Invitrogen

Ly-6A/E (Sca-1) Monoclonal Antibody (D7), Super Bright™ 436, eBioscience™

Product Image Gallery

Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow) — Staining of C57Bl/6 bone marrow cells with Anti-Mouse CD117 (c-Kit) PE (Product # 12-1172-82) and 0.125 µg of Rat IgG2a K Isotype Control Super Bright 436 (Product # 62-4321-82) (left) or 0.125 µg of Anti-Mouse Ly-6A/E (Sca-1) Super Bright 436 (right). Cells in the large scatter population were used for analysis.

Ly-6A/E (Sca-1) Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC) — Additional file 4: Figure S4. Expression of CD117 and Sca1 in trophoblast cells. Trophoblast cells (TCs) were isolated from embryonic day 18.5 placentas using a percoll gradient, and expanded in growth medium. Sca1 antibody conjugated with fluorescein isothiocyanate (FITC, eBioscience, Cat. 11-5981, 1:100) and CD117 antibody conjugated with allophycocyanin (APC, BD Pharmingen, Cat. 553356, 1:10) were incubated with the TCs at 4oC for 30 min in darkness. DAPI was added to stain nuclei following fixation of the cells, and then a cytospin of the cells was performed onto a slide. Images were taken by confocal microscopy, with a lower power image on the top row, and cells within the white boxes depicted in a higher power image on the lower row. Representative images showed that the majority of TCs were positive for Sca1 (green, left and right columns). A subpopulation of Sca1+ cells also expressed CD117 (red, middle and right columns). Right column showed merged images of Sca1, CD117 and DAPI (blue).

Ly-6A/E (Sca-1) Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow) — Fig. 5 SCF from AECs regulates HSCs. a Representative images of femur from Bmx -Cre ERT2 ;iTdtomato; Nestin -GFP mice stained with anti-VE-cadherin and anti-CD31. All the panels show the same field for different channels. Scale bar, 100 mum. b Enlarged image of the field highlighted by the yellow rectangle in ( a ). All panels show the same field for different channels. The Bmx -Cre ERT2 -labelled cells colocalise with Nestin -GFP-labelled cells. Scale bar, 10 mum. c Image of whole-mount sternal BM from Bmx -Cre ERT2 ;iTdtomato injected with anti-Sca-1. Scale bar, 50 mum. d Representative FACS plot of the labelling of AECs and SECs by Bmx -Cre ERT2 . e Quantification of the labelling efficiency of AECs and SECs by Bmx -Cre ERT2 . Data are represented as mean +- SEM. n = 8 for AECs and SECs. f Representative FACS plot of Nestin -GFP + MSCs labelling by Bmx -Cre ERT2 . g Percentage of HSCs (CD150 + CD48 - LSK) in the BM. Data are represented as mean +- SEM. n = 11 for Cre (-) and n = 14 for Cre (+). h Number of HSCs (CD150 + CD48 - LSK) in the BM. Data are represented as mean +- SEM. n = 11 for Cre (-) and n = 14 for Cre (+). i Percentage of LSK in the BM. Data are represented as mean +- SEM. n = 11 for Cre (-) and n = 14 for Cre (+). j Number of LSK in the BM. Data are represented as mean +- SEM. n = 11 for Cre (-) and n = 14 for Cre (+). k - n Percentage of donor-derived cells after competitive transplantation. n = 6 mice

