Invitrogen

MAN1 Monoclonal Antibody (OTI3H1)

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MAN1 Antibody in Western Blot (WB) — Immunoprecipitation was performed on HEK293T cells overexpressing LEMD3. Antigen-antibody complexes were formed by incubating the sample with 2 µg of LEMD3 monoclonal antibody (Product # MA5-25020) antibody and 0.1 mg of goat anti-mouse conjugated magnetic beads overnight. The sample was washed extensively and probed with rabbit anti-DDK polyclonal antibody.

MAN1 Antibody in Immunocytochemistry (ICC) — Immunofluorescent analysis of LEMD3 in COS7 cells. Cells were transfected with a plasmid overexpressing LEMD3 and probed with a LEMD3 monoclonal antibody (Product # MA5-25020).

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

Immunocytochemistry (ICC)

1:100

Immunofluorescence (IF)

1:100

Flow Cytometry (Flow)

1:100

Immunoprecipitation (IP)

2-4 µg/mL

Product Specifications

Species Reactivity

Dog, Human, Non-human primate, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI3H1

Immunogen

Full length human recombinant protein of LEMD3 produced in HEK293T cell

Conjugate

Unconjugated

Form

Liquid

Concentration

0.95 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2723351

Target Information

Can function as a specific repressor of TGF-beta, activin, and BMP signaling through its interaction with the R-SMAD proteins. Antagonizes TGF-beta-induced cell proliferation arrest.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Inner nuclear membrane protein Man1; integral inner nuclear membrane protein; LEM domain-containing protein 3

Gene Aliases: LEMD3; MAN1; RGD1561001

UniProt ID: (Human) Q9Y2U8

Entrez Gene ID: (Dog) 608380, (Human) 23592, (Rat) 680066

Function(s)

angiogenesis blood vessel endothelial cell migration involved in intussusceptive angiogenesis nucleus organization negative regulation of transforming growth factor beta receptor signaling pathway negative regulation of BMP signaling pathway negative regulation of activin receptor signaling pathway skeletal muscle cell differentiation regulation of cell cycle regulation of extracellular matrix organization

Process(es)

nucleotide binding DNA binding protein binding

MAN1 Monoclonal Antibody (OTI3H1)

Product Details

Target Information

References (0)

Bioinformatics

Material safety data sheets (MSDS)

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