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Flow cytometry analysis of M13 Phage coat protein in Colo320 cells using a M13 Phage coat protein monoclonal antibody (Product # MA1-34468) at a dilution of 10µg/ml (filled profile) compared to an irrelevant phage antibody (open profile). Cells were incubated overnight with 1012 phage particles and secondary detection was performed using a FITC-conjugated rabbit anti-mouse IgG secondary antibody.
|Tested species reactivity||Virus|
|Published species reactivity||Virus|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Full length M13 phage coat protein.|
|Contains||0.08% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Flow Cytometry (Flow)||1 µg for 10^6 cells|
|Immunohistochemistry (IHC)||Assay dependent|
|Western Blot (WB)||1-5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
MA1-34468 detects M13 from many samples.
MA1-34468 has been successfully used in flow cytometry, immunohistochemistry, Western blot and ELISA applications.
The MA1-34468 immunogen is full length M13 phage coat protein.
Store at 4°C short term; for long term storage, store at -20°C, avoiding freeze/thaw cycles.
M13 antibody [E1] has numerous uses in screening phage display libraries, especially the screening of single chain antibody libraries.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Generation of recombinant guinea pig antibody fragments to the human GABAC receptor.
MA1-34468 was used in immunocytochemistry to isolate antibodies to the human GABA(C) receptor
|Memic A,Volgina VV,Gussin HA,Pepperberg DR,Kay BK||Journal of immunological methods (368:36)||2011|