|Tested species reactivity||Tag|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant MBP fusion protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
MA5-14122 targets Maltose Binding Protein in IF and WB applications and shows reactivity with Tag samples.
The MA5-14122 immunogen is purified recombinant MBP fusion protein.
The approach of epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. The insertion of a MBP tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP-tagged product. Cleavage by factor Xa separates MBP from its partner protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
AKAP150 signaling complex promotes suppression of the M-current by muscarinic agonists.
MA5-14122 was used in western blot to study the role of the AKAP150 signaling complex in suppression of the M-current by muscarinic agonists
|Hoshi N,Zhang JS,Omaki M,Takeuchi T,Yokoyama S,Wanaverbecq N,Langeberg LK,Yoneda Y,Scott JD,Brown DA,Higashida H||Nature neuroscience (6:564)||2003|