Immunofluorescent analysis of microtubule-associated protein 2 (MAP2, green) in cultured primary cortical neurons at different developmental stages (day 1 and day 14). Primary cortical neurons were isolated and cultured using the Primary Neuron Isolation Kit (Product # 88280). Neurons were fixed with 4% paraformaldehyde, permeablilized with 0.1% Triton X-100 in HBSS for 10 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were probed with a MAP2 monoclonal antibody, clone AP18 (Product # MA5-12826) at a dilution of 1:500 for 2 hours at room temperature (or overnight at 4°C), washed with HBSS, and incubated with a DyLight 488 goat anti-mouse IgG (Product # 35502) secondary antibody at a dilution of 1:500 for 1 hour at room temperature (panels A and B). In panel C, cells were also probed with a glial fibrillary acidic protein (GFAP) polyclonal antibody at a dilution of 1:500 for 2 hours at room temperature (or overnight at 4°C), washed with HBSS, and incubated with a DyLight 680 goat anti-rabbit IgG (Product # 35568) secondary antibody at a dilution of 1:500 for 1 hour at room temperature. Images were taken at 20X magnification on a Carl Zeiss microscope (AxioVision Rel. 4.7).
|Tested species reactivity||Avian, Bovine, Human, Mouse, Rat, Xenopus|
|Published species reactivity||Rat, Human, Mouse, Xenopus, Guinea pig|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Bovine brain microtubule associated protein 2|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1-2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12826 has been successfully used in immunofluorescence analysis of Map2 in human glutamate neurons derived from iPSCs.
Microtubule Associated Protein 2 exists in two high molecular weight forms (MAP2a and MAP2b) and a low molecular weight form (MAP2c). The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dendrites, implicating a role in determining and stabilizing dendritic shape during neuron development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
NRP1-mediated Sema3A signals coordinate laminar formation in the developing chick optic tectum.
MA5-12826 was used in immunocytochemistry to study Neuropilin 1-mediated Sema3A signaling in the optic tectum
|Watanabe Y,Sakuma C,Yaginuma H||Development (Cambridge, England) (141:3572)||2014|
Semaphorin 3A induces CaV2.3 channel-dependent conversion of axons to dendrites.
MA5-12826 was used in immunocytochemistry to determine the mechanism of Ca-dependent neurite polarization
|Nishiyama M,Togashi K,von Schimmelmann MJ,Lim CS,Maeda S,Yamashita N,Goshima Y,Ishii S,Hong K||Nature cell biology (13:676)||2011|
Successful isolation and long-term establishment of a cell line with stem cell-like features from an anaplastic medulloblastoma.
MA5-12826 was used in immunocytochemistry to isolate and establish a cell line with stem cell-like features from an anaplastic medulloblastoma
|Raso A,Negri F,Gregorio A,Nozza P,Mascelli S,De Marco P,Merello E,Milanaccio C,Ravegnani M,Cama A,Garrè ML,Capra V||Neuropathology and applied neurobiology (34:306)||2008|
Enzymatic properties and localization of motopsin (PRSS12), a protease whose absence causes mental retardation.
MA5-12826 was used in immunocytochemistry to characterize the central nervous system mosaic protease motopsin
|Mitsui S,Yamaguchi N,Osako Y,Yuri K||Brain research (1136:1)||2007|
Differential localization of ErbB receptor ensembles influences their signaling in hippocampal neurons.
MA5-12826 was used in immunocytochemistry to investigate the influence of ErbB localization on its signaling in hippocampal neurons
|Chen J,Tseng HC,Dichter MA,Zhang H,Greene MI||DNA and cell biology (24:553)||2005|
Fetal human neural progenitors can be the target for tumor transformation.
MA5-12826 was used in immunocytochemistry to study the transforming potential of fetal human neural progenitor cells
|Wang Y,Bai Y,Li X,Hu Q,Lin C,Xiao Z,Liu Y,Xu J,Shen L,Li L||Neuroreport (15:1907)||2004|
Involvement of microglia-neuron interactions in the tumor necrosis factor-alpha release, microglial activation, and neurodegeneration induced by trimethyltin.
