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|Tested species reactivity||Tag|
|Published species reactivity||Eubacteria, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant maltose binding protein|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
PA1-989 detects recombinant maltose binding protein.
PA1-989 has been used successfully in Western blotting to detect the presence of maltose binding protein tagged proteins.
The PA1-989 immunogen is recombinant maltose binding protein.
Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the peptide epitope tag. The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the peptide tag. Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered peptide, thus eliminating the need for antibodies to proteins from each newly cloned gene. Commonly used epitope tags include glutathione-S-transferase (GST), c-myc, 6-histidine (6X-His), FLAG, green fluorescent protein (GFP), red fluorescent protein (RFP, DSRed), maltose binding protein (MBP), influenza A virus haemagglutinin (HA), b-galactosidase, and GAL4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The Bacterial Sec Pathway of Protein Export: Screening and Follow-Up.
PA1-989 was used in western blot to screen for compounds that inhibit Sec-dependent secretion in E. coli by assessing intracellular beta-galactosidase activity
|Crowther GJ,Weller SM,Jones JC,Weaver T,Fan E,Van Voorhis WC,Rosen H||Journal of biomolecular screening (20:921)||2015|
Hepatitis C virus RNA replication and virus particle assembly require specific dimerization of the NS4A protein transmembrane domain.
PA1-989 was used in western blot to study the role HCV NS4A transmembrane dimerization in viral RNA replication and particle assembly
|Kohlway A,Pirakitikulr N,Barrera FN,Potapova O,Engelman DM,Pyle AM,Lindenbach BD||Journal of virology (88:628)||2014|