|Immunohistochemistry (Paraffin) (IHC (P))||Assay-dependent|
|Western Blot (WB)||1-5 µg/mL|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Amino acids 1-153 of human ER-ß expressed in E. coli.|
|Storage buffer||PBS, pH 7.4|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA1-23219 detects Estrogen Receptor Beta in Human samples.
MA1-23219 has been successfully used in Western Blot procedures.
MA1-23219 immunogen corresponds to Amino acids 1-153 of human ER-ß expressed in E. coli..
Estrogen Receptor beta (ER beta, NR3A2) protein is approximately 55kD, though multiple isoforms ranging from 36-59kD have been described. Human ER beta was initially cloned and characterized from testis. Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Estrogen receptor beta (ER beta) binds estrogens with an affinity similar to that of estrogen receptor alpha. Some ER beta isoforms dominantly inhibit the activity of estrogen receptor alpha in reproductive tissues. ER beta is found widely in many tissues throughout the body and can act as a potent tumor suppressor, playing a crucial role in many cancer types such as prostate cancer, autism spectrum disorder, medullary thyroid carcinoma, De Quervain Disease and risk of cardiovascular disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ER Beta; ER-beta; ESR-Beta; ESR2; estrogen receptor 2 (ER beta); Estrogen receptor beta; estrogen receptor beta 4; Nuclear receptor subfamily 3 group A member 2
Gene Aliases: ER-BETA; Erb; ESR-BETA; ESR2; ESRB; ESTRB; NR3A2
UniProt ID: (Human) Q92731
Entrez Gene ID: (Human) 2100
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.