Ly-6A/E (Sca-1) Antibody in Immunocytochemistry (ICC/IF) — Fig. 3 Elevated cell adhesion molecule expression on SECs. a qPCR analysis of adhesion molecules, Selp , Sele , Icam1 , and Vcam1 of sorted AECs and SECs. Data are represented as mean +- SEM. n = 4 for AECs and SECs. Data were analysed with two-tailed t -test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. b PDPN can be substituted for antibody against the adhesion molecule ICAM1 and E-selectin. Shown is EC isolation from a wild-type mouse injected i.v. with anti-PDPN and the enzymatically dissociated bone marrow cells were stained with other antibodies ex vivo and pre-gated on singlet, live, CD45 - , Ter119 - , CD31 + cells. c Anti-CD31 is dispensable for the analysis of bone marrow AECs and SECs. Flushed enzymatically dissociated bone marrow cells were stained with anti-CD45, anti-Ter119, anti-ICAM-1/anti-CD62E, anti-CD31, and anti-Sca-1 ex vivo. Analysis was pre-gated on singlet, live, CD45 - , Ter119 - cells. d P-selectin marks sinusoids. Anti-P-selectin and anti-CD31/CD144 were i.v. injected into Nestin -GFP mice and femur was imaged after removal. Scale bar, 50 um. e ICAM-1 marks sinusoids. Anti-ICAM-1 and anti-CD31/CD144 were i.v. injected into Nestin -GFP mice and femur was imaged after harvest. Scale bar, 100 um. f E-selectin marks sinusoids. Anti-E-selectin, anti-Sca-1, and anti-CD31/CD144 were i.v. injected into wild-type mice and femur was imaged after harvest. Scale bar, 50 um

Ly-6A/E (Sca-1) Antibody in Immunohistochemistry (IHC) — Fig. 1 Isolation and characterization of mesenchymal stem cells. a Schematic showing isolation of mouse bone marrow cells (BMCs) and its properties of self-renewal and differentiation of MSCs. b Representative microscopic images displaying isolated bone marrow cells that show circular and round-shaped morphology of BMCs; whereas, adhered MSCs show fibroblast or eye-shaped morphology. c Immunofluorescence images of MSCs showing the expression of MSC-positive markers (CD29, CD44, and SCA1). d Immunophenotypic characterization of MSCs using flow cytometer showing the absolute counts of MSC positive markers such as CD29, CD44, and SCA1, as well as expression of MSC-negative (CD34, CD45, and CD11b). Isotype controls for each antibody were used as controls and FITC/rhodamine-conjugated secondary antibodies were used for their detection. e Bar graph (quantification from four independent experiments (expressed as mean +- standard error)) showing the percentage of cells expressing the MSC-positive and negative markers respectively. f , g Immunophenotypic characterization of BMCs using flow cytometer showing expression various markers (CD29, CD44, CD45, and CD11b). Bar graph expressed as mean +- standard error shows quantification of respective markers from three independent experiments.

Product Details

62-5981-82

Applications	Tested Dilution	Publications
Immunohistochemistry (IHC)	-	View 6 publications 6 publications
Immunocytochemistry (ICC/IF)	-	View 4 publications 4 publications
Flow Cytometry (Flow)	0.25 µg/test	View 48 publications 48 publications

Product Specifications
Species Reactivity	Mouse
Published species	Fish, Mouse
Host/Isotype	Rat / IgG2a, kappa
Recommended Isotype Control	Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 436, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	D7
Conjugate	Super Bright™ 436 Alexa Fluor 700 APC Biotin Brilliant UV 737 Brilliant UV 805 FITC PE PE-Cyanine5 PE-Cyanine7 PE-eFluor 610 PerCP-Cyanine5.5 Super Bright 600 Super Bright 645 Unconjugated
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with BSA
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2637287

Product Specific Information

Description: The D7 monoclonal antibody reacts with mouse Sca-1, an 18 kDa member of the Ly-6 family of GPI-linked surface proteins. D7 reacts with both Ly-6E.1 and Ly-6A.2 molecules expressed by mouse hematopoietic stem cells, myeloid population and peripheral T and B cells. Non-activated splenocytes from Ly-6.2 strains including C57BL, SJL, 129, AKR, and others demonstrate higher frequency of D7-positive cells compared to the Ly-6.1 strains such as BALB/c, C3H, NZB and others. Sca-1 is expressed at high levels upon activation regardless of the Ly-6 haplotype. The Ly-6 family is involved in regulation and function of T cell activation. Sca-1 is a major phenotypic marker for mouse hematopoietic progenitor/stem cell subset.

