Western blot of M Cadherin in whole cell lysates from COS-7 (lane 1) and human M-cadherin overexpressed in COS-7 cells (lane 2). Samples were separated on SDS-PAGE, transferred to PVDF membrane, and probed using a M Cadherin monoclonal antibody (Product # MA1-91683) at a dilution of 0.5µg/ml for 1 hour at room temperature, followed by ECL detection at an exposure time of 30 seconds.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant full length protein - the extracellular domain of human M-Cadherin|
|Storage buffer||PBS with PEG, sucrose|
|Contains||0.1% sodium azide|
|Storage Conditions||-80° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.05 µg/ml|
|Immunocytochemistry (ICC)||5 ug/ml|
|Immunofluorescence (IF)||5 µg/ml|
|Western Blot (WB)||0.5-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-91683 detects M Cadherin from human samples.
MA1-91683 has been successfully used in immunofluorescence, Western blot applications.
The MA1-91683 immunogen is: Recombinant full length protein - the extracellular domain of human M-Cadherin
This gene encodes a type I integral membrane glycoprotein. The encoded protein acts as a receptor and binds to both soluble and immobilized hyaluronan. This protein may function in lymphatic hyaluronan transport and have a role in tumor metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.