combination with lineage depletion antibodies to identify murine HSC. Sca-1 can be found in fetal liver, adult bone marrow, and adult peripheral blood and spleen. Sca-1 may be involved in the regulation of B and T cell activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Osteopontin attenuates aging-associated phenotypes of hematopoietic stem cells.

The EMBO journal

Guidi N,Sacma M,Ständker L,Soller K,Marka G,Eiwen K,Weiss JM,Kirchhoff F,Weil T,Cancelas JA,Florian MC,Geiger H

MA170082 was used in flow cytometry to examine the contribution of stroma-derived osteopontin for hematopoietic stem cell aging

Mon Apr 03 00:00:00 EDT 2017

A population of hematopoietic stem cells derives from GATA4-expressing progenitors located in the placenta and lateral mesoderm of mice.

Haematologica

Cañete A,Carmona R,Ariza L,Sánchez MJ,Rojas A,Muñoz-Chápuli R

MA170082 was used in flow cytometry to utilize a G2-Gata4Cre;R26REYFP mouse line to track the developmental fate of the G2-Gata4 cell lineage

Sat Apr 01 00:00:00 EDT 2017

Combined blockade of the IL-13 and IL-33 pathways leads to a greater inhibition of type 2 inflammation over inhibition of either pathway alone.

The Journal of allergy and clinical immunology

Ramirez-Carrozzi V,Sambandam A,Zhou M,Yan D,Kang J,Wu X,Suto E,Baca M,Austin C,Xu M,Lee W,Pappu R

MA170082 was used in flow cytometry to study the effect of IL-13 and IL-33 pathways in dysregulated type 2 inflammation.

Wed Feb 01 00:00:00 EST 2017

Inhibition of Stat3 signaling ameliorates atrophy of the soleus muscles in mice lacking the vitamin D receptor.

Skeletal muscle

Gopinath SD

MA170082 was used in flow cytometry to assess the roles of signal transducer and activator of transcription 3 signaling and vitamin D in skeletal muscles

Wed Jan 25 00:00:00 EST 2017

GRK6 regulates ROS response and maintains hematopoietic stem cell self-renewal.

Cell death & disease

Le Q,Yao W,Chen Y,Yan B,Liu C,Yuan M,Zhou Y,Ma L

MA170082 was used in flow cytometry to use knockout mice to determine if GRK6 contributes to hematopoiesis

Thu Nov 24 00:00:00 EST 2016

Identification of blood vascular endothelial stem cells by the expression of protein C receptor.

Cell research

Yu QC,Song W,Wang D,Zeng YA

MA170082 was used in flow cytometry to determine the origin of pericytes in adult angiogenesis

Sat Oct 01 00:00:00 EDT 2016

Carbonic anhydrase enzymes regulate mast cell-mediated inflammation.

The Journal of experimental medicine

Henry EK,Sy CB,Inclan-Rico JM,Espinosa V,Ghanny SS,Dwyer DF,Soteropoulos P,Rivera A,Siracusa MC

MA170082 was used in flow cytometry to find a role for Car enzymes in regulating mast cell lineage commitment

Mon Aug 22 00:00:00 EDT 2016

Group 3 innate lymphoid cells continuously require the transcription factor GATA-3 after commitment.

Nature immunology

Zhong C,Cui K,Wilhelm C,Hu G,Mao K,Belkaid Y,Zhao K,Zhu J

MA170082 was used in flow cytometry to explore the contributions of GATA3 to the group 3 innate lymphoid cells

Mon Feb 01 00:00:00 EST 2016

Haematopoietic ESL-1 enables stem cell proliferation in the bone marrow by limiting TGFβ availability.

Nature communications

Leiva M,Quintana JA,Ligos JM,Hidalgo A

MA1-70082 was used in flow cytometry to examine the effect of E-selectin ligand 1 on hematopoietic cells

Fri Jan 08 00:00:00 EST 2016

Adipocyte progenitor cells initiate monocyte chemoattractant protein-1-mediated macrophage accumulation in visceral adipose tissue.