Applications Reported: This D7 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This D7 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

Stem Cell Antigen-1 (Sca-1) is a member of the lymphocyte antigen 6 (Ly-6) family. It is expressed on multipotent hematopoietic stem cells (HSC) and is used as a marker of HSC in mice. Sca-1 is used in combination with lineage depletion antibodies to identify murine HSC. Sca-1 can be found in fetal liver, adult bone marrow, and adult peripheral blood and spleen. Sca-1 may be involved in the regulation of B and T cell activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

Differential activation of Ca²⁺ influx channels modulate stem cell potency, their proliferation/viability and tissue regeneration.

NPJ Regenerative medicine

Ahamad N,Sun Y,Nascimento Da Conceicao V,Xavier Paul Ezhilan CRD,Natarajan M,Singh BB

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Immunohistochemistry

Wed Oct 20 00:00:00 EDT 2021

XIAOPI formula inhibits the pre-metastatic niche formation in breast cancer via suppressing TAMs/CXCL1 signaling.

Cell communication and signaling : CCS

Zheng Y,Wang N,Wang S,Yang B,Situ H,Zhong L,Lin Y,Wang Z

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Thu Mar 26 00:00:00 EDT 2020

Multipotency of mouse trophoblast stem cells.

Stem cell research & therapy

Hou M,Han J,Li G,Kwon MY,Jiang J,Emani S,Taglauer ES,Park JA,Choi EB,Vodnala M,Fong YW,Emani SM,Rosas IO,Perrella MA,Liu X

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Immunocytochemistry

Thu Feb 13 00:00:00 EST 2020

Multipotency of mouse trophoblast stem cells.

Stem cell research & therapy

Hou M,Han J,Li G,Kwon MY,Jiang J,Emani S,Taglauer ES,Park JA,Choi EB,Vodnala M,Fong YW,Emani SM,Rosas IO,Perrella MA,Liu X

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Immunocytochemistry

Thu Feb 13 00:00:00 EST 2020

Multipotency of mouse trophoblast stem cells.

Stem cell research & therapy

Hou M,Han J,Li G,Kwon MY,Jiang J,Emani S,Taglauer ES,Park JA,Choi EB,Vodnala M,Fong YW,Emani SM,Rosas IO,Perrella MA,Liu X

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Immunocytochemistry

Thu Feb 13 00:00:00 EST 2020

PDGFRA defines the mesenchymal stem cell Kaposi's sarcoma progenitors by enabling KSHV oncogenesis in an angiogenic environment.

PLoS pathogens

Naipauer J,Rosario S,Gupta S,Premer C,Méndez-Solís O,Schlesinger M,Ponzinibbio V,Jain V,Gay L,Renne R,Chan HL,Morey L,Salyakina D,Abba M,Williams S,Hare JM,Goldschmidt-Clermont PJ,Mesri EA

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Sun Dec 01 00:00:00 EST 2019

Procr-expressing progenitor cells are responsible for murine ovulatory rupture repair of ovarian surface epithelium.

Nature communications

Wang J,Wang D,Chu K,Li W,Zeng YA

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Thu Oct 31 00:00:00 EDT 2019

Macrophages fine tune satellite cell fate in dystrophic skeletal muscle of mdx mice.

PLoS genetics

Madaro L,Torcinaro A,De Bardi M,Contino FF,Pelizzola M,Diaferia GR,Imeneo G,Bouchè M,Puri PL,De Santa F

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2019

Bone marrow adipose tissue-derived stem cell factor mediates metabolic regulation of hematopoiesis.

Haematologica

Zhang Z,Huang Z,Ong B,Sahu C,Zeng H,Ruan HB

62-5981-82 was used in Flow Cytometry to demonstrate that marrow adipose tissue provides stem cell factor essentially for hematopoiesis both at the steady state and upon metabolic stresses.

Sun Sep 01 00:00:00 EDT 2019

Excessive exosome release is the pathogenic pathway linking a lysosomal deficiency to generalized fibrosis.

Science advances

van de Vlekkert D,Demmers J,Nguyen XX,Campos Y,Machado E,Annunziata I,Hu H,Gomero E,Qiu X,Bongiovanni A,Feghali-Bostwick CA,d'Azzo A

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Mon Jul 01 00:00:00 EDT 2019

The microRNAs miR-204 and miR-211 maintain joint homeostasis and protect against osteoarthritis progression.

