|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide between amino acids 200-300 of the human LOX protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||tris glycine with 0.15M NaCl|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:250|
|Western Blot (WB)||0.5-4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-16953 is specific for the ~58 kDa LOX protein.
This is a highly glycosylated protein and therefore, other bands may be seen at ~32 kDa (non-glycosylated form) 50 kDa and 58 kDa (glycosylated forms).
Suggested positive control: human and mouse kidney lysate, antigen standard for LOX (transient overexpression lysate), human kidney protein, mouse kidney protein.
Lysyl oxidase (LOX), a copper-containing amine oxidase, belongs to a heterogeneous family of enzymes that oxidize primary amine substrates to reactive aldehydes. It plays a vital role in the formation and repair of the extra cellular matrix. As well, LOX is a multifunctional enzyme having diverse biological functions such as developmental regulation, tumor suppression, cell motility and cellular senescence. The secreted form of LOX is responsible for the invasive properties of hypoxic human cancer cells. Thus, it is essential for hypoxia-induced metastasis and is a good therapeutic target for preventing and treating metastases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.