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|Tested species reactivity||Human, Mouse, Non-human primate, Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant fragment of human MCM2 expressed in E. Coli.|
|Contains||0.03% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1/200 - 1/400|
|Immunohistochemistry (IHC)||1/200 - 1/1000|
|Western Blot (WB)||1/500 - 1/2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-15895 targets MCM2 in FACS, IHC, and WB applications and shows reactivity with Human, mouse, Non-human primate, and Rat samples.
The MA5-15895 immunogen is purified recombinant fragment of human MCM2 expressed in E. Coli. .
MA5-15895 detects MCM2 which has a predicted molecular weight of approximately 125kDa.
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
BM28, CCNL1, CDCL1, D3S3194, MITOTIN, cdc19, DNA replication licensing factor MCM2; cell devision cycle-like 1; cyclin-like 1; DNA replication licensing factor MCM2; MCM2 minichromosome maintenance deficient 2, mitotin; mini chromosome maintenance deficient 2; minichromosome maintenance deficient 2 (mitotin); minichromosome maintenance deficient 2 mitotin; Minichromosome maintenance protein 2 homolog; Nuclear protein BM28
AA959861; AW476101; BM28; CCNL1; cdc19; CDCL1; D3S3194; DFNA70; KIAA0030; MCM2; Mcmd2; MITOTIN; mKIAA0030