Invitrogen

MYF5 Monoclonal Antibody (OTI2G5)

View all (16) MYF5 antibodies

Questions & Answers

Questions & Answers

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MYF5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human endometrium tissue. To expose target proteins, 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a MYF5 monoclonal antibody (Product # MA5-26654).

MYF5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human colon tissue. To expose target proteins, 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a MYF5 monoclonal antibody (Product # MA5-26654).

MYF5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded carcinoma of human prostate tissue. To expose target proteins, 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a MYF5 monoclonal antibody (Product # MA5-26654).

MYF5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded carcinoma of human bladder tissue. To expose target proteins, 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a MYF5 monoclonal antibody (Product # MA5-26654).

MYF5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded carcinoma of human thyroid tissue. To expose target proteins, 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a MYF5 monoclonal antibody (Product # MA5-26654).

MYF5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded carcinoma of human kidney tissue. To expose target proteins, 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a MYF5 monoclonal antibody (Product # MA5-26654).

MYF5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded carcinoma of human lung tissue. To expose target proteins, 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a MYF5 monoclonal antibody (Product # MA5-26654).

MYF5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded human pancreas tissue. To expose target proteins, 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a MYF5 monoclonal antibody (Product # MA5-26654).

MYF5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded human thyroid tissue. To expose target proteins, 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a MYF5 monoclonal antibody (Product # MA5-26654).

MYF5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded human tonsil tissue. To expose target proteins, 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a MYF5 monoclonal antibody (Product # MA5-26654).

MYF5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded human breast tissue. To expose target proteins, 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a MYF5 monoclonal antibody (Product # MA5-26654).

MYF5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded human ovary tissue. To expose target proteins, 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a MYF5 monoclonal antibody (Product # MA5-26654).

MYF5 Antibody in Western Blot (WB) — Western blot analysis of MYF5 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with MYF5 (Product # MA5-26654) monoclonal antibody.

Product Details

MA5-26654

Applications	Tested Dilution	Publications
Western Blot (WB)	1:2,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:150	-

Product Specifications
Species Reactivity	Human
Published species	Mouse
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	OTI2G5
Immunogen	Full length human recombinant protein of MYF5 produced in E.coli View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS, pH 7.3, with 1% BSA, 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2724766

Target Information

MYF5 is a member of the myogenic basic helix-loop-helix family of transcription factors, which can activate the muscle differentiation program.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A newly identified lncRNA MAR1 acts as a miR-487b sponge to promote skeletal muscle differentiation and regeneration.

Journal of cachexia, sarcopenia and muscle

Zhang ZK,Li J,Guan D,Liang C,Zhuo Z,Liu J,Lu A,Zhang G,Zhang BT

MA5-26654 was used in Western Blotting to show lncRNA MAR1 acts as a miR-487b sponge to regulate Wnt5a protein, promoting muscle differentiation and regeneration.

Fri Jun 01 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: bHLHc2; Class C basic helix-loop-helix protein 2; Myf-5; Myogenic factor 5

Gene Aliases: BHLHC2; MYF5

UniProt ID: (Human) P13349

Entrez Gene ID: (Human) 4617

Molecular Function: basic helix-loop-helix transcription factor

cartilage condensation ossification somitogenesis regulation of cell-matrix adhesion regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter muscle organ development skeletal muscle tissue development extracellular matrix organization skeletal muscle cell differentiation muscle cell fate commitment camera-type eye development positive regulation of myoblast differentiation positive regulation of transcription from RNA polymerase II promoter embryonic skeletal system morphogenesis positive regulation of skeletal muscle fiber development positive regulation of muscle cell differentiation muscle tissue morphogenesis positive regulation of myoblast fusion

Process(es)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein binding protein heterodimerization activity E-box binding