Nature communications

Huang J,Zhao L,Fan Y,Liao L,Ma PX,Xiao G,Chen D

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Fri Jun 28 00:00:00 EDT 2019

TGFβ-induced degradation of TRAF3 in mesenchymal progenitor cells causes age-related osteoporosis.

Nature communications

Li J,Ayoub A,Xiu Y,Yin X,Sanders JO,Mesfin A,Xing L,Yao Z,Boyce BF

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Wed Jun 26 00:00:00 EDT 2019

Differential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus.

eLife

Moretti FA,Klapproth S,Ruppert R,Margraf A,Weber J,Pick R,Scheiermann C,Sperandio M,Fässler R,Moser M

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Thu Sep 06 00:00:00 EDT 2018

Stem cell factor is selectively secreted by arterial endothelial cells in bone marrow.

Nature communications

Xu C,Gao X,Wei Q,Nakahara F,Zimmerman SE,Mar J,Frenette PS

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Fri Jun 22 00:00:00 EDT 2018

Stem cell factor is selectively secreted by arterial endothelial cells in bone marrow.

Nature communications

Xu C,Gao X,Wei Q,Nakahara F,Zimmerman SE,Mar J,Frenette PS

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Fri Jun 22 00:00:00 EDT 2018

The H3K4 methyltransferase Setd1b is essential for hematopoietic stem and progenitor cell homeostasis in mice.

eLife

Schmidt K,Zhang Q,Tasdogan A,Petzold A,Dahl A,Arneth BM,Slany R,Fehling HJ,Kranz A,Stewart AF,Anastassiadis K

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Tue Jun 19 00:00:00 EDT 2018

Longitudinal intravital imaging of the femoral bone marrow reveals plasticity within marrow vasculature.

Nature communications

Reismann D,Stefanowski J,Günther R,Rakhymzhan A,Matthys R,Nützi R,Zehentmeier S,Schmidt-Bleek K,Petkau G,Chang HD,Naundorf S,Winter Y,Melchers F,Duda G,Hauser AE,Niesner RA

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Immunofluorescence

Mon Dec 18 00:00:00 EST 2017

Microgrooved-surface topography enhances cellular division and proliferation of mouse bone marrow-derived mesenchymal stem cells.

PloS one

Chaudhary JK,Rath PC

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Thu Oct 19 00:00:00 EDT 2017

Zoledronate suppressed angiogenesis and osteogenesis by inhibiting osteoclasts formation and secretion of PDGF-BB.

PloS one

Gao SY,Zheng GS,Wang L,Liang YJ,Zhang SE,Lao XM,Li K,Liao GQ

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Mon Sep 18 00:00:00 EDT 2017

FOXP1 controls mesenchymal stem cell commitment and senescence during skeletal aging.

The Journal of clinical investigation

Li H,Liu P,Xu S,Li Y,Dekker JD,Li B,Fan Y,Zhang Z,Hong Y,Yang G,Tang T,Ren Y,Tucker HO,Yao Z,Guo X

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Mon Apr 03 00:00:00 EDT 2017

GATA4-dependent organ-specific endothelial differentiation controls liver development and embryonic hematopoiesis.

The Journal of clinical investigation

Géraud C,Koch PS,Zierow J,Klapproth K,Busch K,Olsavszky V,Leibing T,Demory A,Ulbrich F,Diett M,Singh S,Sticht C,Breitkopf-Heinlein K,Richter K,Karppinen SM,Pihlajaniemi T,Arnold B,Rodewald HR,Augustin HG,Schledzewski K,Goerdt S

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2017

Induced p53 loss in mouse luminal cells causes clonal expansion and development of mammary tumours.

Nature communications

Tao L,Xiang D,Xie Y,Bronson RT,Li Z

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Mon Feb 13 00:00:00 EST 2017

Mutant U2AF1-expressing cells are sensitive to pharmacological modulation of the spliceosome.

