|Tested species reactivity||Human, Mouse|
|Host / Isotype||Goat / IgG|
|Immunogen||Synthetic peptide corresponding to amino acids CHTMPIADPQPLD from C-Terminus of Human MADH4|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.5-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-31458 detects MADH4 from human and mouse samples. Expected to cross react with rat samples due to sequence homology.
PA1-31458 has been successfully used in Western blot applications. A positive control for this product is NIH-3T3 cell lysate.
The PA1-31458 immunogen is synthetic peptide - amino acids CHTMPIADPQPLD from C-Terminus of Human MADH4.
Smad4 is a common mediator of signal transduction by the TGF-beta (transforming growth factor) superfamily; it may act as a tumor suppressor.
IP-MS enrichment of SMAD4 (LFQ intensity): SMAD4 was enriched 726-fold from HeLa lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Part No. 90409) and SMAD4 antibody (Part No. PA1-31458). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.