Invitrogen

Ly-6A/E (Sca-1) Monoclonal Antibody (D7), PE-eFluor™ 610, eBioscience™

Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 99

Ly-6A/E (Sca-1) Antibody (61-5981-82) in Flow

Staining of C57Bl/6 bone marrow cells with Mouse Hematopoietic Lineage Cocktail eFluor® 450 (Product # 88-7772-72), Anti-Mouse CD117 (c-Kit) APC (Product # 17-1171-82), and 0.25 µg of Rat IgG2a K Isotype Control PE-eFluor® 610 (Product # 61-4321-82) (left) or 0.25 µg of Anti-Mouse Ly-6A/E (Sca-1) PE-eFluor® 610 (right). Lineage negative/low viable cells, as determined by Fixable Viability Dye eFluor® 506 (Product # 65-0866-14), were used for analysis.

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Product Details

61-5981-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 77 publications 77 publications

Product Specifications

Species Reactivity

Mouse

Published species

Fish, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE-eFluor™ 610, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

Conjugate

PE-eFluor™ 610

Excitation/Emission Max

565/606 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

RRID

AB_2574648

Product Specific Information

Description: The D7 monoclonal antibody reacts with mouse Sca-1, an 18 kDa member of the Ly-6 family of GPI-linked surface proteins. D7 reacts with both Ly-6E.1 and Ly-6A.2 molecules expressed by mouse hematopoietic stem cells, myeloid population and peripheral T and B cells. Non-activated splenocytes from Ly-6.2 strains including C57BL, SJL, 129, AKR, and others demonstrate higher frequency of D7-positive cells compared to the Ly-6.1 strains such as BALB/c, C3H, NZB and others. Sca-1 is expressed at high levels upon activation regardless of the Ly-6 haplotype. The Ly-6 family is involved in regulation and function of T cell activation. Sca-1 is a major phenotypic marker for mouse hematopoietic progenitor/stem cell subset.

Applications Reported: This D7 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This D7 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Stem Cell Antigen-1 (Sca-1) is a member of the lymphocyte antigen 6 (Ly-6) family. It is expressed on multipotent hematopoietic stem cells (HSC) and is used as a marker of HSC in mice. Sca-1 is used in combination with lineage depletion antibodies to identify murine HSC. Sca-1 can be found in fetal liver, adult bone marrow, and adult peripheral blood and spleen. Sca-1 may be involved in the regulation of B and T cell activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

The extracellular matrix controls stem cell specification and crypt morphology in the developing and adult mouse gut.

Biology open

Ramadan R,Wouters VM,van Neerven SM,de Groot NE,Garcia TM,Muncan V,Franklin OD,Battle M,Carlson KS,Leach J,Sansom OJ,Boulard O,Chamaillard M,Vermeulen L,Medema JP,Huels DJ

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Thu Dec 15 00:00:00 EST 2022

Cardiac Mesenchymal Stem Cells Promote Fibrosis and Remodeling in Heart Failure: Role of PDGF Signaling.

JACC. Basic to translational science

Hamid T,Xu Y,Ismahil MA,Rokosh G,Jinno M,Zhou G,Wang Q,Prabhu SD

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Sun May 01 00:00:00 EDT 2022

Hematopoietic-Mesenchymal Signals Regulate the Properties of Mesenchymal Stem Cells.

International journal of molecular sciences

Kanazawa S,Okada H,Riu D,Mabuchi Y,Akazawa C,Iwata J,Hoshi K,Hikita A

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Tue Jul 26 00:00:00 EDT 2022

Dynamic and adaptive cancer stem cell population admixture in colorectal neoplasia.

Cell stem cell

Vasquez EG,Nasreddin N,Valbuena GN,Mulholland EJ,Belnoue-Davis HL,Eggington HR,Schenck RO,Wouters VM,Wirapati P,Gilroy K,Lannagan TRM,Flanagan DJ,Najumudeen AK,Omwenga S,McCorry AMB,Easton A,Koelzer VH,East JE,Morton D,Trusolino L,Maughan T,Campbell AD,Loughrey MB,Dunne PD,Tsantoulis P,Huels DJ,Tejpar S,Sansom OJ,Leedham SJ

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Thu Aug 04 00:00:00 EDT 2022

Nociceptive nerves regulate haematopoietic stem cell mobilization.