MA5-12826 was used in immunocytochemistry to study the pathogenesis of trimethyltin toxicity
|Eskes C,Juillerat-Jeanneret L,Leuba G,Honegger P,Monnet-Tschudi F||Journal of neuroscience research (71:583)||2003|
Suppression of sprouting: An early function of NMDA receptors in the absence of AMPA/kainate receptor activity.
MA5-12826 was used in immunocytochemistry to investigate the influence of NMDAR on dendritic sprouting and its mechanism
|Lin SY,Constantine-Paton M||The Journal of neuroscience : the official journal of the Society for Neuroscience (18:3725)||1998|
AAD-2004 Attenuates Progressive Neuronal Loss in the Brain of Tg-betaCTF99/B6 Mouse Model of Alzheimer Disease.
MA5-12826 was used in immunohistochemistry to study the protective effects of an anti-oxidative/anti-inflammatory small molecule against neuronal loss in a murine Alzheimer's disease model
|Baek IS,Kim TK,Seo JS,Lee KW,Lee YA,Cho J,Gwag BJ,Han PL||Experimental neurobiology (22:31)||2013|
Intracranial extraskeletal myxoid chondrosarcoma : case report and literature review.
MA5-12826 was used in immunohistochemistry to report on a case of intracranial extraskeletal myxoid chondrosarcoma
|Park JH,Kim MJ,Kim CJ,Kim JH||Journal of Korean Neurosurgical Society (52:246)||2012|
Intramedullary papillary ependymoma with choroid plexus differentiation and cerebrospinal fluid dissemination to the brain.
MA5-12826 was used in immunohistochemistry to report a rare clinical case of spinal papillary ependymoma
|Dulai MS,Caccamo DV,Briley AL,Edwards MS,Fisher PG,Lehman NL||Journal of neurosurgery. Pediatrics (5:511)||2010|
Electrophysiological recording of re-aggregating brain cell cultures on multi-electrode arrays to detect acute neurotoxic effects.
MA5-12826 was used in immunohistochemistry to evaluate a brain cell re-aggregating culture method for the study of neurotoxicity
|van Vliet E,Stoppini L,Balestrino M,Eskes C,Griesinger C,Sobanski T,Whelan M,Hartung T,Coecke S||Neurotoxicology (28:1136)||2007|
Translational control of glial glutamate transporter EAAT2 expression.
MA5-12826 was used in immunohistochemistry to investigate post-transcriptional control of glial glutamate transporter EAAT2 mRNA
|Tian G,Lai L,Guo H,Lin Y,Butchbach ME,Chang Y,Lin CL||The Journal of biological chemistry (282:1727)||2007|
Immunolocalization of MAP-2 in routinely formalin-fixed, paraffin-embedded guinea pig brain sections using microwave irradiation: a comparison of different combinations of antibody clones and antigen retrieval buffer solutions.
MA5-12826 was used in immunohistochemistry to study the efficacy of different microwave pre-treatments to restore MAP-2 immunoreactivity in fixed tissue preparations
|Kan RK,Pleva CM,Hamilton TA,Petrali JP||Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada (11:175)||2005|
Involvement of glial cells in the neurotoxicity of parathion and chlorpyrifos.
MA5-12826 was used in immunohistochemistry to investigate the effect of glial cells on the organophosphorus pesticide neurotoxicity
|Zurich MG,Honegger P,Schilter B,Costa LG,Monnet-Tschudi F||Toxicology and applied pharmacology (201:97)||2004|
Altered levels and distribution of microtubule-associated proteins before disease onset in a mouse model of amyotrophic lateral sclerosis.
MA5-12826 was used in western blot to study altered levels and distribution of microtubule-associated proteins in a mouse model of amyotrophic lateral sclerosis
|Farah CA,Nguyen MD,Julien JP,Leclerc N||Journal of neurochemistry (84:77)||2003|
Simultaneous suppression of cdc2 and cdk2 activities induces neuronal differentiation of PC12 cells.
MA5-12826 was used in western blot to study the role of cdc2 and cdk2 in neuronal differentiation
|Dobashi Y,Shoji M,Kitagawa M,Noguchi T,Kameya T||The Journal of biological chemistry (275:12572)||2000|