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          • Primary Antibodies ›
          • FGF2 Antibodies

          Invitrogen

          FGF2 Monoclonal Antibody (F-343)

          Advanced Verification
          View all (40) FGF2 antibodies

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          Cite FGF2 Monoclonal Antibody (F-343)

          Additional Information:
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          • Antibody Testing Data (3)
          • Advanced Verification (2)
          FGF2 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          FGF2 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 5

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          FGF2 Antibody (MA1-21363) in ICC/IF

          Immunofluorescence analysis of FGF2 was performed using 80% confluent log phase BJ and T-47D control and PTI treated (1X for 4 hours) cells. The cells were fixed with 4% paraformaldehyde for 10 mins, permeabilized with 0.1% Triton™ X-100 for 10 mins, and blocked with 2% BSA for 45 mins at room temperature. The cells were labeled with FGF2 Monoclonal Antibody (F-343) (Product # MA1-21363) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          FGF2 Antibody in Immunocytochemistry (ICC/IF)
          FGF2 Antibody in Western Blot (WB)
          FGF2 Antibody in Western Blot (WB)
          FGF2 Antibody
          FGF2 Antibody
          FGF2 Monoclonal Antibody (F-343)

          Product Details

          MA1-21363

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1250
          -

          Immunocytochemistry (ICC/IF)

          1:100
          -

          ELISA (ELISA)

          Assay-dependent
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          F-343

          Immunogen

          Full length native protein (purified) (Human).
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Protein G

          Storage buffer

          PBS, pH 7.2

          Contains

          no preservative

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_558914

          Product Specific Information

          MA1-21363 detects FGF Basic in Human samples.

          Target Information

          FGF2 (FGFb, fibroblast growth factor basic) belongs to the fibroblast growth factor (FGF) family, and interacts with high-affinity transmembrane receptors to influence cell proliferation and tissue neovascularization. FGF2 exists as five isoforms with distinct intracellular localizations and functions. The 18 kDa isoform is predominantly cytosolic and acts through cell surface receptors, whereas the 22, 22.5, 24 and 34 kDa isoforms are nuclear and may signal independent of transmembrane receptor pathways. In humans, the gene is located on the q arm of chromosome 4. FGF2 has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for FGF2 contains multiple polyadenylation sites, and is alternatively translated from non-AUG and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. Diseases associated with FGF2 dysfunction include Kaposi Sarcoma and corneal neovascularization.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: 18 kd basic fibroblast growth factor; 21 kd basic fibroblast growth factor (ctg start codon; put.); putative; Basic fibroblast growth factor; basic fibroblast growth factor (ctg start codon); putative; basic fibroblast growth factor bFGF; bFGF; FGF; fgf basic; FGF-2; FGF-b; FGF2; Fibroblast growth factor; Fibroblast growth factor 2; fibroblast growth factor 2 (basic); Fibroblast growth factor basic; H-FGF-b-147; H-FGF-b-154; HBGF-2; Heparin-binding growth factor 2; M-FGF-b; prostatropin

          View more View less

          Gene Aliases: BFGF; FGF-2; FGF2; FGFB; HBGF-2

          View more View less

          UniProt ID: (Human) P09038

          View more View less

          Entrez Gene ID: (Human) 2247

          View more View less

          Function(s)
          fibroblast growth factor receptor binding cytokine activity integrin binding protein binding growth factor activity heparin binding chemokine binding chemoattractant activity identical protein binding histone H3K9me2/3 reader activity receptor-receptor interaction
          Process(es)
          angiogenesis osteoblast differentiation branching involved in ureteric bud morphogenesis organ induction endothelial cell proliferation positive regulation of endothelial cell proliferation cell migration involved in sprouting angiogenesis positive regulation of neuroblast proliferation chromatin organization transcription from RNA polymerase II promoter chemotaxis signal transduction Ras protein signal transduction nervous system development neuroblast proliferation cell proliferation positive regulation of cell proliferation negative regulation of cell proliferation fibroblast growth factor receptor signaling pathway embryo development ending in birth or egg hatching organ morphogenesis glial cell differentiation positive regulation of endothelial cell migration positive regulation of gene expression negative regulation of gene expression negative regulation of fibroblast migration growth factor dependent regulation of skeletal muscle satellite cell proliferation substantia nigra development cerebellar granule cell precursor proliferation positive regulation of cerebellar granule cell precursor proliferation neurogenesis cell differentiation hyaluronan catabolic process lung development regulation of cell migration paracrine signaling negative regulation of fibroblast growth factor receptor signaling pathway wound healing auditory receptor cell differentiation positive regulation of cell fate specification positive regulation of MAP kinase activity positive regulation of MAPK cascade protein kinase B signaling positive regulation of blood vessel endothelial cell migration negative regulation of blood vessel endothelial cell migration endothelial cell migration response to ethanol positive regulation of auditory receptor cell differentiation positive regulation of osteoblast differentiation regulation of angiogenesis positive regulation of angiogenesis positive regulation of transcription from RNA polymerase II promoter regulation of retinal cell programmed cell death behavioral response to ethanol embryonic morphogenesis positive regulation of smooth muscle cell proliferation response to axon injury stem cell differentiation stem cell development epithelial cell proliferation positive regulation of epithelial cell proliferation positive chemotaxis release of sequestered calcium ion into cytosol regulation of cell cycle positive regulation of cell division positive regulation of protein kinase B signaling positive regulation of cardiac muscle cell proliferation canonical Wnt signaling pathway corticotropin hormone secreting cell differentiation thyroid-stimulating hormone-secreting cell differentiation neuroepithelial cell differentiation chondroblast differentiation mammary gland epithelial cell differentiation angiogenesis involved in coronary vascular morphogenesis negative regulation of wound healing ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade cellular response to mechanical stimulus stem cell proliferation regulation of cell migration involved in sprouting angiogenesis positive regulation of cell migration involved in sprouting angiogenesis positive regulation of canonical Wnt signaling pathway positive regulation of lens fiber cell differentiation positive regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of neuroepithelial cell differentiation negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis positive regulation of sprouting angiogenesis response to wortmannin positive regulation of vascular smooth muscle cell proliferation lymphatic endothelial cell migration positive regulation of epithelial tube formation regulation of morphogenesis of an epithelium positive regulation of blood vessel branching positive regulation of vascular endothelial cell proliferation regulation of endothelial cell chemotaxis to fibroblast growth factor positive regulation of endothelial cell chemotaxis to fibroblast growth factor positive regulation of DNA biosynthetic process negative regulation of stem cell proliferation positive regulation of stem cell proliferation positive regulation of stem cell differentiation positive regulation of endothelial cell chemotaxis
          It has to be done as per old AB suggested Products section.

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