Nature

Gao X,Zhang D,Xu C,Li H,Caron KM,Frenette PS

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Photoreceptor protection by mesenchymal stem cell transplantation identifies exosomal MiR-21 as a therapeutic for retinal degeneration.

Cell death and differentiation

Deng CL,Hu CB,Ling ST,Zhao N,Bao LH,Zhou F,Xiong YC,Chen T,Sui BD,Yu XR,Hu CH

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Mon Mar 01 00:00:00 EST 2021

Loss of p53 in mesenchymal stem cells promotes alteration of bone remodeling through negative regulation of osteoprotegerin.

Cell death and differentiation

Velletri T,Huang Y,Wang Y,Li Q,Hu M,Xie N,Yang Q,Chen X,Chen Q,Shou P,Gan Y,Candi E,Annicchiarico-Petruzzelli M,Agostini M,Yang H,Melino G,Shi Y,Wang Y

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

RANKL from bone marrow adipose lineage cells promotes osteoclast formation and bone loss.

EMBO reports

Hu Y,Li X,Zhi X,Cong W,Huang B,Chen H,Wang Y,Li Y,Wang L,Fang C,Guo J,Liu Y,Cui J,Cao L,Weng W,Zhou Q,Wang S,Chen X,Su J

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Mon Jul 05 00:00:00 EDT 2021

Effects of signaling pathway inhibitors on hematopoietic stem cells.

Molecular medicine reports

Jiang Y,Xu Z,Ma N,Yin L,Hao C,Li J

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

BMC musculoskeletal disorders

Xu W,Ni C,Wang Y,Zheng G,Zhang J,Xu Y

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Tue Apr 27 00:00:00 EDT 2021

Activation of skeletal muscle-resident glial cells upon nerve injury.

JCI insight

Proietti D,Giordani L,De Bardi M,D'Ercole C,Lozanoska-Ochser B,Amadio S,Volonté C,Marinelli S,Muchir A,Bouché M,Borsellino G,Sacco A,Puri PL,Madaro L

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Thu Apr 08 00:00:00 EDT 2021

Therapeutic Potential of Mesenchymal Stem Cells in a Pre-Clinical Model of Diabetic Kidney Disease and Obesity.

International journal of molecular sciences

Sávio-Silva C,Soinski-Sousa PE,Simplício-Filho A,Bastos RMC,Beyerstedt S,Rangel ÉB

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Thu Feb 04 00:00:00 EST 2021

MAEA is an E3 ubiquitin ligase promoting autophagy and maintenance of haematopoietic stem cells.

Nature communications

Wei Q,Pinho S,Dong S,Pierce H,Li H,Nakahara F,Xu J,Xu C,Boulais PE,Zhang D,Maryanovich M,Cuervo AM,Frenette PS

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Tue May 04 00:00:00 EDT 2021

The IRE1/XBP1 signaling axis promotes skeletal muscle regeneration through a cell non-autonomous mechanism.

eLife

Roy A,Tomaz da Silva M,Bhat R,Bohnert KR,Iwawaki T,Kumar A

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Tue Nov 23 00:00:00 EST 2021

Troy/Tnfrsf19 marks epidermal cells that govern interfollicular epidermal renewal and cornification.

Stem cell reports

Kretzschmar K,Boonekamp KE,Bleijs M,Asra P,Koomen M,Chuva de Sousa Lopes SM,Giovannone B,Clevers H

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Tue Sep 14 00:00:00 EDT 2021

Conditionally pathogenic genetic variants of a hematopoietic disease-suppressing enhancer.

Science advances

Soukup AA,Matson DR,Liu P,Johnson KD,Bresnick EH

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Fri Dec 10 00:00:00 EST 2021

Multipotency of mouse trophoblast stem cells.

Stem cell research & therapy

Hou M,Han J,Li G,Kwon MY,Jiang J,Emani S,Taglauer ES,Park JA,Choi EB,Vodnala M,Fong YW,Emani SM,Rosas IO,Perrella MA,Liu X

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Thu Feb 13 00:00:00 EST 2020

Diamond Blackfan anemia is mediated by hyperactive Nemo-like kinase.

