|Tested species reactivity||Human, Mouse, Rat, Xenopus|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues L(348) N H T I L A G V H S N D H S V V(364) of human LPA1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-1041 detects lysophosphatidic acid receptor 1 protein in rat, human, mouse, and Xenopus samples.
PA1-1041 has successfully been used in Western blot procedures. By Western blot, this antibody detects an ~42 kDa protein representing lysophosphatidic acid receptor 1 in PC12 and HeLa whole cell lysates as well as mouse and Xenopus liver samples.
The PA1-1041 immunogen is a synthetic peptide corresponding to residues L(348) N H T I L A G V H S N D H S V V(364) of human LPA1. The immunizing sequence is completely conserved in human, mouse, rat and bovine. This peptide (Cat. # PEP-233) is available for use in neutralization and control experiments.
Lysophosphatidic acid receptor 1 (LPA1, EDG2, VZG-1) is a seven transmembrane receptor coupled to G proteins and has high affinity to lypophosphatidic acid (LPA). LPA1 is a member of the EDG-family of receptors which includes LPA1, LPA2 and LPA3.
These receptors mediate the serum borne lipid mediator LPA for a variety biological functions. The activation of LPA1 by LPA has shown to stimulate cell spreading and motility, mitogenesis, and various other nervous system activities.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.