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Invitrogen
MARCO Monoclonal Antibody (PLK-1)
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Product Details
MA1-40315
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human
Published species
Human
Host/Isotype
Mouse
/ IgG3
Class
Monoclonal
Type
Antibody
Clone
PLK-1
Immunogen
Human alveolar macrophages (recognizes domain V between residues 420 and 431)
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS with 0.1% BSA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Endotoxin: 0.40 EU/mg
Application Notes: IHC (frozen) - tissue sections can be fixed in acetone or 2% paraformaldehyde. Western blot - use non-reducing conditions. Flow - this antibody weakly stains alveolar macrophages by recognizing the extracellular domain of MARCO, with transfected COS cells used as a positive control. Immunoprecipitation - will immunoprecipitate MARCO as a 60 kDa and 50 kDa protein from transfected COS lysates. Functional Assays - blocks human alveolar macrophages by recognizing the extracellular doamin of MARCO.
Recommended positive control: Human alveolar macrophages; Negative control: All other human cells.
Target Information
MARCO, also known as SR-A6 or SCARA2, is a macrophage surface receptor and a member of the class A scavenger receptor family. As a part of the innate antimicrobial immune system, MARCO can bind both Gram-negative and Gram-positive bacteria via an extracellular, C-terminal, scavenger receptor cysteine-rich (SRCR) domain. In addition to short cytoplasmic and transmembrane domains, there is an extracellular spacer domain and a long, extracellular collagenous domain. The protein may form a trimeric molecule by the association of the collagenous domains of three identical polypeptide chains. Due to its expression in tumor infiltrating macrophages, and its role in inhibiting immune responses within tumor environment, MARCO has been indicated as a potential target for cancer immunotherapy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Macrophage receptor MARCO; Macrophage receptor with collagenous structure; Scavenger receptor class A member 2; scavenger receptor class A, member 2; SR-A6; unnamed protein product
Gene Aliases: MARCO; SCARA2; SR-A6
UniProt ID: (Human) Q9UEW3
Entrez Gene ID: (Human) 8685
immune system process
endocytosis
receptor-mediated endocytosis
phagocytosis, engulfment
cell surface receptor signaling pathway
adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway
apoptotic cell clearance
innate immune response
positive regulation of ERK1 and ERK2 cascade
beta-amyloid clearance
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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