Nature communications

Wilkes MC,Siva K,Chen J,Varetti G,Youn MY,Chae H,Ek F,Olsson R,Lundbäck T,Dever DP,Nishimura T,Narla A,Glader B,Nakauchi H,Porteus MH,Repellin CE,Gazda HT,Lin S,Serrano M,Flygare J,Sakamoto KM

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Fri Jul 03 00:00:00 EDT 2020

XIAOPI formula inhibits the pre-metastatic niche formation in breast cancer via suppressing TAMs/CXCL1 signaling.

Cell communication and signaling : CCS

Zheng Y,Wang N,Wang S,Yang B,Situ H,Zhong L,Lin Y,Wang Z

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Thu Mar 26 00:00:00 EDT 2020

Use of Busulfan to Condition Mice for Bone Marrow Transplantation.

STAR protocols

Montecino-Rodriguez E,Dorshkind K

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Fri Dec 18 00:00:00 EST 2020

Phosphatase SHP1 impedes mesenchymal stromal cell immunosuppressive capacity modulated by JAK1/STAT3 and P38 signals.

Cell & bioscience

Jiang M,Ye J,Wang X,Li N,Wang Y,Shi Y

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Thu Apr 14 00:00:00 EDT 2022

Interaction of Deubiquitinase 2A-DUB/MYSM1 with DNA Repair and Replication Factors.

International journal of molecular sciences

Kroeger C,Roesler R,Wiese S,Hainzl A,Gatzka MV

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Tue May 26 00:00:00 EDT 2020

A novel function of R-spondin1 in regulating estrogen receptor expression independent of Wnt/β-catenin signaling.

eLife

Geng A,Wu T,Cai C,Song W,Wang J,Yu QC,Zeng YA

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Tue Aug 04 00:00:00 EDT 2020

Apoptotic bodies derived from mesenchymal stem cells promote cutaneous wound healing via regulating the functions of macrophages.

Stem cell research & therapy

Liu J,Qiu X,Lv Y,Zheng C,Dong Y,Dou G,Zhu B,Liu A,Wang W,Zhou J,Liu S,Liu S,Gao B,Jin Y

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Fri Nov 27 00:00:00 EST 2020

miR-142 induces accumulation of reactive oxygen species (ROS) by inhibiting pexophagy in aged bone marrow mesenchymal stem cells.

Scientific reports

Houri K,Mori T,Onodera Y,Tsujimoto T,Takehara T,Nakao S,Teramura T,Fukuda K

61-5981-82 was used in Flow Cytometry to support a novel molecular mechanism relating aging-associated ROS generation and organelle degradation in BMMSCs, and suggest a potential therapeutic target for aging-associated disorders that are accompanied by stem cell degeneration.

Fri Feb 28 00:00:00 EST 2020

Long-term expansion and differentiation of adult murine epidermal stem cells in 3D organoid cultures.

Proceedings of the National Academy of Sciences of the United States of America

Boonekamp KE,Kretzschmar K,Wiener DJ,Asra P,Derakhshan S,Puschhof J,López-Iglesias C,Peters PJ,Basak O,Clevers H

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Tue Jul 16 00:00:00 EDT 2019

PDGFRA defines the mesenchymal stem cell Kaposi's sarcoma progenitors by enabling KSHV oncogenesis in an angiogenic environment.

PLoS pathogens

Naipauer J,Rosario S,Gupta S,Premer C,Méndez-Solís O,Schlesinger M,Ponzinibbio V,Jain V,Gay L,Renne R,Chan HL,Morey L,Salyakina D,Abba M,Williams S,Hare JM,Goldschmidt-Clermont PJ,Mesri EA

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Sun Dec 01 00:00:00 EST 2019

Transforming Growth Factor β-Activated Kinase 1 Regulates Mesenchymal Stem Cell Proliferation Through Stabilization of Yap1/Taz Proteins.

Stem cells (Dayton, Ohio)

Onodera Y,Teramura T,Takehara T,Fukuda K

61-5981 was used in Flow cytometry/Cell sorting to reveal a novel pathway controlling bone marrow-derived mesenchymal stem cells (BMMSC) proliferation and suggests a useful method to improve the therapeutic effect of BMMSC transplantation.

