|Tested species reactivity||Human|
|Published species reactivity||Non-human primate, Mouse, Human|
|Host / Isotype||Mouse / IgG2b, kappa|
|Immunogen||Recombinant hMART-1 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100 - 1:200|
|Western Blot (WB)||1:50 - 1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-15237 targets MART-1 in FACS, IHC (P), IP, and WB applications and shows reactivity with Human samples.
The MA5-15237 immunogen is recombinant hMART-1 protein.
MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-Ais a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Primary melanoma of the CNS in children is driven by congenital expression of oncogenic NRAS in melanocytes.
MA5-15237 was used in immunohistochemistry to develop a murine model to study the role of melanocytic expression of oncogenic NRAS in the development of childhood primary melanoma of the CNS
|Pedersen M,Küsters-Vandevelde HV,Viros A,Groenen PJ,Sanchez-Laorden B,Gilhuis JH,van Engen-van Grunsven IA,Renier W,Schieving J,Niculescu-Duvaz I,Springer CJ,Küsters B,Wesseling P,Blokx WA,Marais R||Cancer discovery (3:458)||2013|
Intraglomerular crescentic metastases of malignant melanoma.
MA5-15237 was used in immunohistochemistry to report on a case of intraglomerular crescentic metastases of malignant melanoma
|Ozluk Y,Kilicaslan I,Kazancioglu R,Demirkesen C,Derin D,Mandel NM||Human pathology (42:2025)||2011|
Cellular blue nevus of the uterus: a case report and review of the literature.
MA5-15237 was used in immunohistochemistry to report on a case of cellular blue nevus of the uterus
|Eskue K,Prieto VG,Malpica A||International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists (29:583)||2010|
Regulation of human skin pigmentation in situ by repetitive UV exposure: molecular characterization of responses to UVA and/or UVB.
MA5-15237 was used in immunohistochemistry to study the molecular responses of the skin pigmentation system to repeated exposure to UVA and UVB
|Choi W,Miyamura Y,Wolber R,Smuda C,Reinhold W,Liu H,Kolbe L,Hearing VJ||The Journal of investigative dermatology (130:1685)||2010|
Glomus tumor and glomangioma of the nerve. Report of two cases.
MA5-15237 was used in immunohistochemistry to report on two cases of glomus tumor and glomangioma of the nerve
|Scheithauer BW,Rodriguez FJ,Spinner RJ,Dyck PJ,Salem A,Edelman FL,Amrami KK,Fu YS||Journal of neurosurgery (108:348)||2008|
Dynamic assembly of chromatin complexes during cellular senescence: implications for the growth arrest of human melanocytic nevi.
MA5-15237 was used in immunohistochemistry to examine the role of retinoblastoma pathway in cellular senescence
|Bandyopadhyay D,Curry JL,Lin Q,Richards HW,Chen D,Hornsby PJ,Timchenko NA,Medrano EE||Aging cell (6:577)||2007|
Accumulation of low-avidity anti-melanocortin receptor 1 (anti-MC1R) CD8+ T cells in the lesional skin of a patient with melanoma-related depigmentation.
MA5-15237 was used in immunohistochemistry to investigat specific CD8+ tissue-infiltrating T cells in a patient with melanoma
|Wankowicz-Kalinska A,Mailliard RB,Olson K,Graham F,Edington H,Kirkwood JM,Martinek S,Das PK,Storkus WJ||Melanoma research (16:165)||2006|
McCune-Albright syndrome in a boy may present with a monolateral macroorchidism as an early and isolated clinical manifestation.
MA5-15237 was used in immunohistochemistry to report on a case of McCune-Albright syndrome with monolateral macroorchidism
|Arrigo T,Pirazzoli P,De Sanctis L,Leone O,Wasniewska M,Messina MF,De Luca F||Hormone research (65:114)||2006|
Immunogenicity, including vitiligo, and feasibility of vaccination with autologous GM-CSF-transduced tumor cells in metastatic melanoma patients.
MA5-15237 was used in immunohistochemistry to study the therapeutic potential of autologous tumor cells virally transduced with GM-CSF in metastatic melanoma patients
|Luiten RM,Kueter EW,Mooi W,Gallee MP,Rankin EM,Gerritsen WR,Clift SM,Nooijen WJ,Weder P,van de Kasteele WF,Sein J,van den Berk PC,Nieweg OE,Berns AM,Spits H,de Gast GC||Journal of clinical oncology : official journal of the American Society of Clinical Oncology (23:8978)||2005|
Mechanisms of skin tanning in different racial/ethnic groups in response to ultraviolet radiation.
MA5-15237 was used in immunohistochemistry to study the mechanisms by which UV radiation stimulates skin pigmentation in different ethnic groups
|Tadokoro T,Yamaguchi Y,Batzer J,Coelho SG,Zmudzka BZ,Miller SA,Wolber R,Beer JZ,Hearing VJ||The Journal of investigative dermatology (124:1326)||2005|
On the role of melanoma-specific CD8+ T-cell immunity in disease progression of advanced-stage melanoma patients.
