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Invitrogen
Human IgG2a Isotype Control (LO-DNP-16)
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Product Details
MA1-90900
Applications
Tested Dilution
Immunohistochemistry (Frozen) (IHC (F))
Assay-dependent
Flow Cytometry (Flow)
10 µL for 10^6 cells in 100 µL
ELISA (ELISA)
Assay-dependent
Product Specifications
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Isotype Control
Clone
LO-DNP-16
Immunogen
Human lymphocytes
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
MA1-90900 detects IgG2a from human samples.
MA1-90900 has been successfully used in flow cytometry, ELISA, immunohistochemistry (frozen tissue) applications.
The MA1-90900 immunogen is: Human lymphocytes
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.
Target Information
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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