This Antibody was verified by Cell Treatment to ensure that the antibody binds to the antigen stated. View Details
This antibody detects Flag tag from various samples.
similar epitope as the FLAG tag from Sigma
MA1-91878 has been successfully used in immunoprecipitation, Western blot and immunofluorescence applications.
The MA1-91878 immunogen is synthetic peptide (DYKDDDDK) coupled to KLH.
MA1-91878 may be stored at 4°C short term. Add 0.05% sodium azide if desired. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
DYKDDDDK Tag (FLAG-tag, FLAG octapeptide) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DDDDK; DDDDK epitope tag; DYKDDDDK epitope tag; ECS epitope tag; ECS tag; Enterokinase Cleavage Site epitope tag; Enterokinase Cleavage Site tag; FLAG; FLAG Tag