Sun Dec 01 00:00:00 EST 2019

Transforming Growth Factor β-Activated Kinase 1 Regulates Mesenchymal Stem Cell Proliferation Through Stabilization of Yap1/Taz Proteins.

Stem cells (Dayton, Ohio)

Onodera Y,Teramura T,Takehara T,Fukuda K

Sun Dec 01 00:00:00 EST 2019

Macrophages fine tune satellite cell fate in dystrophic skeletal muscle of mdx mice.

PLoS genetics

Madaro L,Torcinaro A,De Bardi M,Contino FF,Pelizzola M,Diaferia GR,Imeneo G,Bouchè M,Puri PL,De Santa F

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2019

TGFβ-induced degradation of TRAF3 in mesenchymal progenitor cells causes age-related osteoporosis.

Nature communications

Li J,Ayoub A,Xiu Y,Yin X,Sanders JO,Mesfin A,Xing L,Yao Z,Boyce BF

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Wed Jun 26 00:00:00 EDT 2019

Excessive exosome release is the pathogenic pathway linking a lysosomal deficiency to generalized fibrosis.

Science advances

van de Vlekkert D,Demmers J,Nguyen XX,Campos Y,Machado E,Annunziata I,Hu H,Gomero E,Qiu X,Bongiovanni A,Feghali-Bostwick CA,d'Azzo A

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Mon Jul 01 00:00:00 EDT 2019

The microRNAs miR-204 and miR-211 maintain joint homeostasis and protect against osteoarthritis progression.

Nature communications

Huang J,Zhao L,Fan Y,Liao L,Ma PX,Xiao G,Chen D

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Fri Jun 28 00:00:00 EDT 2019

Procr-expressing progenitor cells are responsible for murine ovulatory rupture repair of ovarian surface epithelium.

Nature communications

Wang J,Wang D,Chu K,Li W,Zeng YA

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Thu Oct 31 00:00:00 EDT 2019

PDGF is a potent initiator of bone formation in a tissue engineered model of pathological ossification.

Journal of tissue engineering and regenerative medicine

Davies OG,Grover LM,Lewis MP,Liu Y

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Mon Jan 01 00:00:00 EST 2018

The H3K4 methyltransferase Setd1b is essential for hematopoietic stem and progenitor cell homeostasis in mice.

eLife

Schmidt K,Zhang Q,Tasdogan A,Petzold A,Dahl A,Arneth BM,Slany R,Fehling HJ,Kranz A,Stewart AF,Anastassiadis K

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Tue Jun 19 00:00:00 EDT 2018

Tracking of Adipose-Derived Mesenchymal Stromal/Stem Cells in a Model of Cisplatin-Induced Acute Kidney Injury: Comparison of Bioluminescence Imaging versus qRT-PCR.

International journal of molecular sciences

Schubert R,Sann J,Frueh JT,Ullrich E,Geiger H,Baer PC

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Wed Aug 29 00:00:00 EDT 2018

Differential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus.

eLife

Moretti FA,Klapproth S,Ruppert R,Margraf A,Weber J,Pick R,Scheiermann C,Sperandio M,Fässler R,Moser M

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Thu Sep 06 00:00:00 EDT 2018

HMGB2 is a novel adipogenic factor that regulates ectopic fat infiltration in skeletal muscles.

Scientific reports

Lee D,Taniguchi N,Sato K,Choijookhuu N,Hishikawa Y,Kataoka H,Morinaga H,Lotz M,Chosa E

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Mon Jun 25 00:00:00 EDT 2018

Stem cell factor is selectively secreted by arterial endothelial cells in bone marrow.

Nature communications

Xu C,Gao X,Wei Q,Nakahara F,Zimmerman SE,Mar J,Frenette PS

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Fri Jun 22 00:00:00 EDT 2018

RhoA, Rac1, and Cdc42 differentially regulate αSMA and collagen I expression in mesenchymal stem cells.

The Journal of biological chemistry

Ge J,Burnier L,Adamopoulou M,Kwa MQ,Schaks M,Rottner K,Brakebusch C

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Fri Jun 15 00:00:00 EDT 2018

Bone marrow-derived immature myeloid cells are a main source of circulating suPAR contributing to proteinuric kidney disease.

