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Immunofluorescence analysis of neurons using anti-MAP2 antibody (MA5-12823). GABAergic precursor cells derived from H9 embryonic stem cells were differentiated for 7 days and stained with antibodies against MAP2 (Cat. No. MA5-12823 at 1:200) followed by Alexa Fluor 488 goat anti-rabbit (Cat. No. A21206, green). Nuclear DNA was stained with DAPI (blue) in the merged image (right panel).
|Tested species reactivity||Human|
|Published species reactivity||Rat, Primate, Human, Mouse, Clawed frog|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Bovine brain microtubule associated protein 2|
|Storage buffer||tissue culture supernatant|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-200|
|Western Blot (WB)||1:20-50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is specific to MAP2a and MAP2b, and will not cross-react with MAP1, MAP5, tubulin or tau. This clone reacts with dendrites and cell bodies of neurons but not with the neuronal processes. It does not react with the low MW form (70 kDa, MAP2c). The epitope has been mapped to aa 997-1332.
The molecular weight of the doublet is ~280 kDa.
For IHC staining, formalin-fixed tissues must be boiled in a 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at room temperature for 20 min. Recommened positive controls include human glioblastoma T98G cells or brain tissue.
Microtubule Associated Protein 2 exists in two high molecular weight forms (2a & 2b) and a low molecular weight form. The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene. Ab-2 is highly specific to MAP2a,b and shows no cross-reaction with MAP1, MAP5, tubulin, or tau. Ab-2 reacts with dendrites and cell bodies of neurons but not with the neuronal processes. It does not react with the low molecular weight (70kDa) form (2c) of MAP2. It is useful in studies of neuron structure in normal and malignant brain tissue as well as in degenerative diseases such as Alzheimer's.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Precocious and delayed neocortical synaptogenesis in fetal holoprosencephaly.
MA5-12823 was used in immunohistochemistry to study neocortical synaptogenesis in fetal holoprosencephaly by monitoring synaptophysin expression
|Sarnat HB,Flores-Sarnat L||Clinical neuropathology (32:255)||2013|
Hemimegalencephaly: foetal tauopathy with mTOR hyperactivation and neuronal lipidosis.
MA5-12823 was used in immunohistochemistry to report on three cases of hemimegalencephaly
|Sarnat H,Flores-Sarnat L,Crino P,Hader W,Bello-Espinosa L||Folia neuropathologica / Association of Polish Neuropathologists and Medical Research Centre, Polish Academy of Sciences (50:330)||2013|
Mesenchymal stem cells improve the healing of ischemic colonic anastomoses (experimental study).
MA5-12823 was used in immunohistochemistry to study the role of allogenic mesenchymal stem cell transplantation in improved healing of ischemic colon anastomosis
|Adas G,Arikan S,Karatepe O,Kemik O,Ayhan S,Karaoz E,Kamali G,Eryasar B,Ustek D||Langenbeck's archives of surgery / Deutsche Gesellschaft fu¿r Chirurgie (396:115)||2011|
Optimizing orthotopic cell transplantation in the mouse adrenal gland.
MA5-12823 was used in immunohistochemistry to establish and evaluate a novel orthotopic cell transplantation model
|Cardoso CC,Bornstein SR,Hornsby PJ||Cell transplantation (19:565)||2010|
eIF2alpha Phosphorylation-dependent translation in CA1 pyramidal cells impairs hippocampal memory consolidation without affecting general translation.
MA5-12823 was used in immunohistochemistry to identify translation mechanism most important for memory in mammalian brains
|Jiang Z,Belforte JE,Lu Y,Yabe Y,Pickel J,Smith CB,Je HS,Lu B,Nakazawa K||The Journal of neuroscience : the official journal of the Society for Neuroscience (30:2582)||2010|
Characterization of neogenin-expressing neural progenitor populations and migrating neuroblasts in the embryonic mouse forebrain.
MA5-12823 was used in immunohistochemistry to characterize neogenin-expressing neural progenitor populations and migrating neuroblasts in the embryonic mouse forebrain
|Fitzgerald DP,Cole SJ,Hammond A,Seaman C,Cooper HM||Neuroscience (142:703)||2006|
Architectural (Type IA) focal cortical dysplasia and parvalbumin immunostaining in temporal lobe epilepsy.