Nature communications

Shirai CL,White BS,Tripathi M,Tapia R,Ley JN,Ndonwi M,Kim S,Shao J,Carver A,Saez B,Fulton RS,Fronick C,O'Laughlin M,Lagisetti C,Webb TR,Graubert TA,Walter MJ

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Mon Jan 09 00:00:00 EST 2017

Bone marrow-derived immature myeloid cells are a main source of circulating suPAR contributing to proteinuric kidney disease.

Nature medicine

Hahm E,Wei C,Fernandez I,Li J,Tardi NJ,Tracy M,Wadhwani S,Cao Y,Peev V,Zloza A,Lusciks J,Hayek SS,O'Connor C,Bitzer M,Gupta V,Sever S,Sykes DB,Scadden DT,Reiser J

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Sun Jan 01 00:00:00 EST 2017

Identification of a CD133-CD55- population functions as a fetal common skeletal progenitor.

Scientific reports

Weng L,Hu X,Kumar B,Garcia M,Todorov I,Jung X,Marcucci G,Forman SJ,Chen CC

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Immunofluorescence

Thu Dec 08 00:00:00 EST 2016

Identification of an epidermal keratinocyte AMPA glutamate receptor involved in dermatopathies associated with sensory abnormalities.

Pain reports

Cabañero D,Irie T,Celorrio M,Trousdale C,Owens DM,Virley D,Albrecht PJ,Caterina MJ,Rice FL,Morón JA

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Thu Sep 01 00:00:00 EDT 2016

The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity.

Cell

Damgaard RB,Walker JA,Marco-Casanova P,Morgan NV,Titheradge HL,Elliott PR,McHale D,Maher ER,McKenzie ANJ,Komander D

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Thu Aug 25 00:00:00 EDT 2016

Cardiac-Specific Activation of IKK2 Leads to Defects in Heart Development and Embryonic Lethality.

PloS one

Kraut B,Maier HJ,Kókai E,Fiedler K,Boettger T,Illing A,Kostin S,Walther P,Braun T,Wirth T

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Immunofluorescence

Mon Jun 13 00:00:00 EDT 2016

Hedgehog-driven myogenic tumors recapitulate skeletal muscle cellular heterogeneity.

Experimental cell research

Hettmer S,Lin MM,Tchessalova D,Tortorici SJ,Castiglioni A,Desai T,Mao J,McMahon AP,Wagers AJ

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2016

Cis-regulatory mechanisms governing stem and progenitor cell transitions.

Science advances

Johnson KD,Kong G,Gao X,Chang YI,Hewitt KJ,Sanalkumar R,Prathibha R,Ranheim EA,Dewey CN,Zhang J,Bresnick EH

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2015

Cellular and molecular characterization of human cardiac stem cells reveals key features essential for their function and safety.

Stem cells and development

Vahdat S,Mousavi SA,Omrani G,Gholampour M,Sotoodehnejadnematalahi F,Ghazizadeh Z,Gharechahi J,Baharvand H,Salekdeh GH,Aghdami N

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Mon Jun 15 00:00:00 EDT 2015

KLF4-dependent phenotypic modulation of smooth muscle cells has a key role in atherosclerotic plaque pathogenesis.

Nature medicine

Shankman LS,Gomez D,Cherepanova OA,Salmon M,Alencar GF,Haskins RM,Swiatlowska P,Newman AA,Greene ES,Straub AC,Isakson B,Randolph GJ,Owens GK

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2015

KLF4-dependent phenotypic modulation of smooth muscle cells has a key role in atherosclerotic plaque pathogenesis.

Nature medicine

Shankman LS,Gomez D,Cherepanova OA,Salmon M,Alencar GF,Haskins RM,Swiatlowska P,Newman AA,Greene ES,Straub AC,Isakson B,Randolph GJ,Owens GK

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2015

Amniotic fluid stem cells prevent follicle atresia and rescue fertility of mice with premature ovarian failure induced by chemotherapy.

PloS one

Xiao GY,Liu IH,Cheng CC,Chang CC,Lee YH,Cheng WT,Wu SC

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Thu May 28 00:00:00 EDT 2015

Multi-Drug Resistance ABC Transporter Inhibition Enhances Murine Ventral Prostate Stem/Progenitor Cell Differentiation.