Nature medicine

Hahm E,Wei C,Fernandez I,Li J,Tardi NJ,Tracy M,Wadhwani S,Cao Y,Peev V,Zloza A,Lusciks J,Hayek SS,O'Connor C,Bitzer M,Gupta V,Sever S,Sykes DB,Scadden DT,Reiser J

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Sun Jan 01 00:00:00 EST 2017

FOXP1 controls mesenchymal stem cell commitment and senescence during skeletal aging.

The Journal of clinical investigation

Li H,Liu P,Xu S,Li Y,Dekker JD,Li B,Fan Y,Zhang Z,Hong Y,Yang G,Tang T,Ren Y,Tucker HO,Yao Z,Guo X

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Mon Apr 03 00:00:00 EDT 2017

GATA4-dependent organ-specific endothelial differentiation controls liver development and embryonic hematopoiesis.

The Journal of clinical investigation

Géraud C,Koch PS,Zierow J,Klapproth K,Busch K,Olsavszky V,Leibing T,Demory A,Ulbrich F,Diett M,Singh S,Sticht C,Breitkopf-Heinlein K,Richter K,Karppinen SM,Pihlajaniemi T,Arnold B,Rodewald HR,Augustin HG,Schledzewski K,Goerdt S

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2017

Induced p53 loss in mouse luminal cells causes clonal expansion and development of mammary tumours.

Nature communications

Tao L,Xiang D,Xie Y,Bronson RT,Li Z

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Mon Feb 13 00:00:00 EST 2017

Mutant U2AF1-expressing cells are sensitive to pharmacological modulation of the spliceosome.

Nature communications

Shirai CL,White BS,Tripathi M,Tapia R,Ley JN,Ndonwi M,Kim S,Shao J,Carver A,Saez B,Fulton RS,Fronick C,O'Laughlin M,Lagisetti C,Webb TR,Graubert TA,Walter MJ

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Mon Jan 09 00:00:00 EST 2017

Microgrooved-surface topography enhances cellular division and proliferation of mouse bone marrow-derived mesenchymal stem cells.

PloS one

Chaudhary JK,Rath PC

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Thu Oct 19 00:00:00 EDT 2017

Zoledronate suppressed angiogenesis and osteogenesis by inhibiting osteoclasts formation and secretion of PDGF-BB.

PloS one

Gao SY,Zheng GS,Wang L,Liang YJ,Zhang SE,Lao XM,Li K,Liao GQ

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Mon Sep 18 00:00:00 EDT 2017

The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity.

Cell

Damgaard RB,Walker JA,Marco-Casanova P,Morgan NV,Titheradge HL,Elliott PR,McHale D,Maher ER,McKenzie ANJ,Komander D

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Thu Aug 25 00:00:00 EDT 2016

Hedgehog-driven myogenic tumors recapitulate skeletal muscle cellular heterogeneity.

Experimental cell research

Hettmer S,Lin MM,Tchessalova D,Tortorici SJ,Castiglioni A,Desai T,Mao J,McMahon AP,Wagers AJ

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2016

Identification of an epidermal keratinocyte AMPA glutamate receptor involved in dermatopathies associated with sensory abnormalities.

Pain reports

Cabañero D,Irie T,Celorrio M,Trousdale C,Owens DM,Virley D,Albrecht PJ,Caterina MJ,Rice FL,Morón JA

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Thu Sep 01 00:00:00 EDT 2016

KLF4-dependent phenotypic modulation of smooth muscle cells has a key role in atherosclerotic plaque pathogenesis.

Nature medicine

Shankman LS,Gomez D,Cherepanova OA,Salmon M,Alencar GF,Haskins RM,Swiatlowska P,Newman AA,Greene ES,Straub AC,Isakson B,Randolph GJ,Owens GK

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2015

Rapid depot-specific activation of adipocyte precursor cells at the onset of obesity.

Nature cell biology

Jeffery E,Church CD,Holtrup B,Colman L,Rodeheffer MS

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2015

Cis-regulatory mechanisms governing stem and progenitor cell transitions.

Science advances

Johnson KD,Kong G,Gao X,Chang YI,Hewitt KJ,Sanalkumar R,Prathibha R,Ranheim EA,Dewey CN,Zhang J,Bresnick EH

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2015

Cellular and molecular characterization of human cardiac stem cells reveals key features essential for their function and safety.

