Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Product Details
CF505876
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
OTI5C7
Immunogen
Full length human recombinant protein of human MAP2K3 produced in HEK293T cell.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
MAP2K3 (MEK3) is a dual-specificity protein kinase which acts in cellular signal transduction pathways, and is necessary for the expression of glucose transporter. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for MEK3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Dual specificity mitogen-activated protein kinase kinase 3; EC 2.7.12.2; MAP kinase kinase 3; MAPK/ERK kinase 3; MAPKK 3; MEK 3; SAPK kinase 2; Stress-activated protein kinase kinase 2
Gene Aliases: MAP2K3; MAPKK3; MEK3; MKK3; PRKMK3; SAPKK-2; SAPKK2; SKK2
UniProt ID: (Human) P46734
Entrez Gene ID: (Dog) 489547, (Human) 5606
MAPK cascade
activation of MAPK activity
inflammatory response
signal transduction
peptidyl-tyrosine phosphorylation
proteolysis in other organism
regulation of cytokine biosynthetic process
positive regulation of protein kinase activity
positive regulation of transcription, DNA-templated
cardiac muscle contraction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support