Invitrogen

Lyn Monoclonal Antibody (LYN-01)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

View all (40) Lyn antibodies

Questions & Answers

Questions & Answers

Product Image Gallery

Lyn Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Tyrosine-protein kinase Lyn was performed using 70% confluent log phase A431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Lyn Monoclonal Antibody (LYN-01) (Product # MA1-19334) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 40X magnification.

Lyn Antibody in Western Blot (WB) — Western Blot was performed using Anti-Lyn Monoclonal Antibody (LYN-01) (Product # MA1-19334) and a 58 kDa band corresponding to Tyrosine-protein kinase Lyn was observed across cell lines and tissues with uncharacterized band(*). Whole cell extracts (30 µg lysate) of K-562 (Lane 1), Raji (Lane 2), Jurkat (Lane 3), Mouse Spleen (Lane 4), Rat Spleen (Lane 5), Mouse Brain (Lane 6), Rat Brain (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Lyn Antibody in Western Blot (WB) — Knockdown of Tyrosine-protein kinase Lyn was achieved by transfecting K-562 with Tyrosine-protein kinase Lyn specific siRNAs (Silencer® select Product # S8356, S8355). Western Blot analysis (Fig. a) was performed using Whole cell extracts from the Tyrosine-protein kinase Lyn knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The Blot was probed with Lyn Monoclonal Antibody (LYN-01) (Product # MA1-19334, 1:1000) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000). Densitometric analysis of this western Blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Tyrosine-protein kinase Lyn.

Lyn Antibody in Western Blot (WB) — Western blot analysis of Lyn using a monoclonal antibody (Product # MA1-19334).

Lyn Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. K-562 cells were transfected with Tyrosine-protein kinase Lyn siRNA and decrease in signal intensity was observed in Western Blot application using Anti-Lyn Monoclonal Antibody (LYN-01) (Product # MA1-19334). {KD}

Product Details

MA1-19334

Applications	Tested Dilution	Publications
Western Blot (WB)	1-2 µg/mL, 60 min	-
Immunocytochemistry (ICC/IF)	1:100	-
Immunoprecipitation (IP)	Assay-dependent	-

Product Specifications
Species Reactivity	Mouse, Rat, Human
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	LYN-01
Immunogen	Bacterially expressed recombinant fragment of human Lyn. (aa 8-238) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.4
Contains	15mM sodium azide
Storage conditions	4° C, do not freeze
RRID	AB_2265898

Target Information

Lyn proto oncogene (Lcl/Yes related novel protein tyrosine kinase) is encoded by the Lyn gene located on chromosome 8 in humans and belongs to the Src family kinase. It is a non-receptor tyrosine kinase that acts as a mediator in transducing cellular signaling. The most well studied expression of Lyn is in hematopoietic cell types, both lymphoid and myeloid cells with exclusion in T lymphocytes. It has also been observed that Lyn plays diverse roles such as in regulation of B cell receptor signaling, mast cell signaling and Epithelial - Mesenchymal Transition (EMT).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: FLJ26625; Lck/Yes-related novel protein tyrosine kinase; lyn protein non-receptor kinase; p53Lyn; p56Lyn; Tyrosine-protein kinase Lyn; V-yes-1 Yamaguchi sarcoma viral related oncogene homolog; Yamaguchi sarcoma viral (v-yes-1) oncogene homolog

Gene Aliases: AA407514; Hck-2; JTK8; LYN; p53Lyn; p56Lyn

UniProt ID: (Human) P07948, (Mouse) P25911, (Rat) Q07014

Entrez Gene ID: (Human) 4067, (Mouse) 17096, (Rat) 81515

B cell homeostasis regulation of cytokine production negative regulation of protein phosphorylation immune system process Fc receptor mediated stimulatory signaling pathway tolerance induction to self antigen histamine secretion by mast cell platelet degranulation negative regulation of myeloid leukocyte differentiation immune response-regulating cell surface receptor signaling pathway Fc receptor mediated inhibitory signaling pathway regulation of B cell apoptotic process protein phosphorylation inflammatory response cellular response to DNA damage stimulus signal transduction transmembrane receptor protein tyrosine kinase signaling pathway positive regulation of cell proliferation negative regulation of cell proliferation response to hormone positive regulation of neuron projection development phosphorylation peptidyl-tyrosine phosphorylation hemopoiesis erythrocyte differentiation positive regulation of cell migration negative regulation of B cell proliferation neuron projection development lipopolysaccharide-mediated signaling pathway regulation of mast cell activation regulation of cell adhesion mediated by integrin negative regulation of toll-like receptor 2 signaling pathway negative regulation of toll-like receptor 4 signaling pathway intracellular signal transduction peptidyl-tyrosine autophosphorylation positive regulation of phosphorylation positive regulation of tyrosine phosphorylation of STAT protein hemoglobin biosynthetic process regulation of mast cell degranulation negative regulation of MAP kinase activity positive regulation of phosphatidylinositol 3-kinase activity innate immune response regulation of erythrocyte differentiation protein autophosphorylation cytokine secretion regulation of cytokine secretion regulation of inflammatory response positive regulation of peptidyl-tyrosine phosphorylation B cell receptor signaling pathway regulation of B cell receptor signaling pathway positive regulation of B cell receptor signaling pathway positive regulation of cellular component movement regulation of release of sequestered calcium ion into cytosol positive regulation of glial cell proliferation positive regulation of Fc receptor mediated stimulatory signaling pathway positive regulation of stress-activated protein kinase signaling cascade regulation of ERK1 and ERK2 cascade negative regulation of ERK1 and ERK2 cascade positive regulation of oligodendrocyte progenitor proliferation negative regulation of mast cell proliferation positive regulation of mast cell proliferation cellular response to retinoic acid cellular response to peptide hormone stimulus regulation of monocyte chemotaxis regulation of platelet aggregation dendritic cell differentiation negative regulation of intracellular signal transduction positive regulation of dendritic cell apoptotic process regulation of protein phosphorylation stimulatory C-type lectin receptor signaling pathway adaptive immune response response to sterol depletion central nervous system development blood coagulation response to toxic substance response to carbohydrate oligodendrocyte development response to organic cyclic compound viral process signal transduction by protein phosphorylation platelet activation T cell costimulation cellular response to extracellular stimulus response to insulin cellular response to heat Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis response to drug response to amino acid ephrin receptor signaling pathway response to axon injury negative regulation of immune response leukocyte migration JAK-STAT cascade involved in growth hormone signaling pathway chemotaxis cell migration response to peptide hormone

Process(es)

protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity receptor binding platelet-derived growth factor receptor binding integrin binding protein binding ATP binding SH3 domain binding ubiquitin protein ligase binding gamma-tubulin binding glycosphingolipid binding ion channel binding phosphoprotein binding nucleotide binding protein kinase activity erythropoietin receptor binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups enzyme binding protein complex binding