MA1-06510 detects plakophilin-3 in human, mouse, canine, and porcine samples.
MA1-06510 has sucessfully been used in Western blotting, immunoprecipitation, immunocytochemistry, immunohistochemistry (frozen) and immunohistochemistry (methanol-fixed cells) procedures. By Western blot it detects a ~87 kDa band representing pakophilin-3.
The MA1-06510 immunogen is synthetic peptide corresponding to residues 779-793 of human plakophilin-3 coupled to KLH.
Plakophilin-3 is an Armadillo-like protein present in nuclei and desmosomes of epithelial cells. The expression pattern of this protein seems to be largely restricted to epithelial cell types. Plakophilin-3 can be detected along cell borders in a punctuate
staining pattern typical for desmosomal proteins. In addition to the desmosomal immunolocalisation, immunostaining was observed as bright nuclear speckles. Thus, like plakophilin-1 and-2, plakophilin-3 displays a dual intracellular localisation in the desmosomal plaque and in the cell nucleus, and therefore is probably involved in signal transduction pathways between the plasma membrane and the nucleus. The human protein has a predicted molecular mass of ~87 kD.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: plakophilin 3b; Plakophilin-3
Gene Aliases: 2310056L12Rik; PKP3