This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. View Details
Description: The monoclonal antibody 50 recognizes human MALT1 (Mucosal Associated Lymphoid Tissue lymphoma translocation gene 1). MALT1 is a 91 kDa paracaspase that contains a death domain, two Ig-like domains and a C terminal caspase-like domain. Like other caspases, it cleaves substrates after an arginine residue. Expression is found in the cytoplasm of B and T cells. Within B cell follicles, MALT1 expression is highly expressed in centroblasts, followed by centrocytes and weakly expressed in the mantle zone. The interaction of MALT1 and Bcl10 occurs through Ig-like domains resulting in oligomerization and activation of the caspase-like domain. This also directly results in NFkappa B activation thereby playing a role in B cell maturation and activation.
Rearrangements of the MALT1 gene found in t(11;18)(q21;q21) and t(14;18)(q32;q21) are the most frequent structural chromosomal abnormalities in MALT lymphomas. These translocations lead to fusions of API2(BIRC3)-MALT1 and IGH-MALT1 respectively. An additional translocation between MALT1 and MAP4 has also been identified in DLBCL (diffuse large B- cell lymphoma).
The antibody 50 reacts with the C terminal domain of MALT1, which allows for the detection of endogenous MALT1 as well as both identified fusion proteins resulting from translocation events.
Applications Reported: This 50 antibody has been reported for use in immunoblotting (WB) and immunohistochemical staining of formalin-fixed, paraffin embedded tissue sections.
Applications Tested: This 50 antibody has been tested by immunohistochemistry on formalin-fixed paraffin-embedded (FFPE) human tonsil tissue. Antigen retrieval was performed using 10mM citrate buffer at pH 6.0. For immunohistochemistry, this antibody can be used at less than or equal to 10 µg/mL. This 50 antibody has been tested by western blotting under reducing conditions (at 5 µ/mL) on Jurkat cells. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
MALT1/Paracaspase (MALT1) was independently identified as a member of the human paracaspase family and an interacting partner of B-cell lymphoma (Uren et al., 2000). MALT1 is a caspase-like protein that contains an N-terminal death domain, two Ig-like domains, and a C-terminal caspase-like domain. It binds to Bcl10 through its Ig-like domains and cooperates with Bcl10 to activate NF-kappaB.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: caspase-like protein; DKFZp434L132; MALT lymphoma-associated translocation; malt-1; MALT1 protease; mucosa associated lymphoid tissue lymphoma translocation gene 1; Mucosa-associated lymphoid tissue lymphoma translocation protein 1; Paracaspase; paracaspase 1; paracaspase-1
Gene Aliases: IMD12; MALT1; MLT; MLT1; PCASP1
UniProt ID: (Human) Q9UDY8
Entrez Gene ID: (Human) 10892