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Invitrogen
Ly-6D Monoclonal Antibody (49-H4), eFluor™ 450, eBioscience™
Product Details
48-5974-80
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2c, kappa
Class
Monoclonal
Type
Antibody
Clone
49-H4
Conjugate
Excitation/Emission Max
405/445 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2574089
Product Specific Information
Description: This 49-H4 monoclonal antibody reacts with mouse Ly-6D, which is also known as ThB. This member of the Ly6 family of GPI-anchored cell surface glycoproteins is expressed on thymocytes and splenic B cells. Moreover, common lymphoid progenitor cells that express Ly-6D have been shown to commit to the B cell lineage. Expression of Ly-6D has also been associated with Rag-1 expression along with a reduced ability to differentiate into T or NK cells. Finally, reports indicate that the level of Ly-6D expression varies in different mouse strains.^M
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Applications Reported: This 49-H4 antibody has been reported for use in flow cytometric analysis.^M
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Applications Tested: This 49-H4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.^M
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eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.^M
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Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.^M
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Filtration: 0.2 µm post-manufacturing filtered.
Target Information
May act as a specification marker at earliest stage specification of lymphocytes between B- and T-cell development. Marks the earliest stage of B-cell specification. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: Ly-6D; Lymphocyte antigen 6D; ThB; Thymocyte B-cell antigen
Gene Aliases: Ly-61; Ly61; Ly6d; Thb
UniProt ID: (Mouse) P35459
Entrez Gene ID: (Mouse) 17068
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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