MA5-15237 was used in immunohistochemistry to study the role of CD8+ T-cell immunity in disease progression of advanced-stage melanoma
|van Oijen M,Bins A,Elias S,Sein J,Weder P,de Gast G,Mallo H,Gallee M,Van Tinteren H,Schumacher T,Haanen J||Clinical cancer research : an official journal of the American Association for Cancer Research (10:4754)||2004|
Intraparenchymal nevus cell aggregates in lymph nodes: a possible diagnostic pitfall with malignant melanoma and carcinoma.
MA5-15237 was used in immunohistochemistry to investigate the aggregation of intraparenchymal nevus cells in lymph nodes
|Biddle DA,Evans HL,Kemp BL,El-Naggar AK,Harvell JD,White WL,Iskandar SS,Prieto VG||The American journal of surgical pathology (27:673)||2003|
Peripheral burst of tumor-specific cytotoxic T lymphocytes and infiltration of metastatic lesions by memory CD8+ T cells in melanoma patients receiving interleukin 12.
MA5-15237 was used in immunohistochemistry to investigate the effect of interleukin 12 administration on tumor-specific cytotoxic T lymphocytes and metastatic lesions
|Mortarini R,Borri A,Tragni G,Bersani I,Vegetti C,Bajetta E,Pilotti S,Cerundolo V,Anichini A||Cancer research (60:3559)||2000|
Vaccination with antigen-transfected, NKT cell ligand-loaded, human cells elicits robust in situ immune responses by dendritic cells.
MA5-15237 was used in western blot to develop human artificial adjuvant vector cells for cancer immunotherapy
|Shimizu K,Mizuno T,Shinga J,Asakura M,Kakimi K,Ishii Y,Masuda K,Maeda T,Sugahara H,Sato Y,Matsushita H,Nishida K,Hanada K,Dorrie J,Schaft N,Bickham K,Koike H,Ando T,Nagai R,Fujii S||Cancer research (73:62)||2013|
Glycoprotein nonmetastatic melanoma protein b, a melanocytic cell marker, is a melanosome-specific and proteolytically released protein.
MA5-15237 was used in western blot to study the proteolytic release of glycoprotein nonmetastatic melanoma protein b from melanosomes
|Hoashi T,Sato S,Yamaguchi Y,Passeron T,Tamaki K,Hearing VJ||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (24:1616)||2010|
|Non-human primate||Not Cited||
Dendritic cell vaccine with mRNA targeted to the proteasome by polyubiquitination.
MA5-15237 was used in western blot to study the therapeutic potential of a dendritic cell vaccine using mRNAs encoding tumor-associated antigens targeted to the proteasome
|Hosoi A,Takeda Y,Sakuta K,Ueha S,Kurachi M,Kimura K,Maekawa R,Kakimi K||Biochemical and biophysical research communications (371:242)||2008|
Computational peptide dissection of Melan-a/MART-1 oncoprotein antigenicity.
MA5-15237 was used in western blot to identify the B cell epitope of a monoclonal antibody against the melanoma-associated-antigen
|Tiwari R,Geliebter J,Lucchese A,Mittelman A,Kanduc D||Peptides (25:1865)||2004|
Identification of monoclonal anti-HMW-MAA antibody linear peptide epitope by proteomic database mining.
MA5-15237 was used in western blot to evaluate a stategy to identify the antigenic sequences in MHC molecules
|Mittelman A,Tiwari R,Lucchese G,Willers J,Dummer R,Kanduc D||The Journal of investigative dermatology (123:670)||2004|
Cyclobutane pyrimidine dimer formation and p53 production in human skin after repeated UV irradiation.
MA5-15237 was used in immunocytochemistry to study the potential protective effects of facultative pigmentation following repeated exposure to UV irradiation
|Yamaguchi Y,Coelho SG,Zmudzka BZ,Takahashi K,Beer JZ,Hearing VJ,Miller SA||Experimental dermatology (17:916)||2008|
Antigenic profiling of glioma cells to generate allogeneic vaccines or dendritic cell-based therapeutics.
MA5-15237 was used in flow cytometry to examine the allogenic glioma cells for the generation of therapeutic vaccines or cellular therapy
|Zhang JG,Eguchi J,Kruse CA,Gomez GG,Fakhrai H,Schroter S,Ma W,Hoa N,Minev B,Delgado C,Wepsic HT,Okada H,Jadus MR||Clinical cancer research : an official journal of the American Association for Cancer Research (13:566)||2007|
MART-1 is required for the function of the melanosomal matrix protein PMEL17/GP100 and the maturation of melanosomes.
MA5-15237 was used in immunoprecipitation to study the role of MART-1 on PMEL17/GP100 function and melanosome maturation
|Hoashi T,Watabe H,Muller J,Yamaguchi Y,Vieira WD,Hearing VJ||The Journal of biological chemistry (280:14006)||2005|