Molecular metabolism

Kaplan JL,Marshall MA,C McSkimming C,Harmon DB,Garmey JC,Oldham SN,Hallowell P,McNamara CA

MA1-70082 was used in flow cytometry to identify the source of obesity-induced MCP-1 and identify molecular regulators mediating MCP-1 production

Sun Nov 01 00:00:00 EDT 2015

Metformin ameliorates ionizing irradiation-induced long-term hematopoietic stem cell injury in mice.

Free radical biology & medicine

Xu G,Wu H,Zhang J,Li D,Wang Y,Wang Y,Zhang H,Lu L,Li C,Huang S,Xing Y,Zhou D,Meng A

MA1-70082 was used in flow cytometry to test if metformin ameliorates ionizing radiation-induced long-term bone marrow injury in a total-body irradiation mouse model

Thu Oct 01 00:00:00 EDT 2015

BCL11A is a triple-negative breast cancer gene with critical functions in stem and progenitor cells.

Nature communications

Khaled WT,Choon Lee S,Stingl J,Chen X,Raza Ali H,Rueda OM,Hadi F,Wang J,Yu Y,Chin SF,Stratton M,Futreal A,Jenkins NA,Aparicio S,Copeland NG,Watson CJ,Caldas C,Liu P

MA170082 was used in flow cytometry to investigate the impact of BCL11A expression on breast cancer development

Fri Jan 09 00:00:00 EST 2015

The transcription factor T-bet regulates intestinal inflammation mediated by interleukin-7 receptor+ innate lymphoid cells.

Immunity

Powell N,Walker AW,Stolarczyk E,Canavan JB,Gökmen MR,Marks E,Jackson I,Hashim A,Curtis MA,Jenner RG,Howard JK,Parkhill J,MacDonald TT,Lord GM

MA1-70082 was used in flow cytometry to examine IL-7R signaling in Tbx21 and Rag2 knockout mice

Fri Oct 19 00:00:00 EDT 2012

Dependence of RGS9-1 membrane attachment on its C-terminal tail.

The Journal of biological chemistry

He W,Melia TJ,Cowan CW,Wensel TG

MA1-70082 was used in western blot to investigate membrane attachment of RGS9-1

Fri Dec 28 00:00:00 EST 2001

Expression of Ly-6A/E alloantigens in thymocyte and T-lymphocyte subsets: variability related to the Ly-6a and Ly-6b haplotypes.

Immunogenetics

Codias EK,Cray C,Baler RD,Levy RB,Malek TR

MA1-70082 was used in flow cytometry to elucidate the cellular basis for differential expression of the Ly-6A/E alloantigen on T cells

Tue Mar 14 00:00:00 EST 1989

Ly-6A.2 and Ly-6E.1 molecules are antithetical and identical to MALA-1.

Immunogenetics

Palfree RG,Dumont FJ,Hammerling U

MA1-70082 was used in western blot to discuss detection of Ly-6 locus-controlled antigen

Mon May 19 00:00:00 EDT 1986

Bioinformatics

Protein Aliases: locus A/E; Locus AE; Ly-6A.2/Ly-6E.1; Ly-6E; ly6a-e; Ly6A/E; lymphocyte antigen 6 complex; Lymphocyte antigen 6A-2/6E-1; Lymphocyte antigen 6E; SCA-1; Stem cell antigen 1; Stem cell antigen 2; T-cell-activating protein; TAP; Thymic shared antigen 1; TSA-1

Gene Aliases: 9804; Ly-6A.2; Ly-6A/E; Ly-6E.1; Ly6; Ly67; Ly6a; Ly6e; RIG-E; Sca-1; Sca-2; Sca1; TAP; TSA-1; Tsa1

UniProt ID: (Mouse) P05533, (Mouse) Q64253

Entrez Gene ID: (Mouse) 110454, (Mouse) 17069

Function(s)

in utero embryonic development adrenal gland development organ growth norepinephrine metabolic process epinephrine secretion ventricular cardiac muscle tissue morphogenesis