Western blot analysis of Mip1 in either human heart lysate in the 1) absence and 2) presence of immunizing peptide or in human skeletal muscle lysate in the 3) absence and 4) presence of immunizing peptide using a CD191/CCR1 polyclonal antibody (Product # PA1-41148) at a 0.5 ug/ml concentration.
|Tested species reactivity||Avian, Bovine, Dog, Chicken, Human, Mouse, Rat, Zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide correspoonding to residues N(85) T A Q R L E R L R K E R Q N Q I K(102) of human Mip1.|
|Storage buffer||PBS with 0.2% gelatin|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.5-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: human skeletal muscle and heart protein or cell lysate.
Mip1 is a novel member of a group of highly conserved proteins. Originally identified as a RAS suppressor protein, Mip1 is a human ortholog of S. cerevisiae Sin1. Studies reveal that Mip1 interacts with proteins involved in MAPK signaling including MEKK2 and participates in the regulation of MEKK2 activation and signaling. It also has been found to bind to JNK and might act as a scaffold molecule in the regulation of JNK signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.