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|Tested species reactivity||Human, Mouse, Non-human primate, Pig, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide made to an internal region of mouse LOX propeptide (residues 78-115). [UniProt# P28301]|
|Purification||Antigen affinity chromatography|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||0.5-1.0 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:250|
|Western Blot (WB)||1:500-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This is a highly glycosylated protein and can exhibit bands at ~32 kDa, ~50 kDa and ~58 kDa representing the mature (secreted) form, the pro form and the glycosylated forms, respectively.
Suggested positive control: MC3T3-E1 lysate.
Lysyl oxidase (LOX) is an extracellular enzyme that is critical for the normal biosynthesis of collagens and elastin. LOX is synthesized as a 50 kDa precursor, secreted into the extracellular matrix and subsequently proteolytically processed to a 30 kDa active enzyme and an 18 kDa propeptide. LOX also reverts ras-mediated transformation and its expression is down-regulated in human cancers. Most recently, the lysyl oxidase pro-peptide (LOPP) has been associated with tumor suppression, as it inhibits ras-dependent cellular transformation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Lysyl oxidase; Lysyl oxidase (an H-rev gene with its expression down-regulated in HRAS-transformed rat 208F fibroblasts); Lysyl oxidase propeptide; Protein-lysine 6-oxidase; Ras excision protein; ras recision
AI893619; H-rev142; LOX; Rrg; Rrg1; TSC-160