Stem cells and development

Vahdat S,Mousavi SA,Omrani G,Gholampour M,Sotoodehnejadnematalahi F,Ghazizadeh Z,Gharechahi J,Baharvand H,Salekdeh GH,Aghdami N

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Mon Jun 15 00:00:00 EDT 2015

Multi-Drug Resistance ABC Transporter Inhibition Enhances Murine Ventral Prostate Stem/Progenitor Cell Differentiation.

Stem cells and development

Samant MD,Jackson CM,Felix CL,Jones AJ,Goodrich DW,Foster BA,Huss WJ

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Fri May 15 00:00:00 EDT 2015

Limited miR-17-92 overexpression drives hematologic malignancies.

Leukemia research

Danielson LS,Reavie L,Coussens M,Davalos V,Castillo-Martin M,Guijarro MV,Coffre M,Cordon-Cardo C,Aifantis I,Ibrahim S,Liu C,Koralov SB,Hernando E

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Sun Mar 01 00:00:00 EST 2015

Cardiogenic genes expressed in cardiac fibroblasts contribute to heart development and repair.

Circulation research

Furtado MB,Costa MW,Pranoto EA,Salimova E,Pinto AR,Lam NT,Park A,Snider P,Chandran A,Harvey RP,Boyd R,Conway SJ,Pearson J,Kaye DM,Rosenthal NA

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Fri Apr 25 00:00:00 EDT 2014

WASH is required for the differentiation commitment of hematopoietic stem cells in a c-Myc-dependent manner.

The Journal of experimental medicine

Xia P,Wang S,Huang G,Zhu P,Li M,Ye B,Du Y,Fan Z

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Mon Sep 22 00:00:00 EDT 2014

Overexpressing sonic hedgehog peptide restores periosteal bone formation in a murine bone allograft transplantation model.

Molecular therapy : the journal of the American Society of Gene Therapy

Huang C,Tang M,Yehling E,Zhang X

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Sat Feb 01 00:00:00 EST 2014

Cell-autonomous heparanase modulates self-renewal and migration in bone marrow-derived mesenchymal stem cells.

Journal of biomedical science

Cheng CC,Lee YH,Lin SP,Huangfu WC,Liu IH

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Thu Mar 13 00:00:00 EDT 2014

Recruitment of bone marrow-derived cells to periodontal tissue defects.

Frontiers in cell and developmental biology

Kimura Y,Komaki M,Iwasaki K,Sata M,Izumi Y,Morita I

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Mon Nov 03 00:00:00 EST 2014

Evidence for Transfer of Membranes from Mesenchymal Stem Cells to HL-1 Cardiac Cells.

Stem cells international

Boomsma RA,Geenen DL

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Wed Oct 08 00:00:00 EDT 2014

Amniotic fluid stem cells prevent follicle atresia and rescue fertility of mice with premature ovarian failure induced by chemotherapy.

PloS one

Xiao GY,Liu IH,Cheng CC,Chang CC,Lee YH,Cheng WT,Wu SC

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Thu May 28 00:00:00 EDT 2015

Validation of the effects of TGF-β1 on tumor recurrence and prognosis through tumor retrieval and cell mechanical properties.

Cancer cell international

Wu TH,Chou YW,Chiu PH,Tang MJ,Hu CW,Yeh ML

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Mon Mar 03 00:00:00 EST 2014

A mouse model for inducible overexpression of Prdm14 results in rapid-onset and highly penetrant T-cell acute lymphoblastic leukemia (T-ALL).

Disease models & mechanisms

Carofino BL,Ayanga B,Justice MJ

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2013

Lunatic fringe deficiency cooperates with the Met/Caveolin gene amplicon to induce basal-like breast cancer.

Cancer cell

Xu K,Usary J,Kousis PC,Prat A,Wang DY,Adams JR,Wang W,Loch AJ,Deng T,Zhao W,Cardiff RD,Yoon K,Gaiano N,Ling V,Beyene J,Zacksenhaus E,Gridley T,Leong WL,Guidos CJ,Perou CM,Egan SE

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Tue May 15 00:00:00 EDT 2012

Concomitant inactivation of Rb and E2f8 in hematopoietic stem cells synergizes to induce severe anemia.

