Invitrogen

MAN1 Monoclonal Antibody (OTI3H1)

View all (18) MAN1 antibodies

Questions & Answers

Questions & Answers

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MAN1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of LEMD3 in COS7 cells. Cells were transfected with a plasmid overexpressing LEMD3 and probed with a LEMD3 monoclonal antibody (Product # MA5-25020).

MAN1 Antibody in Western Blot (WB) — Immunoprecipitation was performed on HEK293T cells overexpressing LEMD3. Antigen-antibody complexes were formed by incubating the sample with 2 µg of LEMD3 monoclonal antibody (Product # MA5-25020) antibody and 0.1 mg of goat anti-mouse conjugated magnetic beads overnight. The sample was washed extensively and probed with rabbit anti-DDK polyclonal antibody.

MAN1 Antibody in Western Blot (WB) — Western blot analysis of LEMD3 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with LEMD3 (Product # MA5-25020) monoclonal antibody.

MAN1 Antibody in Western Blot (WB) — Western blot analysis of LEMD3 in HepG2, HeLa, HT29, A549, COS7, Jurkat, MDCK, PC12, MCF7 cells using 35 µg per lane. Samples were probed with LEMD3 (Product # MA5-25020) monoclonal antibody.

Product Details

MA5-25020

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500	-
Immunocytochemistry (ICC/IF)	1:100	-
Flow Cytometry (Flow)	1:100	-
Immunoprecipitation (IP)	2-4 µg/mL	-

Product Specifications
Species Reactivity	Dog, Human, Non-human primate, Rat
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	OTI3H1
Immunogen	Full length human recombinant protein of LEMD3 produced in HEK293T cell View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS, pH 7.3, with 1% BSA, 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2723351

Target Information

Can function as a specific repressor of TGF-beta, activin, and BMP signaling through its interaction with the R-SMAD proteins. Antagonizes TGF-beta-induced cell proliferation arrest.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Inner nuclear membrane protein Man1; integral inner nuclear membrane protein; LEM domain-containing protein 3

Gene Aliases: LEMD3; MAN1; RGD1561001

UniProt ID: (Human) Q9Y2U8

Entrez Gene ID: (Dog) 608380, (Human) 23592, (Rat) 680066

angiogenesis blood vessel endothelial cell migration involved in intussusceptive angiogenesis nucleus organization negative regulation of transforming growth factor beta receptor signaling pathway negative regulation of BMP signaling pathway negative regulation of activin receptor signaling pathway skeletal muscle cell differentiation regulation of cell cycle regulation of extracellular matrix organization

Process(es)

nucleotide binding DNA binding protein binding