|Tested species reactivity||Bovine, Dog, Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues 100-150 of human SAPK interacting protein 1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 0.2% gelatin|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||10 µg/ml|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
SAPK interacting protein 1, a stress-activated protein kinase, is an evolutionarily conserved component of the eukaryotic SAPK pathway belonging to the SIN1 family. There are multiple isoforms with one being a 522-amino acid protein with 2 bipartite nuclear localization signals, a peroxisomal targeting signal, and a PEST motif for rapid protein degradation. SAPK interacting protein 1 forms a complex with inactive and nonphosphorylated MEKK2 and in the process regulates its dimerization and activation. It is also known to maintain CRTC2 complex integrity and regulates Akt phosphorylation and cell signaling. Recent reports suggest that it acts as scaffold molecule in the regulation of signaling by JNK and takes part in RAS signal pathway. It expresses in all tissues examined, with highest levels in skeletal muscle and heart.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.