Invitrogen

Ly-6A/E (Sca-1) Monoclonal Antibody (D7), Super Bright 436, eBioscience™

Product Image Gallery

Ly-6A/E (Sca-1) Antibody — Figure 3. Reduced frequencies of T cell progenitors in Fermt3 -/- thymi. ( A ) Thymocytes from Fermt3 +/+ and Fermt3 -/- mice were stained for lineage markers (B220, CD19, TER119, NK1.1, CD11b, Gr-1, CD8alpha, CD3e, TCRbeta, TCRgammadelta and CD11c), CD44 and c-kit to identify DN 1-2 (Lin neg , c-kit hi , CD44 hi ) and the DN 3-4 (Lin neg , c-kit low , CD44 low ) populations. ( B and C ) PB from Fermt3 +/+ and Fermt3 -/- animals ( P3 ) were stained for lineage markers (TER119, B220, CD11b, Gr-1, CD11c, NK1.1, CD4 and CD8alpha), c-kit and Sca-1 to identify hematopoietic progenitor cells and analysed by flow cytometry. N = 4. Numbers within the representative FACS plots indicate cell percentages. Bars indicate means +- standard errors. **p < 0.01; ***p < 0.001. See also Figure 3--figure supplement 1 . Figure 3--figure supplement 1. Reduced frequencies of T cell prognitors in Fermt3 -/- thymi. Thymocytes from Fermt3 +/+ and Fermt3 -/- P3 mice were stained for lineage markers (B220, CD19, TER119, NK1.1, CD11b, Gr-1, CD8alpha, CD3epsilon, TCRbeta, TCRgammadelta and CD11c), CD44 and CD25 to identify and quantify DN1 (Lin neg , CD44 hi , CD25 - ), DN2 (Lin neg , CD44 hi , CD25 + ), DN3 (Lin neg , CD44 low , CD25 + ) and DN4 (Lin neg , CD44 low , CD25 - ) populations by flow cytometry. Numbers within FACS plots indicate cell percentages. Total number of DN1, DN2, DN3 and DN4 cells were measured by flow cytometry. N = 20. Bars indicate means +- stand

Ly-6A/E (Sca-1) Antibody in Flow Cytometry (Flow) — Staining of C57Bl/6 bone marrow cells with Anti-Mouse CD117 (c-Kit) PE (Product # 12-1172-82) and 0.125 µg of Rat IgG2a K Isotype Control Super Bright 436 (Product # 62-4321-82) (left) or 0.125 µg of Anti-Mouse Ly-6A/E (Sca-1) Super Bright 436 (right). Cells in the large scatter population were used for analysis.

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

0.25 µg/test

Published Applications

Flow Cytometry (Flow)

See 28 publications below

Immunofluorescence (IF)

See 6 publications below

Immunohistochemistry (IHC)

See 2 publications below

Product Specifications

Species Reactivity

Mouse

Published species

Fish

Host / Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Super Bright 436, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

Conjugate

Super Bright 436

Excitation/Emission Profile

View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

RRID

AB_2637287

Product Specific Information

Description: The D7 monoclonal antibody reacts with mouse Sca-1, an 18 kDa member of the Ly-6 family of GPI-linked surface proteins. D7 reacts with both Ly-6E.1 and Ly-6A.2 molecules expressed by mouse hematopoietic stem cells, myeloid population and peripheral T and B cells. Non-activated splenocytes from Ly-6.2 strains including C57BL, SJL, 129, AKR, and others demonstrate higher frequency of D7-positive cells compared to the Ly-6.1 strains such as BALB/c, C3H, NZB and others. Sca-1 is expressed at high levels upon activation regardless of the Ly-6 haplotype. The Ly-6 family is involved in regulation and function of T cell activation. Sca-1 is a major phenotypic marker for mouse hematopoietic progenitor/stem cell subset.

Applications Reported: This D7 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This D7 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

Ly-6A/E molecules are implicated in the regulation of T cell activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Ly-6A.2/Ly-6E.1; Ly-6E; Lymphocyte antigen 6A-2/6E-1; Lymphocyte antigen 6E; SCA-1; Stem cell antigen 1; Stem cell antigen 2; T-cell-activating protein; TAP; Thymic shared antigen 1; TSA-1

Gene Aliases: 9804; Ly-6A.2; Ly-6A/E; Ly-6E.1; Ly6; Ly67; Ly6a; Ly6e; RIG-E; Sca-1; Sca-2; Sca1; TAP; TSA-1; Tsa1

UniProt ID: (Mouse) P05533, (Mouse) Q64253

Entrez Gene ID: (Mouse) 110454, (Mouse) 17069

Function(s)

in utero embryonic development adrenal gland development organ growth norepinephrine metabolic process epinephrine secretion ventricular cardiac muscle tissue morphogenesis