MA5-12823 was used in immunohistochemistry to investigate the association between type IA cortical dysplasia and epileptogenicity
|Garbelli R,Meroni A,Magnaghi G,Beolchi MS,Ferrario A,Tassi L,Bramerio M,Spreafico R||Epilepsia (47:1074)||2006|
ABCA1 influences neuroinflammation and neuronal death.
MA5-12823 was used in immunohistochemistry - paraffin section to elucidate the cell-type specific role of ABCA in neuroinflammation in vivo.
|Karasinska JM,de Haan W,Franciosi S,Ruddle P,Fan J,Kruit JK,Stukas S,Lütjohann D,Gutmann DH,Wellington CL,Hayden MR||Neurobiology of disease (54:445)||2013|
Characterization of glioma stem cells through multiple stem cell markers and their specific sensitization to double-strand break-inducing agents by pharmacological inhibition of ataxia telangiectasia mutated protein.
MA5-12823 was used in immunocytochemistry to study the ability of ataxia telangiectasia mutated protein inhibitors to sensitize tumor-driving glioma stem cells to treatments that induce DNA double-strand breaks
|Raso A,Vecchio D,Cappelli E,Ropolo M,Poggi A,Nozza P,Biassoni R,Mascelli S,Capra V,Kalfas F,Severi P,Frosina G||Brain pathology (Zurich, Switzerland) (22:677)||2012|
CRH-R1 and CRH-R2 differentially modulate dendritic outgrowth of hippocampal neurons.
MA5-12823 was used in immunocytochemistry to study the distinct roles of CRH-R1 and CRH-R2 in regulating the outgrowth of cultured hippocampal neurons
|Sheng H,Xu Y,Chen Y,Zhang Y,Xu X,He C,Ni X||Endocrine (41:458)||2012|
Phenotypic characteristics of hybrid cells generated by transferring neuronal nuclei into bone marrow stromal cell cytoplasts.
MA5-12823 was used in immunocytochemistry to characterize the structural and functional phenotype of the hybrid cells with neuronal nuclei and bone marrow stromal cell cytoplasts
|Zhou Z,Xu Y,Zhong Q,Zheng J||Brain research bulletin (87:303)||2012|
Neural stem cells express non-neural markers during embryoid body coculture.
MA5-12823 was used in immunocytochemistry to study the expresssion of non-neural markers by neural stem cells during embryoid body co-culture
|Denham M,Huynh T,Dottori M,Allen G,Trounson A,Mollard R||Stem cells (Dayton, Ohio) (24:918)||2006|
MKLP1 requires specific domains for its dendritic targeting.
MA5-12823 was used in immunocytochemistry to study the mechanism for the targeted transport of mitotic kinesin-like protein 1
|Xu X,He C,Zhang Z,Chen Y||Journal of cell science (119:452)||2006|
The carboxy-terminal tail region of human Cav2.1 (P/Q-type) channel is not an essential determinant for its subcellular localization in cultured neurones.
MA5-12823 was used in immunocytochemistry to study the role of carboxy-terminal tail region of human Cav2.1 in its subcellular localization
|Hu Q,Saegusa H,Hayashi Y,Tanabe T||Genes to cells : devoted to molecular & cellular mechanisms (10:87)||2005|
BetaAPP and furin mRNA concentrates in immature senile plaques in the brain of Alzheimer patients.
MA5-12823 was used in immunocytochemistry to study the accumulation of BetaAPP and furin mRNA in immature senile plaques in the brain of Alzheimer patients
|Marcinkiewicz M||Journal of neuropathology and experimental neurology (61:815)||2002|
|Clawed frog||Not Cited||
Suppression of sprouting: An early function of NMDA receptors in the absence of AMPA/kainate receptor activity.
MA5-12823 was used in immunocytochemistry to investigate the influence of NMDAR on dendritic sprouting and its mechanism
|Lin SY,Constantine-Paton M||The Journal of neuroscience : the official journal of the Society for Neuroscience (18:3725)||1998|
MAP2; MAP2A; MAP2B; MAP2C