Stem cells and development

Samant MD,Jackson CM,Felix CL,Jones AJ,Goodrich DW,Foster BA,Huss WJ

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Fri May 15 00:00:00 EDT 2015

Rapid depot-specific activation of adipocyte precursor cells at the onset of obesity.

Nature cell biology

Jeffery E,Church CD,Holtrup B,Colman L,Rodeheffer MS

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2015

Limited miR-17-92 overexpression drives hematologic malignancies.

Leukemia research

Danielson LS,Reavie L,Coussens M,Davalos V,Castillo-Martin M,Guijarro MV,Coffre M,Cordon-Cardo C,Aifantis I,Ibrahim S,Liu C,Koralov SB,Hernando E

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Sun Mar 01 00:00:00 EST 2015

Recruitment of bone marrow-derived cells to periodontal tissue defects.

Frontiers in cell and developmental biology

Kimura Y,Komaki M,Iwasaki K,Sata M,Izumi Y,Morita I

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Mon Nov 03 00:00:00 EST 2014

Evidence for Transfer of Membranes from Mesenchymal Stem Cells to HL-1 Cardiac Cells.

Stem cells international

Boomsma RA,Geenen DL

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Wed Oct 08 00:00:00 EDT 2014

WASH is required for the differentiation commitment of hematopoietic stem cells in a c-Myc-dependent manner.

The Journal of experimental medicine

Xia P,Wang S,Huang G,Zhu P,Li M,Ye B,Du Y,Fan Z

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Mon Sep 22 00:00:00 EDT 2014

Cardiogenic genes expressed in cardiac fibroblasts contribute to heart development and repair.

Circulation research

Furtado MB,Costa MW,Pranoto EA,Salimova E,Pinto AR,Lam NT,Park A,Snider P,Chandran A,Harvey RP,Boyd R,Conway SJ,Pearson J,Kaye DM,Rosenthal NA

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Fri Apr 25 00:00:00 EDT 2014

Immunohistological localization of endogenous unlabeled stem cells in wounded skin.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Hong S,Alapure BV,Lu Y,Tian H,Wang Q

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Immunofluorescence

Tue Apr 01 00:00:00 EDT 2014

Cell-autonomous heparanase modulates self-renewal and migration in bone marrow-derived mesenchymal stem cells.

Journal of biomedical science

Cheng CC,Lee YH,Lin SP,Huangfu WC,Liu IH

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Thu Mar 13 00:00:00 EDT 2014

Validation of the effects of TGF-β1 on tumor recurrence and prognosis through tumor retrieval and cell mechanical properties.

Cancer cell international

Wu TH,Chou YW,Chiu PH,Tang MJ,Hu CW,Yeh ML

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Mon Mar 03 00:00:00 EST 2014

Overexpressing sonic hedgehog peptide restores periosteal bone formation in a murine bone allograft transplantation model.

Molecular therapy : the journal of the American Society of Gene Therapy

Huang C,Tang M,Yehling E,Zhang X

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Sat Feb 01 00:00:00 EST 2014

A mouse model for inducible overexpression of Prdm14 results in rapid-onset and highly penetrant T-cell acute lymphoblastic leukemia (T-ALL).

Disease models & mechanisms

Carofino BL,Ayanga B,Justice MJ

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2013

Cis-element mutated in GATA2-dependent immunodeficiency governs hematopoiesis and vascular integrity.

The Journal of clinical investigation

Johnson KD,Hsu AP,Ryu MJ,Wang J,Gao X,Boyer ME,Liu Y,Lee Y,Calvo KR,Keles S,Zhang J,Holland SM,Bresnick EH

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Mon Oct 01 00:00:00 EDT 2012

Induction of cardiac myogenic lineage development differs between mesenchymal and satellite cells and is accelerated by bone morphogenetic protein-4.

Journal of molecular and cellular cardiology

Grajales L,García J,Geenen DL

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2012

CTC1 deletion results in defective telomere replication, leading to catastrophic telomere loss and stem cell exhaustion.

The EMBO journal

Gu P,Min JN,Wang Y,Huang C,Peng T,Chai W,Chang S

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Wed May 16 00:00:00 EDT 2012

Lunatic fringe deficiency cooperates with the Met/Caveolin gene amplicon to induce basal-like breast cancer.