Blood

Hu T,Ghazaryan S,Sy C,Wiedmeyer C,Chang V,Wu L

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Thu May 10 00:00:00 EDT 2012

Cis-element mutated in GATA2-dependent immunodeficiency governs hematopoiesis and vascular integrity.

The Journal of clinical investigation

Johnson KD,Hsu AP,Ryu MJ,Wang J,Gao X,Boyer ME,Liu Y,Lee Y,Calvo KR,Keles S,Zhang J,Holland SM,Bresnick EH

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Mon Oct 01 00:00:00 EDT 2012

CTC1 deletion results in defective telomere replication, leading to catastrophic telomere loss and stem cell exhaustion.

The EMBO journal

Gu P,Min JN,Wang Y,Huang C,Peng T,Chai W,Chang S

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Wed May 16 00:00:00 EDT 2012

Induction of cardiac myogenic lineage development differs between mesenchymal and satellite cells and is accelerated by bone morphogenetic protein-4.

Journal of molecular and cellular cardiology

Grajales L,García J,Geenen DL

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2012

Innate lymphoid cells mediate influenza-induced airway hyper-reactivity independently of adaptive immunity.

Nature immunology

Chang YJ,Kim HY,Albacker LA,Baumgarth N,McKenzie AN,Smith DE,Dekruyff RH,Umetsu DT

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Sun May 29 00:00:00 EDT 2011

Essential roles for Pot1b in HSC self-renewal and survival.

Blood

Wang Y,Shen MF,Chang S

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2011

CD24-positive cells from normal adult mouse liver are hepatocyte progenitor cells.

Stem cells and development

Qiu Q,Hernandez JC,Dean AM,Rao PH,Darlington GJ

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2011

A high omega-3 fatty acid diet has different effects on early and late stage myeloid progenitors.

Lipids

Varney ME,Buchanan JT,Dementieva Y,Hardman WE,Sollars VE

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Sat Jan 01 00:00:00 EST 2011

Delayed enrichment of mesenchymal cells promotes cardiac lineage and calcium transient development.

Journal of molecular and cellular cardiology

Grajales L,García J,Banach K,Geenen DL

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2010

Wnt7a activates the planar cell polarity pathway to drive the symmetric expansion of satellite stem cells.

Cell stem cell

Le Grand F,Jones AE,Seale V,Scimè A,Rudnicki MA

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Fri Jun 05 00:00:00 EDT 2009

Reporter gene-expressing bone marrow-derived stromal cells are immune-tolerated following implantation in the central nervous system of syngeneic immunocompetent mice.

BMC biotechnology

Bergwerf I,De Vocht N,Tambuyzer B,Verschueren J,Reekmans K,Daans J,Ibrahimi A,Van Tendeloo V,Chatterjee S,Goossens H,Jorens PG,Baekelandt V,Ysebaert D,Van Marck E,Berneman ZN,Linden AV,Ponsaerts P

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 61-5981-82) in Flow Cytometry

Wed Jan 07 00:00:00 EST 2009

Bioinformatics

Protein Aliases: locus A/E; Locus AE; Ly-6A.2/Ly-6E.1; Ly-6E; ly6a-e; Ly6A/E; lymphocyte antigen 6 complex; Lymphocyte antigen 6A-2/6E-1; Lymphocyte antigen 6E; SCA-1; Stem cell antigen 1; Stem cell antigen 2; T-cell-activating protein; TAP; Thymic shared antigen 1; TSA-1

Gene Aliases: 9804; Ly-6A.2; Ly-6A/E; Ly-6E.1; Ly6; Ly67; Ly6a; Ly6e; RIG-E; Sca-1; Sca-2; Sca1; TAP; TSA-1; Tsa1

UniProt ID: (Mouse) P05533, (Mouse) Q64253

Entrez Gene ID: (Mouse) 110454, (Mouse) 17069

Function(s)

in utero embryonic development adrenal gland development organ growth norepinephrine metabolic process epinephrine secretion ventricular cardiac muscle tissue morphogenesis

Ly-6A/E (Sca-1) Monoclonal Antibody (D7), PE-eFluor™ 610, eBioscience™

Ly-6A/E (Sca-1) Antibody (61-5981-82) in Flow

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61-5981-82

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