Cancer cell

Xu K,Usary J,Kousis PC,Prat A,Wang DY,Adams JR,Wang W,Loch AJ,Deng T,Zhao W,Cardiff RD,Yoon K,Gaiano N,Ling V,Beyene J,Zacksenhaus E,Gridley T,Leong WL,Guidos CJ,Perou CM,Egan SE

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Tue May 15 00:00:00 EDT 2012

Essential roles for Pot1b in HSC self-renewal and survival.

Blood

Wang Y,Shen MF,Chang S

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2011

CD24-positive cells from normal adult mouse liver are hepatocyte progenitor cells.

Stem cells and development

Qiu Q,Hernandez JC,Dean AM,Rao PH,Darlington GJ

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2011

Innate lymphoid cells mediate influenza-induced airway hyper-reactivity independently of adaptive immunity.

Nature immunology

Chang YJ,Kim HY,Albacker LA,Baumgarth N,McKenzie AN,Smith DE,Dekruyff RH,Umetsu DT

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Sun May 29 00:00:00 EDT 2011

A high omega-3 fatty acid diet has different effects on early and late stage myeloid progenitors.

Lipids

Varney ME,Buchanan JT,Dementieva Y,Hardman WE,Sollars VE

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Sat Jan 01 00:00:00 EST 2011

Delayed enrichment of mesenchymal cells promotes cardiac lineage and calcium transient development.

Journal of molecular and cellular cardiology

Grajales L,García J,Banach K,Geenen DL

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2010

Wnt7a activates the planar cell polarity pathway to drive the symmetric expansion of satellite stem cells.

Cell stem cell

Le Grand F,Jones AE,Seale V,Scimè A,Rudnicki MA

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Fri Jun 05 00:00:00 EDT 2009

Reporter gene-expressing bone marrow-derived stromal cells are immune-tolerated following implantation in the central nervous system of syngeneic immunocompetent mice.

BMC biotechnology

Bergwerf I,De Vocht N,Tambuyzer B,Verschueren J,Reekmans K,Daans J,Ibrahimi A,Van Tendeloo V,Chatterjee S,Goossens H,Jorens PG,Baekelandt V,Ysebaert D,Van Marck E,Berneman ZN,Linden AV,Ponsaerts P

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Wed Jan 07 00:00:00 EST 2009

Reporter gene-expressing bone marrow-derived stromal cells are immune-tolerated following implantation in the central nervous system of syngeneic immunocompetent mice.

BMC biotechnology

Bergwerf I,De Vocht N,Tambuyzer B,Verschueren J,Reekmans K,Daans J,Ibrahimi A,Van Tendeloo V,Chatterjee S,Goossens H,Jorens PG,Baekelandt V,Ysebaert D,Van Marck E,Berneman ZN,Linden AV,Ponsaerts P

Published figure using Ly-6A/E (Sca-1) monoclonal antibody (Product # 62-5981-82) in Flow Cytometry

Wed Jan 07 00:00:00 EST 2009

Bioinformatics

Protein Aliases: locus A/E; Locus AE; Ly-6A.2/Ly-6E.1; Ly-6E; ly6a-e; Ly6A/E; lymphocyte antigen 6 complex; Lymphocyte antigen 6A-2/6E-1; Lymphocyte antigen 6E; SCA-1; Stem cell antigen 1; Stem cell antigen 2; T-cell-activating protein; TAP; Thymic shared antigen 1; TSA-1

Gene Aliases: 9804; Ly-6A.2; Ly-6A/E; Ly-6E.1; Ly6; Ly67; Ly6a; Ly6e; RIG-E; Sca-1; Sca-2; Sca1; TAP; TSA-1; Tsa1

UniProt ID: (Mouse) P05533, (Mouse) Q64253

Entrez Gene ID: (Mouse) 110454, (Mouse) 17069

Function(s)

in utero embryonic development adrenal gland development organ growth norepinephrine metabolic process epinephrine secretion ventricular cardiac muscle tissue morphogenesis